LOCUS       V002622                12543 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002622
VERSION     V002622
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12543)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..29
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     110..126
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        230..899
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    911..966
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             968..4318
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83519.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            968..4318
                     /gene="Rep"
                     /label="Rep"
     CDS             4394..5812
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83520.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4394..5812
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5796..6599
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6683..6706
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             6743..6766
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             6800..6817
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             6821..6853
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             6857..6880
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
     protein_bind    6890..7014
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        7039..7069
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             7123..7779
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             8124..8426
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8470..8594)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8839..8883
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      8926..9136
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    9206..9230
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9385..10530)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10832..11623)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11835..12470)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
       61 tgcggacgtt tttaatgtac tggggtggtt ttggtaccgg gccccccctc gaggtcgacg
      121 gtatcgataa gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacacacttg
      181 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
      241 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
      301 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
      361 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      421 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      481 ataacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      541 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      601 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      661 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      721 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      781 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
      841 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg
      901 tatttttaca acaattacca acaacaacaa acaacagaca acattacaat tactatttac
      961 aattacaatg gcatacacac agacagctac cacatcagct ttgctggaca ctgtccgagg
     1021 aaacaactcc ttggtcaatg atctagcaaa gcgtcgtctt tacgacacag cggttgaaga
     1081 gtttaacgct cgtgaccgca ggcccaaggt gaacttttca aaagtaataa gcgaggagca
     1141 gacgcttatt gctacccggg cgtatccaga attccaaatt acattttata acacgcaaaa
     1201 tgccgtgcat tcgcttgcag gtggattgcg atctttagaa ctggaatatc tgatgatgca
     1261 aattccctac ggatcattga cttatgacat aggcgggaat tttgcatcgc atctgttcaa
     1321 gggacgagca tatgtacact gctgcatgcc caacctggac gttcgagaca tcatgcggca
     1381 cgaaggccag aaagacagta ttgaactata cctttctagg ctagagagag gggggaaaac
     1441 agtccccaac ttccaaaagg aagcatttga cagatacgca gaaattcctg aagacgctgt
     1501 ctgtcacaat actttccaga catgcgaaca tcagccgatg cagcaatcag gcagagtgta
     1561 tgccattgcg ctacacagca tatatgacat accagccgat gagttcgggg cggcactctt
     1621 gaggaaaaat gtccatacgt gctatgccgc tttccacttc tccgagaacc tgcttcttga
     1681 agattcatgc gtcaatttgg acgaaatcaa cgcgtgtttt tcgcgcgatg gagacaagtt
     1741 gaccttttct tttgcatcag agagtactct taattactgt catagttatt ctaatattct
     1801 taagtatgtg tgcaaaactt acttcccggc ctctaataga gaggtttaca tgaaggagtt
     1861 tttagtcacc agagttaata cctggttttg taagttttct agaatagata cttttctttt
     1921 gtacaaaggt gtggcccata aaagtgtaga tagtgagcag ttttatactg caatggaaga
     1981 cgcatggcat tacaaaaaga ctcttgcaat gtgcaacagc gagagaatcc tccttgagga
     2041 ttcatcatca gtcaattact ggtttcccaa aatgagggat atggtcatcg taccattatt
     2101 cgacatttct ttggagacta gtaagaggac gcgcaaggaa gtcttagtgt ccaaggattt
     2161 cgtgttcaca gtgcttaacc acattcgaac ataccaggcg aaagctctta catacgcaaa
     2221 tgttttgtcc ttcgtcgaat cgattcgatc gagggtaatc attaacggtg tgacagcgag
     2281 gtccgaatgg gatgtggaca aatctttgtt acaatccttg tccatgacgt tttacctgca
     2341 tactaagctt gccgttctaa aggatgactt actgattagc aagtttagtc tcggttcgaa
     2401 aacggtgtgc cagcatgtgt gggatgagat ttcgctggcg tttgggaacg catttccctc
     2461 cgtgaaagag aggctcttga acaggaaact tatcagagtg gcaggcgacg cattagagat
     2521 cagggtgcct gatctatatg tgaccttcca cgacagatta gtgactgagt acaaggcctc
     2581 tgtggacatg cctgcgcttg acattaggaa gaagatggaa gaaacggaag tgatgtacaa
     2641 tgcactttca gaattatcgg tgttaaggga gtctgacaaa ttcgatgttg atgttttttc
     2701 ccagatgtgc caatctttgg aagttgaccc aatgacggca gcgaaggtta tagtcgcggt
     2761 catgagcaat gagagcggtc tgactctcac atttgaacga cctactgagg cgaatgttgc
     2821 gctagcttta caggatcaag agaaggcttc agaaggtgca ttggtagtta cctcaagaga
     2881 agttgaagaa ccgtccatga agggttcgat ggccagagga gagttacaat tagctggtct
     2941 tgctggagat catccggaat cgtcctattc taagaacgag gagatagagt ctttagagca
     3001 gtttcatatg gcgacggcag attcgttaat tcgtaagcag atgagctcga ttgtgtacac
     3061 gggtccgatt aaagttcagc aaatgaaaaa ctttatcgat agcctggtag catcactatc
     3121 tgctgcggtg tcgaatctcg tcaagatcct caaagataca gctgctattg accttgaaac
     3181 ccgtcaaaag tttggagtct tggatgttgc atctaggaag tggttaatca aaccaacggc
     3241 caagagtcat gcatggggtg ttgttgaaac ccacgcgagg aagtatcatg tggcgctttt
     3301 ggaatatgat gagcagggtg tggtgacatg cgatgattgg agaagagtag ctgttagctc
     3361 tgagtctgtt gtttattccg acatggcgaa actcagaact ctgcgcagac tgcttcgaaa
     3421 cggagaaccg catgtcagta gcgcaaaggt tgttcttgtg gacggagttc cgggctgtgg
     3481 aaaaaccaaa gaaattcttt ccagggttaa ttttgatgaa gatctaattt tagtacctgg
     3541 gaagcaagcc gcggaaatga tcagaagacg tgcgaattcc tcagggatta ttgtggccac
     3601 gaaggacaac gttaaaaccg ttgattcttt catgatgaat tttgggaaaa gcacacgctg
     3661 tcagttcaag aggttattca ttgatgaagg gttgatgttg catactggtt gtgttaattt
     3721 tcttgtggcg atgtcattgt gcgaaattgc atatgtttac ggagacacac agcagattcc
     3781 atacatcaat agagtttcag gattcccgta ccccgcccat tttgccaaat tggaagttga
     3841 cgaggtggag acacgcagaa ctactctccg ttgtccagcc gatgtcacac attatctgaa
     3901 caggagatat gagggctttg tcatgagcac ttcttcggtt aaaaagtctg tttcgcagga
     3961 gatggtcggc ggagccgccg tgatcaatcc gatctcaaaa cccttgcatg gcaagatcct
     4021 gacttttacc caatcggata aagaagctct gctttcaaga gggtattcag atgttcacac
     4081 tgtgcatgaa gtgcaaggcg agacatactc tgatgtttca ctagttaggt taacccctac
     4141 accggtctcc atcattgcag gagacagccc acatgttttg gtcgcattgt caaggcacac
     4201 ctgttcgctc aagtactaca ctgttgttat ggatccttta gttagtatca ttagagatct
     4261 agagaaactt agctcgtact tgttagatat gtataaggtc gatgcaggaa cacaatagca
     4321 attacagatt gactcggtgt tcaaaggttc caatcttttt gttgcagcgc caaagactgg
     4381 tgatatttct gatatgcagt tttactatga taagtgtctc ccaggcaaca gcaccatgat
     4441 gaataatttt gatgctgtta ccatgaggtt gactgacatt tcattgaatg tcaaagattg
     4501 catattggat atgtctaagt ctgttgctgc gcctaaggat caaatcaaac cactaatacc
     4561 tatggtacga acggcggcag aaatgccacg ccagactgga ctattggaaa atttagtggc
     4621 gatgattaaa agaaacttta acgcacccga gttgtctggc atcattgata ttgaaaatac
     4681 tgcatctttg gttgtagata agttttttga tagttatttg cttaaagaaa aaagaaaacc
     4741 aaataaaaat gtttctttgt tcagtagaga gtctctcaat agatggttag aaaagcagga
     4801 acaggtaaca ataggccagc tcgcagattt tgattttgtg gatttgccag cagttgatca
     4861 gtacagacac atgattaaag cacaacccaa acaaaagttg gacacttcaa tccaaacgga
     4921 gtacccggct ttgcagacga ttgtgtacca tcaaaaaaga tcaatgcaat atcgcccgtt
     4981 gttagtgagc ttaccaggca attactggac agtgttgatt cgagcagatt ttgttttcac
     5041 aagaaagaca ccagcgcaga ttgaggattt cttcggagat ctcgacagtc atgtgccgat
     5101 ggatgtcttg gagctggata tatcaaaata cgacaaatct cagaatgaat tccactgtgc
     5161 agtagaatac gagatctggc gaagattggg tttcgaagac ttcttgggag aagtttggaa
     5221 acaagggcat agaaagacca ccctcaagga ttataccgca ggtataaaaa cttgcatctg
     5281 gtatcaaaga aagagcgggg acgtcacgac gttcattgga aacactgtga tcattgctgc
     5341 atgtttggcc tcgatgcttc cgatggagaa aataatcaaa ggagcctttt gcggtgacga
     5401 tagtctgctg tactttccaa agggttgtga gtttccggat gtgcaacact ccgcgaatct
     5461 tatgtggaat tttgaagcaa aactgtttaa aaaacagtat ggatactttt gcggaagata
     5521 tgtaatacat cacgacagag gatgcattgt gtattacgat cccctaaagt tgatctcgaa
     5581 acttggtgct aaacacatca aggattggga acacttggag gagttcagaa ggtctctttg
     5641 tgatgttgct gtttcgttga acaattgtgc gtattacaca cagttggacg acgctgtatg
     5701 ggaggttcat aagaccgccc ctccaggttc gtttgtttat aaaagtctgg tgaagtattt
     5761 gtctgataaa gttcttttta gaagtttgtt tatagatggc tctagttgtt aaaggaaaag
     5821 tgaatatcaa tgagtttatc gacctgacaa aaatggagaa gatcttaccg tcgatgttta
     5881 cccctgtaaa gagtgttatg tgttccaaag ttgataaaat aatggttcat gagaatgagt
     5941 cattgtcagg ggtgaacctt cttaaaggag ttaagcttat tgatagtgga tacgtctgtt
     6001 tagccggttt ggtcgtcacg ggcgagtgga acttgcctga caattgcaga ggaggtgtga
     6061 gcgtgtgtct ggtggacaaa aggatggaaa gagccgacga ggccattctc ggatcttact
     6121 acacagcagc tgcaaagaaa agatttcagt tcaaggtcgt tcccaattat gctataacca
     6181 cccaggacgc gatgaaaaac gtctggcaag ttttagttaa tattagaaat gtgaagatgt
     6241 cagcgggttt ctgtccgctt tctctggagt ttgtgtcggt gtgtattgtt tatagaaata
     6301 atataaaatt aggtttgaga gagaagatta caaacgtgag agacggaggg cccatggaac
     6361 ttacagaaga agtcgttgat gagttcatgg aagatgtccc tatgtcgatc aggcttgcaa
     6421 agtttcgatc tcgaaccgga aaaaagagtg atgtccgcaa agggaaaaat agtagtagtg
     6481 atcggtcagt gccgaacaag aactatagaa atgttaagga ttttggggga atgagtttta
     6541 aaaagaataa tttaatcgat gatgattcgg aggctactgt cgccgaatcg gattcgtttt
     6601 aaatagatct tacagtatca ctactccatc tcagttcgtg ttcttgtcat taattaacat
     6661 ggctggaagt actggagctt cttggtctca tcctcaattt gaaaagggag gaggttctgg
     6721 aggtggatct ggtggaggat catggtctca tccacagttc gaaaagggag ctggatctag
     6781 gcctattatg cggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
     6841 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatcatcaaa caagtttgta
     6901 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     6961 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     7021 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     7081 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     7141 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     7201 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     7261 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     7321 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     7381 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     7441 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     7501 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     7561 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     7621 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     7681 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     7741 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta
     7801 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     7861 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     7921 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     7981 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     8041 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     8101 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     8161 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     8221 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     8281 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     8341 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     8401 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     8461 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     8521 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     8581 ttgtacaaag tggttcgatc ctagggcggc cgcggtcctg caacttgagg tagtcaagat
     8641 gcataataaa taacggattg tgtccgtaat cacacgtggt gcgtacgata acgcatagtg
     8701 tttttccctc cacttaaatc gaagggttgt gtcttggatc gcgcgggtca aatgtatatg
     8761 gttcatatac atccgcaggc acgtaataaa gcgaggggtt cgaatccccc cgttaccccc
     8821 ggtaggggcc caggtacccg gatgtgtttt ccgggctgat gagtccgtga ggacgaaacc
     8881 tggctgcagg catgcaagct tggcgtaatc atggtcatag cctagagtcc gcaaaaatca
     8941 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     9001 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     9061 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     9121 aaccaaaatc cagtgacctg cagcccggcc gggggatcca ctagcagatt gtcgtttccc
     9181 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     9241 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     9301 catttgtatg tgcatgccaa ccacaggaga tctcagtaaa gcgctggctg aacccccagc
     9361 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
     9421 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
     9481 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
     9541 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
     9601 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
     9661 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
     9721 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
     9781 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
     9841 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
     9901 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
     9961 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
    10021 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
    10081 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
    10141 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
    10201 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
    10261 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
    10321 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
    10381 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
    10441 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
    10501 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
    10561 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
    10621 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
    10681 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
    10741 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
    10801 gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt
    10861 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    10921 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    10981 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    11041 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    11101 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    11161 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    11221 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    11281 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    11341 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    11401 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    11461 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    11521 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    11581 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    11641 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    11701 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    11761 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat
    11821 gctgccaact cgagagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
    11881 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
    11941 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
    12001 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
    12061 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
    12121 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
    12181 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
    12241 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
    12301 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
    12361 gcggccggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    12421 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc tagtggactg
    12481 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    12541 tgg
//
