LOCUS       40924_43513        6718 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector for constitutive low-level expression of 
            intracellular red firefly luciferase under control of the HSV TK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..777
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             790..2433
                     /codon_start=1
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
                     /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
                     NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
                     APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
                     QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
                     HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
                     YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
                     MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
                     VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
     polyA_signal    2462..2573
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2696..3025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3073..3120
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3139..3735
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3868..4001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4045..4902)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5166..5754
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6024..6040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6496..6512
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    6627..6718
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtctcg aggaattcca agcttaatga gtcttcggac ctcgcggggg ccgcttaagc
       61 ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga
      121 ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt
      181 aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc
      241 cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc
      301 ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt
      361 atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg
      421 cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac
      481 ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga
      541 gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
      601 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
      661 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
      721 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaagga
      781 tccgccacca tggaaaatat ggaaaacgac gagaacatcg tggtgggccc caagcccttc
      841 taccccatcg aggaaggcag cgccggcacc cagctgcgga agtacatgga aagatacgcc
      901 aagctgggcg ccattgcctt caccaacgcc gtgaccggcg tggactacag ctacgccgag
      961 tacctggaaa agagctgctg cctgggcaag gctctgcaga actacggcct ggtggtggac
     1021 ggccggatcg ccctgtgcag cgagaactgc gaggaattct tcatccccgt gatcgccggc
     1081 ctgttcatcg gcgtgggcgt ggctcccacc aacgagatct acaccctgcg ggagctggtg
     1141 cacagcctgg gcatcagcaa gcccaccatc gtgttcagca gcaagaaggg cctggacaaa
     1201 gtcatcaccg tgcagaaaac cgtgaccacc atcaagacca tcgtgatcct ggacagcaag
     1261 gtggactacc ggggctacca gtgcctggac accttcatca agcggaacac cccccctggc
     1321 ttccaggcca gcagcttcaa gaccgtggag gtggaccgga aagaacaggt ggccctgatc
     1381 atgaacagca gcggcagcac cggcctgccc aagggcgtgc agctgaccca cgagaacacc
     1441 gtgacccggt tcagccacgc cagggacccc atctacggca accaggtgtc ccccggcacc
     1501 gccgtgctga ccgtggtgcc cttccaccac ggcttcggca tgttcaccac cctgggctac
     1561 ctgatctgcg gcttccgggt ggtgatgctg accaagttcg acgaggaaac cttcctgaaa
     1621 accctgcagg actacaagtg cacctacgtg attctggtgc ccaccctgtt cgccatcctg
     1681 aacaagagcg agctgctgaa caagtacgac ctgagcaacc tggtggagat cgccagcggc
     1741 ggagcccccc tgagcaaaga agtgggagag gccgtcgcca ggcggttcaa tctgcccggc
     1801 gtgcggcagg gctacggcct gaccgagaca accagcgcca tcatcatcac ccccgagggc
     1861 gacgacaagc ctggagccag cggcaaggtg gtgcccctgt tcaaggccaa agtgatcgac
     1921 ctggacacca agaagagcct gggccccaac agacggggcg aagtgtgcgt gaagggcccc
     1981 atgctgatga agggctacgt gaacaacccc gaggccacca aagagctgat cgacgaagag
     2041 ggctggctgc acaccggcga catcggctac tacgacgaag agaagcactt cttcatcgtg
     2101 gaccggctga agagcctgat caagtacaag ggctatcagg tgccccctgc cgagctggaa
     2161 agcgtcctgc tgcagcaccc cagcatcttc gacgccggcg tggccggggt gccagatcct
     2221 gtggccggcg agctgcctgg cgccgtggtg gtgctggaat ccggcaagaa catgaccgag
     2281 aaagaagtga tggactacgt cgccagccag gtgtccaacg ccaagcggct gagaggcggc
     2341 gtgagattcg tggacgaagt gccaaagggc ctgaccggca agatcgacgg cagggccatc
     2401 cgggagatcc tgaagaaacc cgtggccaag atgtgatgag cggccgcaaa atcagcctcg
     2461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
     2581 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2641 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     2701 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2761 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2821 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2881 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2941 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     3001 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     3061 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     3121 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3181 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3241 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     3421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     3481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     3541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3601 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
     3661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3721 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     3781 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     3841 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     3901 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3961 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     4021 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
     4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4141 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
     4201 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4321 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
     4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tttttcaata
     4921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4981 gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc
     5041 gagcccattt atacctgaat atggctcata acaccccttg ctcatgacca aaatccctta
     5101 acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5281 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttagcccac
     5341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag
     5941 agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg
     6001 tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg
     6061 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg
     6121 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc
     6181 actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt
     6241 aaataagata cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta
     6301 tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat
     6361 ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg tcaacgataa
     6421 tacaaaatat aatacaaact ataagatgtt atcagtattt attatcattt agaataaatt
     6481 ttgtgtcgcc cttaattgtg agcggataac aattacgagc ttcatgcaca gtggcgttga
     6541 cattgattat tgactagcat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6601 attaccgcca tgcattagtt attaataaca tacgctctcc atcaaaacaa aacgaaacaa
     6661 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//
