LOCUS       40924_43503        5066 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(29..163)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1450..1498)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1543..2577)
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRGDRE
                     MGEAN"
     promoter        complement(2612..2757)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(3214..3802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3976..4833)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4834..4938)
                     /label=AmpR promoter
ORIGIN
        1 ctggcgtaat agcgaagagg cccgcaccga tccagacatg ataagataca ttgatgagtt
       61 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      121 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      181 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      241 ctacaaatgt ggtatggctg attatgatcc ccaggaagct cctctgtgtc ctcataaacc
      301 ctaacctcct ctacttgaga ggacattcca atcataggct gcccatccac cctctgtgtc
      361 ctcctgttaa ttaggtcact taacaaaaag gaaattgggt aggggttttt cacagaccgc
      421 tttctaaggg taattttaaa atatctggga agtcccttcc actgctgtgt tccagaagtg
      481 ttggtaaaca gcccacaaat gtcaacagca gaaacataca agctgtcagc tttgcacaag
      541 ggcccaacac cctgctcatc aagaagcact gtggttgctg tgttagtaat gtgcaaaaca
      601 ggaggcacat tttccccacc tgtgtaggtt ccaaaatatc tagtgttttc atttttactt
      661 ggatcaggaa cccagcactc cactggataa gcattatcct tatccaaaac agccttgtgg
      721 tcagtgttca tctgctgact gtcaactgta gcattttttg gggttacagt ttgagcagga
      781 tatttggtcc tgtagtttgc taacacaccc tgcagctcca aaggttcccc accaacagca
      841 aaaaaatgaa aatttgaccc ttgaatgggt tttccagcac cattttcatg agttttttgt
      901 gtccctgaat gcaagtttaa catagcagtt accccaataa cctcagtttt aacagtaaca
      961 gcttcccaca tcaaaatatt tccacaggtt aagtcctcat ttaaattagg caaaggaatt
     1021 cctcgacctg cagcccaagc ttggcactgg cgccagaaat ccgcgcggtg gtttttgggg
     1081 gtcgggggtg tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc
     1141 catgcccagg ccatccaaaa accatgggtc tgtctgctca gtccagtcgt ggacctgacc
     1201 ccacgcaacg cccaaaagaa taacccccac gaaccataaa ccattcccca tgggggaccc
     1261 cgtccctaac ccacggggcc cgtggctatg gcgggcttgc cgccccgacg ttggctgcga
     1321 gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa acgcgggcgt
     1381 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
     1441 cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgtc
     1501 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct cccccatctc
     1561 ccgatcccca cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1621 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1681 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1741 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1801 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1861 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1921 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1981 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     2041 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     2101 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     2161 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2221 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2281 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2341 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2401 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2461 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2521 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
     2581 aagctgatct tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg gtcgctcggt
     2641 gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc gccgccccga
     2701 ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat
     2761 agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg
     2821 ggccacgggg atgaagcagc ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     2881 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     2941 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcggggcc gacgcgctgg
     3001 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     3061 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     3121 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagcgcc agcaaaaggc
     3181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4261 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     4321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4561 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     4621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4981 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     5041 attctcatgt ttgacagctt atcatc
//
