LOCUS       40924_43488        5627 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector for constitutive low-level expression of secreted 
            Gaussia luciferase under control of the HSV TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..777
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             791..1345
                     /codon_start=1
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     polyA_signal    1371..1482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1605..1934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1982..2029
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2048..2644
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2777..2910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2954..3811)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4075..4663
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4933..4949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5405..5421
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5536..5627
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtctcg aggaattcca agcttaatga gtcttcggac ctcgcggggg ccgcttaagc
       61 ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga
      121 ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt
      181 aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc
      241 cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc
      301 ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt
      361 atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg
      421 cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac
      481 ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga
      541 gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
      601 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
      661 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
      721 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaagga
      781 tccagccacc atgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc
      841 caagcccacc gagaacaacg aagacttcaa catcgtggcc gtggccagca acttcgcgac
      901 cacggatctc gatgctgacc gcgggaagtt gcccggcaag aagctgccgc tggaggtgct
      961 caaagagatg gaagccaatg cccggaaagc tggctgcacc aggggctgtc tgatctgcct
     1021 gtcccacatc aagtgcacgc ccaagatgaa gaagttcatc ccaggacgct gccacaccta
     1081 cgaaggcgac aaagagtccg cacagggcgg cataggcgag gcgatcgtcg acattcctga
     1141 gattcctggg ttcaaggact tggagcccat ggagcagttc atcgcacagg tcgatctgtg
     1201 tgtggactgc acaactggct gcctcaaagg gcttgccaac gtgcagtgtt ctgacctgct
     1261 caagaagtgg ctgccgcaac gctgtgcgac ctttgccagc aagatccagg gccaggtgga
     1321 caagatcaag ggggccggtg gtgactaagc ggccgcaaaa tcagcctcga ctgtgccttc
     1381 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1441 cactcccact gtcctttcct aataaaatga ggaaattgca tcacaacact caaccctatc
     1501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1561 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     1621 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1681 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1741 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1801 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1861 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1921 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     1981 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2041 ctaaaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     2101 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     2161 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     2221 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     2281 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2341 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2401 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2461 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2521 cttcctggag acatccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2581 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     2641 tgcctgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     2701 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     2761 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2881 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     2941 atcatggtca ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3061 catctggccc cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat
     3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3241 gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3781 taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca
     3841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3901 aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta
     3961 tacctgaata tggctcataa caccccttgc tcatgaccaa aatcccttaa cgtgagttac
     4021 gcgcgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttagcccacc acttcaagaa
     4261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4741 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4801 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaggcgaga gtagggaact
     4861 gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac
     4921 tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat
     4981 aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg
     5041 ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga
     5101 tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac
     5161 gttgcttttt cgattgatga acacctataa ttaaactatt catctattat ttatgatttt
     5221 ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa
     5281 taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata
     5341 atacaaacta taagatgtta tcagtattta ttatcattta gaataaattt tgtgtcgccc
     5401 ttaattgtga gcggataaca attacgagct tcatgcacag tggcgttgac attgattatt
     5461 gactagcatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     5521 gcattagtta ttaataacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     5581 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
