LOCUS       40924_43483        5626 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector for constitutive low-level expression of secreted 
            Gaussia-Dura luciferase under control of the HSV TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..777
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             790..1344
                     /codon_start=1
                     /label=Gaussia-Dura Luc
                     /note="enhanced variant of secreted Gaussia luciferase"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     polyA_signal    1370..1481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1604..1933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1981..2028
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2047..2643
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2776..2909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2953..3810)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4074..4662
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4932..4948
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5404..5420
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5535..5626
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtctcg aggaattcca agcttaatga gtcttcggac ctcgcggggg ccgcttaagc
       61 ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga
      121 ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt
      181 aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc
      241 cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc
      301 ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt
      361 atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg
      421 cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac
      481 ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga
      541 gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
      601 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
      661 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
      721 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaagga
      781 tccgccacca tgggagtcaa agttctgttt gccctgatct gcatcgctgt ggccgaggcc
      841 aagcccaccg agaacaacga agacttcaac atcgtggccg tggccagcaa cttcgcgacc
      901 acggatctcg atgctgaccg cgggaagttg cccggcaaga agctgccgct ggaggtgctc
      961 aaagagttgg aagccaatgc ccggaaagct ggctgcacca ggggctgtct gatctgcctg
     1021 tcccacatca agtgcacgcc caagatgaag aagttcatcc caggacgctg ccacacctac
     1081 gaaggcgaca aagagtccgc acagggcggc ataggcgagg cgatcgtcga cattcctgag
     1141 attcctgggt tcaaggactt ggagcccttg gagcagttca tcgcacaggt cgatctgtgt
     1201 gtggactgca caactggctg cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc
     1261 aagaagtggc tgccgcaacg ctgtgcgacc tttgccagca agatccaggg ccaggtggac
     1321 aagatcaagg gggccggtgg tgactaagcg gccgcaaaat cagcctcgac tgtgccttct
     1381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1441 actcccactg tcctttccta ataaaatgag gaaattgcat cacaacactc aaccctatct
     1501 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1561 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     1621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1681 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1741 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     1801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     1861 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     1921 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     1981 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     2041 taaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2101 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2161 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2221 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2281 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2341 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2401 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2461 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2521 ttcctggaga catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2581 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2641 gcctgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     2701 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     2761 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2821 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2881 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     2941 tcatggtcat taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3061 atctggcccc agcgctgcga tgataccgcg agaaccacgc tcaccggctc cggatttatc
     3121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3241 tttgcgcaac gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3781 aagggcgaca cggaaatgtt gaatactcat attcttcctt tttcaatatt attgaagcat
     3841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3901 aataggggtc agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat
     3961 acctgaatat ggctcataac accccttgct catgaccaaa atcccttaac gtgagttacg
     4021 cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4201 gcgcagatac caaatactgt tcttctagtg tagccgtagt tagcccacca cttcaagaac
     4261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4441 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4741 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4801 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaggcgagag tagggaactg
     4861 ccaggcatca aactaagcag aaggcccctg acggatggcc tttttgcgtt tctacaaact
     4921 ctttctgtgt tgtaaaacga cggccagtct taagctcggg ccccctgggc ggttctgata
     4981 acgagtaatc gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg
     5041 gggagtttag ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat
     5101 atatgagaat tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg
     5161 ttgctttttc gattgatgaa cacctataat taaactattc atctattatt tatgattttt
     5221 tgtatataca atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat
     5281 aaagggaaaa tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa
     5341 tacaaactat aagatgttat cagtatttat tatcatttag aataaatttt gtgtcgccct
     5401 taattgtgag cggataacaa ttacgagctt catgcacagt ggcgttgaca ttgattattg
     5461 actagcatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     5521 cattagttat taataacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     5581 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
