LOCUS       pTK-GLuc.        5897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector with a constitutive HSV thymidine kinase promoter 
            for expressing secreted Gaussia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pTK-GLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..770
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             802..1356
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     misc_feature    1360..1391
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(1407..1528)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1763..2191
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2205..2535
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2602..3393
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3570..3703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3740..3756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3764..3780)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3788..3818)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3833..3854)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4142..4730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4904..5761)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5762..5866)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctaaa tgagtcttcg gacctcgcgg gggccgctta agcggtggtt
       61 agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac actctcattc ggaggcggct
      121 cggggtttgg tcttggtggc cacgggcacg cagaagagcg ccgcgatcct cttaagcacc
      181 cccccgccct ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg ggccgctgac
      241 tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga cccccgggcg
      301 gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct ccatggggac cgtatacgtg
      361 gacaggctct ggagcatccg cacgactgcg gtgatattac cggagacctt ctgcgggacg
      421 agccgggtca cgcggctgac gcggagcgtc cgttgggcga caaacaccag gacggggcac
      481 aggtacacta tcttgtcacc cggaggcgcg agggactgca ggagcttcag ggagtggcgc
      541 agctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
      601 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
      661 aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag
      721 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa aagcttggta
      781 ccgagctcgg atccagccac catgggagtc aaagttctgt ttgccctgat ctgcatcgct
      841 gtggccgagg ccaagcccac cgagaacaac gaagacttca acatcgtggc cgtggccagc
      901 aacttcgcga ccacggatct cgatgctgac cgcgggaagt tgcccggcaa gaagctgccg
      961 ctggaggtgc tcaaagagat ggaagccaat gcccggaaag ctggctgcac caggggctgt
     1021 ctgatctgcc tgtcccacat caagtgcacg cccaagatga agaagttcat cccaggacgc
     1081 tgccacacct acgaaggcga caaagagtcc gcacagggcg gcataggcga ggcgatcgtc
     1141 gacattcctg agattcctgg gttcaaggac ttggagccca tggagcagtt catcgcacag
     1201 gtcgatctgt gtgtggactg cacaactggc tgcctcaaag ggcttgccaa cgtgcagtgt
     1261 tctgacctgc tcaagaagtg gctgccgcaa cgctgtgcga cctttgccag caagatccag
     1321 ggccaggtgg acaagatcaa gggggccggt ggtgactaag cggccgcacc ggtctcgagc
     1381 atgcatctag attcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1441 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1501 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1561 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     1621 aatcgataaa ctagaaataa ttcttactgt catgccaagt aagatgcttt tctgtgctgc
     1681 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     1741 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     1921 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2101 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     2161 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     2221 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     2281 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2461 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2521 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     2581 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     2641 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2701 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2761 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     2821 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2881 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2941 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3001 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3061 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3121 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3181 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3241 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3301 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3361 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     3421 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     3481 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     3541 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     3601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     3721 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3781 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3841 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4321 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4561 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5881 aagtgccacc tgacgtc
//
