LOCUS       pTK-CLuc.        7000 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector with a constitutive HSV thymidine kinase promoter 
            for expressing secreted Cypridina luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pTK-CLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..770
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             801..2459
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     misc_feature    2463..2494
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(2510..2631)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2866..3294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3308..3638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3705..4496
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4673..4806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4843..4859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4867..4883)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4891..4921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4936..4957)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5245..5833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6007..6864)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6865..6969)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctaaa tgagtcttcg gacctcgcgg gggccgctta agcggtggtt
       61 agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac actctcattc ggaggcggct
      121 cggggtttgg tcttggtggc cacgggcacg cagaagagcg ccgcgatcct cttaagcacc
      181 cccccgccct ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg ggccgctgac
      241 tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga cccccgggcg
      301 gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct ccatggggac cgtatacgtg
      361 gacaggctct ggagcatccg cacgactgcg gtgatattac cggagacctt ctgcgggacg
      421 agccgggtca cgcggctgac gcggagcgtc cgttgggcga caaacaccag gacggggcac
      481 aggtacacta tcttgtcacc cggaggcgcg agggactgca ggagcttcag ggagtggcgc
      541 agctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
      601 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
      661 aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag
      721 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa aagcttggta
      781 ccgagctcgg atccgccacc atgaagacct taattcttgc cgttgcatta gtctactgcg
      841 ccactgttca ttgccaggac tgtccttacg aacctgatcc accaaacaca gttccaactt
      901 cctgtgaagc taaagaagga gaatgtattg atagcagctg tggcacctgc acgagagaca
      961 tactatcaga tggactgtgt gaaaataaac caggaaaaac atgttgccga atgtgtcagt
     1021 atgtaattga atgcagagta gaggccgcag gatggtttag aacattctat ggaaagagat
     1081 tccagttcca ggaacctggt acatacgtgt tgggtcaagg aaccaagggc ggcgactgga
     1141 aggtgtccat caccctggag aacctggatg gaaccaaggg ggctgtgctg accaagacaa
     1201 gactggaagt ggctggagac atcattgaca tcgctcaagc tactgagaat cccatcactg
     1261 taaacggtgg agctgaccct atcatcgcca acccgtacac catcggcgag gtcaccatcg
     1321 ctgttgttga gatgccaggc ttcaacatca ccgtcattga gttcttcaaa ctgatcgtga
     1381 tcgacatcct cggaggaaga tctgtaagaa tcgccccaga cacagcaaac aaaggaatga
     1441 tctctggcct ctgtggagat cttaaaatga tggaagatac agacttcact tcagatccag
     1501 aacaactcgc tattcagcct aagatcaacc aggagtttga cggttgtcca ctctatggaa
     1561 atcctgatga cgttgcatac tgcaaaggtc ttctggagcc gtacaaggac agctgccgca
     1621 accccatcaa cttctactac tacaccatct cctgcgcctt cgcccgctgt atgggtggag
     1681 acgagcgagc ctcacacgtg ctgcttgact acagggagac gtgcgctgct cccgaaacta
     1741 gaggaacctg cgttttgtct ggacatactt tctacgatac atttgacaaa gcaagatacc
     1801 aattccaggg tccctgcaag gagattctta tggccgccga ctgtttctgg aacacttggg
     1861 atgtgaaggt ttcacacagg aatgttgact cttacactga agtagagaaa gtacgaatca
     1921 ggaaacaatc gactgtagta gaactcattg ttgatggaaa acagattctg gttggaggag
     1981 aagccgtgtc cgtcccgtac agctctcaga acacttccat ctactggcaa gatggtgaca
     2041 tactgactac agccatccta cctgaagctc tggtggtcaa gttcaacttc aagcaactgc
     2101 tcgtcgtaca tattagagat ccattcgatg gtaagacttg cggtatttgc ggtaactaca
     2161 accaggattt cagtgatgat tcttttgatg ctgaaggagc ctgtgatctg acccccaacc
     2221 caccgggatg caccgaagaa cagaaacctg aagctgaacg actctgcaat agtctcttcg
     2281 ccggtcaaag tgatcttgat cagaaatgta acgtgtgcca caagcctgac cgtgtcgaac
     2341 gatgcatgta cgagtattgc ctgaggggac aacagggttt ctgtgaccac gcatgggagt
     2401 tcaagaaaga atgctacata aagcatggag acaccctaga agtaccagat gaatgcaaat
     2461 aggcggccgc accggtctcg agcatgcatc tagattcgag cagacatgat aagatacatt
     2521 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2581 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2641 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2701 taaaacctct acaaatgtgg taaaatcgat aaactagaaa taattcttac tgtcatgcca
     2761 agtaagatgc ttttctgtgc tgcaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2821 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     2881 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2941 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3001 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
     3061 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3121 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3181 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
     3241 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3301 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     3361 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3421 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3481 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3541 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     3601 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     3661 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     3721 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3781 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3841 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     3901 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3961 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4021 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4081 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4141 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4201 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     4261 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4321 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4381 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4441 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4501 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4561 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4621 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4801 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4861 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4921 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4981 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5041 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5221 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     5461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5641 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5761 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5821 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5881 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5941 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6001 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6061 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6121 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6181 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6241 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6301 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6361 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6421 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6481 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6541 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6601 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6661 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6721 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6781 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6841 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6901 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6961 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
