LOCUS       40924_43468        5667 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTJ1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Damron FH, McKenney ES, Schweizer HP, Goldberg JB.
  TITLE     Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy 
            PBAD-Controlled Gene Expression in Gram-Negative Bacteria
  JOURNAL   Appl. Environ. Microbiol. 79 (2), 718-721 (2013)
  PUBMED    23124231
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Damron FH, McKenney ES, Schweizer H, Goldberg JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2012) Microbiology, Immunology, and Cancer 
            Biology, University of Virginia, PO Box 800734, Charlottesville, VA 
            22908, USA
REFERENCE   3  (bases 1 to 5667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "2"; pages:
            "718-721"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2012) Microbiology, Immunology, and Cancer Biology, 
            University of Virginia, PO Box 800734, Charlottesville, VA 22908, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..885)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        912..1196
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             1223..1245
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1257..1259
                     /label=start codon
                     /note="start codon"
     misc_feature    1271..1327
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     mobile_element  complement(1368..1533)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(1692..1800)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2032..2620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2794..3651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3652..3756)
                     /label=AmpR promoter
     mobile_element  4234..4431
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
     primer_bind     4436..4452
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4480..4574
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4677..4763
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    4814..4861
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4926..5159)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(5484..5512)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   5564..5611
                     /rpt_family="FRT"
     protein_bind    5564..5611
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cccaaattat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
       61 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc
      121 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
      181 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
      241 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
      301 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
      361 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
      421 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
      481 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
      541 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
      601 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
      661 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
      721 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
      781 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
      841 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
      901 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      961 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     1021 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     1081 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     1141 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     1201 gtttttttgg gctagaaata attttgttta actttaagaa ggagatatac atacccatgg
     1261 gatctgataa gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
     1321 caagcttgcg gccgcgggcc cggtacctcg cgaaggcctt gcaggccaac cagataagtg
     1381 aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac
     1441 ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc
     1501 cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt
     1561 tgggcgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1621 tcttccgctt cctcgctcac tgacccgctg cgctcggtcg ttcggctgcg gcgagcggta
     1681 tcagagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     1741 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     1801 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     1861 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     1921 tatgcagcgg aaagtatacc ttaaggaatc ccctgataac gcaggaaaga acatgtgagc
     1981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctcg agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3781 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3841 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     3901 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     3961 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4021 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4081 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4141 ctcttcgcta ttacgccagc tagaggacca gccgcgtaac ctggcaaaat cggttacggt
     4201 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     4261 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     4321 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     4381 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gggttcgagg
     4441 tcgacggtat cgataagcta gcttaattag ctgagcttgg actcctgttg atagatccag
     4501 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     4561 ttattggtga gaatccaagc tagactgcga tgagtggcag ggcggggcgt aattttttta
     4621 aggcagttat tggtgccctt aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa
     4681 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     4741 acgctctcct gagtaggaca aatccgccgc taggagcttg cggcccggac gatgagctcg
     4801 aattggggat cttgaagtac ctattccgaa gttcctattc tctagaaagt ataggaactt
     4861 cagagcgctt ttgaagctga tgtgcttaaa aacttactca atggaataat tctagataat
     4921 tcttaggcca cacgttcaag tgcagccaca ggataaattt gcactgagcc tgggtgggat
     4981 tcggactcga ccgcatagcc ttcaggagtg agttttgtgc aataccaacc gacgacttga
     5041 ccctgccaag cggcaccaga tttcttgcgt acgcgatccc ctaagccaaa ggtggcactc
     5101 aggggaagcg caaactgccc tgcaacggga gcgttggctt catcgctact ttgacccatg
     5161 tcgaatcctt cttgtgaatc tattatggcg acaagcaaat cgagctctga ctgcctaccc
     5221 cacaacaact atcagaaagc accagcacaa cggctgccta actttgtttt agggcgactg
     5281 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     5341 gcttgctgct tggatgcccg aggctagact gtacaaaaaa acagtcataa caagccatga
     5401 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     5461 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     5521 gtgaattatc cattgctgtt gacaaaggga atcaggggat cttgaagttc ctattccgaa
     5581 gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaagctaa ttcgagctcg
     5641 atcatgcatg agctcactag tggcgcg
//
