LOCUS       V002627                 4026 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002627
VERSION     V002627
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Finke S, Conzelmann KK.
  TITLE     Virus promoters determine interference by defective RNAs: selective
            amplification of mini-RNA vectors and rescue from cDNA by a 3'
            copy-back ambisense rabies virus
  JOURNAL   J. Virol. 73 (5), 3818-3825 (1999)
   PUBMED   10196276
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4026)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 4026)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "1999"; volume: "73"; issue: "5"; pages: "3818-3825"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..164
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        171..189
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    240..701
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             706..1596
                     /gene="P"
                     /label="Phosphoprotein"
                     /note="Phosphoprotein from Rabies virus (strain SAD B19).
                     Accession#: P16286"
     terminator      1712..1759
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(1782..1798)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(1835..1853)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1874..1890)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1898..1914)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1922..1952)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1967..1988)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2276..2864)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3038..3895)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3896..4000)
                     /label="AmpR promoter"
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      181 cactataggg cgaattaatt ccggttattt tccaccatat tgccgtcttt tggcaatgtg
      241 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      301 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      361 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      421 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      481 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      541 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      601 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
      661 accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatgag caagatcttt
      721 gtcaatccta gtgctattag agccggtctg gccgatcttg agatggctga agaaactgtt
      781 gatctgatca atagaaatat cgaagacaat caggctcatc tccaagggga acccatagag
      841 gtggacaatc tccctgagga tatggggcga cttcacctgg atgatggaaa atcgcccaac
      901 catggtgaga tagccaaggt gggagaaggc aagtatcgag aggactttca gatggatgaa
      961 ggagaggatc ctagcttcct gttccagtca tacctggaaa atgttggagt ccaaatagtc
     1021 agacaaatga ggtcaggaga gagatttctc aagatatggt cacagaccgt agaagagatt
     1081 atatcctatg tcgcggtcaa ctttcccaac cctccaggaa agtcttcaga ggataaatca
     1141 acccagacta ctggccgaga gctcaagaag gagacaacac ccactccttc tcagagagaa
     1201 agccaatcat cgaaagccag gatggcggct caaattgctt ctggccctcc agcccttgaa
     1261 tggtcggcta ccaatgaaga ggatgatcta tcagtggagg ctgagatcgc tcaccagatt
     1321 gcagaaagtt tctccaaaaa atataagttt ccctctcgat cctcagggat actcttgtat
     1381 aattttgagc aattgaaaat gaaccttgat gatatagtta aagaggcaaa aaatgtacca
     1441 ggtgtgaccc gtttagccca tgacgggtcc aaactccccc taagatgtgt actgggatgg
     1501 gtcgctttgg ccaactctaa gaaattccag ttgttagtcg aatccgacaa gctgagtaaa
     1561 atcatgcaag atgacttgaa tcgctataca tcttgctaac cgaacctctc ccctcagtcc
     1621 ctctagatcg aattcctgca gcccggggga tccggctgct aacaaagccc gaaaggaagc
     1681 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     1741 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatccacta gttctagagc
     1801 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     1861 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2701 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4021 cacctg
//
