LOCUS       V002628                 4535 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002628
VERSION     V002628
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Finke S, Conzelmann KK.
  TITLE     Virus promoters determine interference by defective RNAs: selective
            amplification of mini-RNA vectors and rescue from cDNA by a 3'
            copy-back ambisense rabies virus
  JOURNAL   J. Virol. 73 (5), 3818-3825 (1999)
   PUBMED   10196276
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4535)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 4535)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "1999"; volume: "73"; issue: "5"; pages: "3818-3825"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..164
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        171..189
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    240..701
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             706..2055
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Rabies virus (strain SAD B19).
                     Accession#: P16285"
     terminator      2221..2268
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(2291..2307)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2344..2362)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2383..2399)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2407..2423)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2431..2461)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2476..2497)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2785..3373)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3547..4404)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4405..4509)
                     /label="AmpR promoter"
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      181 cactataggg cgaattaatt ccggttattt tccaccatat tgccgtcttt tggcaatgtg
      241 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      301 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      361 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      421 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      481 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      541 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      601 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
      661 accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatgga tgccgacaag
      721 attgtattca aagtcaataa tcaggtggtc tctttgaagc ctgagattat cgtggatcaa
      781 tatgagtaca agtaccctgc catcaaagat ttgaaaaagc cctgtataac cctaggaaag
      841 gctcccgatt taaataaagc atacaagtca gttttgtcag gcatgagcgc cgccaaactt
      901 aatcctgacg atgtatgttc ctatttggca gcggcaatgc agttttttga ggggacatgt
      961 ccggaagact ggaccagcta tggaattgtg attgcacgaa aaggagataa gatcacccca
     1021 ggttctctgg tggagataaa acgtactgat gtagaaggga attgggctct gacaggaggc
     1081 atggaactga caagagaccc cactgtccct gagcatgcgt ccttagtcgg tcttctcttg
     1141 agtctgtata ggttgagcaa aatatccggg caaaacactg gtaactataa gacaaacatt
     1201 gcagacagga tagagcagat ttttgagaca gccccttttg ttaaaatcgt ggaacaccat
     1261 actctaatga caactcacaa aatgtgtgct aattggagta ctataccaaa cttcagattt
     1321 ttggccggaa cctatgacat gtttttctcc cggattgagc atctatattc agcaatcaga
     1381 gtgggcacag ttgtcactgc ttatgaagac tgttcaggac tggtatcatt tactgggttc
     1441 ataaaacaaa tcaatctcac cgctagagag gcaatactat atttcttcca caagaacttt
     1501 gaggaagaga taagaagaat gtttgagcca gggcaggaga cagctgttcc tcactcttat
     1561 ttcatccact tccgttcact aggcttgagt gggaaatctc cttattcatc aaatgctgtt
     1621 ggtcacgtgt tcaatctcat tcactttgta ggatgctata tgggtcaagt cagatcccta
     1681 aatgcaacgg ttattgctgc atgtgctcct catgaaatgt ctgttctagg gggctatctg
     1741 ggagaggaat tcttcgggaa agggacattt gaaagaagat tcttcagaga tgagaaagaa
     1801 cttcaagaat acgaggcggc tgaactgaca aagactgacg tagcactggc agatgatgga
     1861 actgtcaact ctgacgacga ggactacttt tcaggtgaaa ccagaagtcc ggaggctgtt
     1921 tatactcgaa tcatgatgaa tggaggtcga ctaaagagat ctcacatacg gagatatgtc
     1981 tcagtcagtt ccaatcatca agcccgtcca aactcattcg ccgagtttct aaacaagaca
     2041 tattcgagtg actcataaga agttgaataa caaaatgccg gaaatctacg gattgtgtat
     2101 atccatcatg aaaaaaacta acacccctcc tttcgatcga attcctgcag cccgggggat
     2161 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     2221 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     2281 actatatccg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     2341 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     2401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4501 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
