LOCUS       V002629                 9575 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002629
VERSION     V002629
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Finke S, Conzelmann KK.
  TITLE     Virus promoters determine interference by defective RNAs: selective
            amplification of mini-RNA vectors and rescue from cDNA by a 3'
            copy-back ambisense rabies virus
  JOURNAL   J. Virol. 73 (5), 3818-3825 (1999)
   PUBMED   10196276
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9575)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 9575)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "1999"; volume: "73"; issue: "5"; pages: "3818-3825"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..164
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        171..189
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    240..701
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             706..7086
                     /gene="L"
                     /label="Large structural protein"
                     /note="Large structural protein from Rabies virus (strain
                     SAD B19). Accession#: P16289"
     terminator      7261..7308
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(7331..7347)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(7384..7402)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7423..7439)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7447..7463)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7471..7501)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7516..7537)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7825..8413)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8587..9444)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9445..9549)
                     /label="AmpR promoter"
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      181 cactataggg cgaattaatt ccggttattt tccaccatat tgccgtcttt tggcaatgtg
      241 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      301 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      361 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      421 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      481 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      541 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      601 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
      661 accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatgct cgatcctgga
      721 gaggtctatg atgaccctat tgacccaatc gagttagagg ctgaacccag aggaaccccc
      781 attgtcccca acatcttgag gaactctgac tacaatctca actctccttt gatagaagat
      841 cctgctagac taatgttaga atggttaaaa acagggaata gaccttatcg gatgactcta
      901 acagacaatt gctccaggtc tttcagagtt ttgaaagatt atttcaagaa ggtagatttg
      961 ggttctctca aggtgggcgg aatggctgca cagtcaatga tttctctctg gttatatggt
     1021 gcccactctg aatccaacag gagccggaga tgtataacag acttggccca tttctattcc
     1081 aagtcgtccc ccatagagaa gctgttgaat ctcacgctag gaaatagagg gctgagaatc
     1141 cccccagagg gagtgttaag ttgccttgag agggttgatt atgataatgc atttggaagg
     1201 tatcttgcca acacgtattc ctcttacttg ttcttccatg taatcacctt atacatgaac
     1261 gccctagact gggatgaaga aaagaccatc ctagcattat ggaaagattt aacctcagtg
     1321 gacatcggga aggacttggt aaagttcaaa gaccaaatat ggggactgct gatcgtgaca
     1381 aaggactttg tttactccca aagttccaat tgtctttttg acagaaacta cacacttatg
     1441 ctaaaagatc ttttcttgtc tcgcttcaac tccttaatgg tcttgctctc tcccccagag
     1501 ccccgatact cagatgactt gatatctcaa ctatgccagc tgtacattgc tggggatcaa
     1561 gtcttgtcta tgtgtggaaa ctccggctat gaagtcatca aaatattgga gccatatgtc
     1621 gtgaatagtt tagtccagag agcagaaaag tttaggcctc tcattcattc cttgggagac
     1681 tttcctgtat ttataaaaga caaggtaagt caacttgaag agacgttcgg tccctgtgca
     1741 agaaggttct ttagggctct ggatcaattc gacaacatac atgacttggt ttttgtgttt
     1801 ggctgttaca ggcattgggg gcacccatat atagattatc gaaagggtct gtcaaaacta
     1861 tatgatcagg ttcaccttaa aaaaatgata gataagtcct accaggagtg cttagcaagc
     1921 gacctagcca ggaggatcct tagatggggt tttgataagt actccaagtg gtatctggat
     1981 tcaagattcc tagcccgaga ccaccccttg actccttata tcaaaaccca aacatggcca
     2041 cccaaacata ttgtagactt ggtgggggat acatggcaca agctcccgat cacgcagatc
     2101 tttgagattc ctgaatcaat ggatccgtca gaaatattgg atgacaaatc acattctttc
     2161 accagaacga gactagcttc ttggctgtca gaaaaccgag gggggcctgt tcctagcgaa
     2221 aaagttatta tcacggccct gtctaagccg cctgtcaatc cccgagagtt tctgaggtct
     2281 atagacctcg gaggattgcc agatgaagac ttgataattg gcctcaagcc aaaggaacgg
     2341 gaattgaaga ttgaaggtcg attctttgct ctaatgtcat ggaatctaag attgtatttt
     2401 gtcatcactg aaaaactctt ggccaactac atcttgccac tttttgacgc gctgactatg
     2461 acagacaacc tgaacaaggt gtttaaaaag ctgatcgaca gggtcaccgg gcaagggctt
     2521 ttggactatt caagggtcac atatgcattt cacctggact atgaaaagtg gaacaaccat
     2581 caaagattag agtcaacaga ggatgtattt tctgtcctag atcaagtgtt tggattgaag
     2641 agagtgtttt ctagaacaca cgagtttttt caaaaggcct ggatctatta ttcagacaga
     2701 tcagacctca tcgggttacg ggaggatcaa atatactgct tagatgcgtc caacggccca
     2761 acctgttgga atggccagga tggcgggcta gaaggcttac ggcagaaggg ctggagtcta
     2821 gtcagcttat tgatgataga tagagaatct caaatcagga acacaagaac caaaatacta
     2881 gctcaaggag acaaccaggt tttatgtccg acatacatgt tgtcgccagg gctatctcaa
     2941 gaggggctcc tctatgaatt ggagagaata tcaaggaatg cactttcgat atacagagcc
     3001 gtcgaggaag gggcatctaa gctagggctg atcatcaaga aagaagagac catgtgtagt
     3061 tatgacttcc tcatctatgg aaaaacccct ttgtttagag gtaacatatt ggtgcctgag
     3121 tccaaaagat gggccagagt ctcttgcgtc tctaatgacc aaatagtcaa cctcgccaat
     3181 ataatgtcga cagtgtccac caatgcgcta acagtggcac aacactctca atctttgatc
     3241 aaaccgatga gggattttct gctcatgtca gtacaggcag tctttcacta cctgctattt
     3301 agcccaatct taaagggaag agtttacaag attctgagcg ctgaagggga gagctttctc
     3361 ctagccatgt caaggataat ctatctagat ccttctttgg gagggatatc tggaatgtcc
     3421 ctcggaagat tccatatacg acagttctca gaccctgtct ctgaagggtt atccttctgg
     3481 agagagatct ggttaagctc ccaagagtcc tggattcacg cgttgtgtca agaggctgga
     3541 aacccagatc ttggagagag aacactcgag agcttcactc gccttctaga agatccgacc
     3601 accttaaata tcagaggagg ggccagtcct accattctac tcaaggatgc aatcagaaag
     3661 gctttatatg acgaggtgga caaggtggaa aattcagagt ttcgagaggc aatcctgttg
     3721 tccaagaccc atagagataa ttttatactc ttcttaatat ctgttgagcc tctgtttcct
     3781 cgatttctca gtgagctatt cagttcgtct tttttgggaa tccccgagtc aatcattgga
     3841 ttgatacaaa actcccgaac gataagaagg cagtttagaa agagtctctc aaaaacttta
     3901 gaagaatcct tctacaactc agagatccac gggattagtc ggatgaccca gacacctcag
     3961 agggttgggg gggtgtggcc ttgctcttca gagagggcag atctacttag ggagatctct
     4021 tggggaagaa aagtggtagg cacgacagtt cctcaccctt ctgagatgtt gggattactt
     4081 cccaagtcct ctatttcttg cacttgtgga gcaacaggag gaggcaatcc tagagtttct
     4141 gtatcagtac tcccgtcctt tgatcagtca tttttttcac gaggccccct aaagggatac
     4201 ttgggctcgt ccacctctat gtcgacccag ctattccatg catgggaaaa agtcactaat
     4261 gttcatgtgg tgaagagagc tctatcgtta aaagaatcta taaactggtt cattactaga
     4321 gattccaact tggctcaagc tctaattagg aacattatgt ctctgacagg ccctgatttc
     4381 cctctagagg aggcccctgt cttcaaaagg acggggtcag ccttgcatag gttcaagtct
     4441 gccagataca gcgaaggagg gtattcttct gtctgcccga acctcctctc tcatatttct
     4501 gttagtacag acaccatgtc tgatttgacc caagacggga agaactacga tttcatgttc
     4561 cagccattga tgctttatgc acagacatgg acatcagagc tggtacagag agacacaagg
     4621 ctaagagact ctacgtttca ttggcacctc cgatgcaaca ggtgtgtgag acccattgac
     4681 gacgtgaccc tggagacctc tcagatcttc gagtttccgg atgtgtcgaa aagaatatcc
     4741 agaatggttt ctggggctgt gcctcacttc cagaggcttc ccgatatccg tctgagacca
     4801 ggagattttg aatctctaag cggtagagaa aagtctcacc atatcggatc agctcagggg
     4861 ctcttatact caatcttagt ggcaattcac gactcaggat acaatgatgg aaccatcttc
     4921 cctgtcaaca tatacggcaa ggtttcccct agagactatt tgagagggct cgcaagggga
     4981 gtattgatag gatcctcgat ttgcttcttg acaagaatga caaatatcaa tattaataga
     5041 cctcttgaat tggtctcagg ggtaatctca tatattctcc tgaggctaga taaccatccc
     5101 tccttgtaca taatgctcag agaaccgtct cttagaggag agatattttc tatccctcag
     5161 aaaatccccg ccgcttatcc aaccactatg aaagaaggca acagatcaat cttgtgttat
     5221 ctccaacatg tgctacgcta tgagcgagag ataatcacgg cgtctccaga gaatgactgg
     5281 ctatggatct tttcagactt tagaagtgcc aaaatgacgt acctatccct cattacttac
     5341 cagtctcatc ttctactcca gagggttgag agaaacctat ctaagagtat gagagataac
     5401 ctgcgacaat tgagttcttt gatgaggcag gtgctgggcg ggcacggaga agatacctta
     5461 gagtcagacg acaacattca acgactgcta aaagactctt tacgaaggac aagatgggtg
     5521 gatcaagagg tgcgccatgc agctagaacc atgactggag attacagccc caacaagaag
     5581 gtgtcccgta aggtaggatg ttcagaatgg gtctgctctg ctcaacaggt tgcagtctct
     5641 acctcagcaa acccggcccc tgtctcggag cttgacataa gggccctctc taagaggttc
     5701 cagaaccctt tgatctcggg cttgagagtg gttcagtggg caaccggtgc tcattataag
     5761 cttaagccta ttctagatga tctcaatgtt ttcccatctc tctgccttgt agttggggac
     5821 gggtcagggg ggatatcaag ggcagtcctc aacatgtttc cagatgccaa gcttgtgttc
     5881 aacagtcttt tagaggtgaa tgacctgatg gcttccggaa cacatccact gcctccttca
     5941 gcaatcatga ggggaggaaa tgatatcgtc tccagagtga tagatcttga ctcaatctgg
     6001 gaaaaaccgt ccgacttgag aaacttggca acctggaaat acttccagtc agtccaaaag
     6061 caggtcaaca tgtcctatga cctcattatt tgcgatgcag aagttactga cattgcatct
     6121 atcaaccgga tcaccctgtt aatgtccgat tttgcattgt ctatagatgg accactctat
     6181 ttggtcttca aaacttatgg gactatgcta gtaaatccaa actacaaggc tattcaacac
     6241 ctgtcaagag cgttcccctc ggtcacaggg tttatcaccc aagtaacttc gtctttttca
     6301 tctgagctct acctccgatt ctccaaacga gggaagtttt tcagagatgc tgagtacttg
     6361 acctcttcca cccttcgaga aatgagcctt gtgttattca attgtagcag ccccaagagt
     6421 gagatgcaga gagctcgttc cttgaactat caggatcttg tgagaggatt tcctgaagaa
     6481 atcatatcaa atccttacaa tgagatgatc ataactctga ttgacagtga tgtagaatct
     6541 tttctagtcc acaagatggt tgatgatctt gagttacaga ggggaactct gtctaaagtg
     6601 gctatcatta tagccatcat gatagttttc tccaacagag tcttcaacgt ttccaaaccc
     6661 ctaactgacc cctcgttcta tccaccgtct gatcccaaaa tcctgaggca cttcaacata
     6721 tgttgcagta ctatgatgta tctatctact gctttaggtg acgtccctag cttcgcaaga
     6781 cttcacgacc tgtataacag acctataact tattacttca gaaagcaagt cattcgaggg
     6841 aacgtttatc tatcttggag ttggtccaac gacacctcag tgttcaaaag ggtagcctgt
     6901 aattctagcc tgagtctgtc atctcactgg atcaggttga tttacaagat agtgaagact
     6961 accagactcg ttggcagcat caaggatcta tccagagaag tggaaagaca ccttcatagg
     7021 tacaacaggt ggatcaccct agaggatatc agatctagat catccctact agactacagt
     7081 tgcctgtgaa ccggatactc ctggaagcct gcccatgcta agactcttgt gtgatgtatc
     7141 ttgaaaaaaa caagatccta aatctgaacc tttggatcga attcctgcag cccgggggat
     7201 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     7261 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     7321 actatatccg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     7381 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7441 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7501 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7561 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7621 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7681 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7741 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7801 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7861 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7921 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7981 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8041 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8101 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8161 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8221 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8281 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8341 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8401 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8821 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8881 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8941 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9001 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9061 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9121 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9181 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9241 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9301 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9361 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9421 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9481 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9541 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
