LOCUS       V002630                 4932 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002630
VERSION     V002630
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Finke S, Mueller-Waldeck R, Conzelmann KK.
  TITLE     Rabies virus matrix protein regulates the balance of virus
            transcription and replication
  JOURNAL   J. Gen. Virol. 84 (PT 6), 1613-1621 (2003)
   PUBMED   12771432
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4932)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 4932)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Gen.
            Virol. 84 (PT 6), 1613-1621 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..164
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        171..189
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    240..701
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             706..2277
                     /gene="G"
                     /label="Glycoprotein"
                     /note="Glycoprotein from Rabies virus (strain SAD B19).
                     Accession#: P16288"
     terminator      2618..2665
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(2688..2704)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2741..2759)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2780..2796)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2804..2820)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2828..2858)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2873..2894)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3182..3770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3944..4801)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4802..4906)
                     /label="AmpR promoter"
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      181 cactataggg cgaattaatt ccggttattt tccaccatat tgccgtcttt tggcaatgtg
      241 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      301 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      361 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      421 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      481 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      541 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      601 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
      661 accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatggt tcctcaggct
      721 ctcctgtttg taccccttct ggtttttcca ttgtgttttg ggaaattccc tatttacacg
      781 ataccagaca agcttggtcc ctggagtccg attgacatac atcacctcag ctgcccaaac
      841 aatttggtag tggaggacga aggatgcacc aacctgtcag ggttctccta catggaactt
      901 aaagttggat acatcttagc cataaaagtg aacgggttca cttgcacagg cgttgtgacg
      961 gaggctgaaa cctacactaa cttcgttggt tatgtcacaa ccacgttcaa aagaaagcat
     1021 ttccgcccaa caccagatgc atgtagagcc gcgtacaact ggaagatggc cggtgacccc
     1081 agatatgaag agtctctaca caatccgtac cctgactacc gctggcttcg aactgtaaaa
     1141 accaccaagg agtctctcgt tatcatatct ccaagtgtgg cagatttgga cccatatgac
     1201 agatcccttc actcgagggt cttccctagc gggaagtgct caggagtagc ggtgtcttct
     1261 acctactgct ccactaacca cgattacacc atttggatgc ccgagaatcc gagactaggg
     1321 atgtcttgtg acatttttac caatagtaga gggaagagag catccaaagg gagtgagact
     1381 tgcggctttg tagatgaaag aggcctatat aagtctttaa aaggagcatg caaactcaag
     1441 ttatgtggag ttctaggact tagacttatg gatggaacat gggtctcgat gcaaacatca
     1501 aatgaaacca aatggtgccc tcccgataag ttggtgaacc tgcacgactt tcgctcagac
     1561 gaaattgagc accttgttgt agaggagttg gtcaggaaga gagaggagtg tctggatgca
     1621 ctagagtcca tcatgacaac caagtcagtg agtttcagac gtctcagtca tttaagaaaa
     1681 cttgtccctg ggtttggaaa agcatatacc atattcaaca agaccttgat ggaagccgat
     1741 gctcactaca agtcagtcag aacttggaat gagatcctcc cttcaaaagg gtgtttaaga
     1801 gttgggggga ggtgtcatcc tcatgtgaac ggggtgtttt tcaatggtat aatattagga
     1861 cctgacggca atgtcttaat cccagagatg caatcatccc tcctccagca acatatggag
     1921 ttgttggaat cctcggttat cccccttgtg caccccctgg cagacccgtc taccgttttc
     1981 aaggacggtg acgaggctga ggattttgtt gaagttcacc ttcccgatgt gcacaatcag
     2041 gtctcaggag ttgacttggg tctcccgaac tgggggaagt atgtattact gagtgcaggg
     2101 gccctgactg ccttgatgtt gataattttc ctgatgacat gttgtagaag agtcaatcga
     2161 tcagaaccta cgcaacacaa tctcagaggg acagggaggg aggtgtcagt cactccccaa
     2221 agcgggaaga tcatatcttc atgggaatca cacaagagtg ggggtgagac cagactgtaa
     2281 ggactggccg tcctttcaac gatccaagtc ctgaagatca cctccccttg gggggttctt
     2341 tttgaaaaac ctgggttcaa tagtcctcct tgaactccat gcaactgggt agattcaaga
     2401 gtcatgagat tttcattaat cctctcagtt gatcaagcaa gatcatgtcg attctcataa
     2461 taggggagat cttctagcag tttcagtggc taacggtact ttcattctcc aggaactgac
     2521 accaacactc gtgccgaatt cctgcagccc gggggatccg gctgctaaca aagcccgaaa
     2581 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     2641 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat ccactagttc
     2701 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
     2761 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2821 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2881 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2941 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3001 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3061 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4921 aagtgccacc tg
//
