LOCUS       V002648                10275 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002648
VERSION     V002648
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA.
  TITLE     Engineered promoters enable constant gene expression at any copy
            number in bacteria
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   29553576
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 10275)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..12
                     /label="GGAG seam"
                     /note="GGAG seam"
     regulatory      13..73
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      78..146
                     /label="PT7A1w3"
                     /note="PT7A1w3"
                     /regulatory_class="promoter"
     ncRNA           147..225
                     /product="SarJ"
                     /label="ribozyme"
                     /note="insulator"
                     /ncRNA_class="ribozyme"
     regulatory      226..245
                     /label="RBSsp2"
                     /note="RBSsp2"
                     /regulatory_class="ribosome_binding_site"
     CDS             246..2885
                     /codon_start=1
                     /product="TALEsp2"
                     /label="TALEsp2"
                     /protein_id="AVR55202.1"
                     /translation="MVDLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVAL
                     SQHPAALGTVAVKYQDMIAALPEATHEAIVGVGKQWSGARALEALLTVAGELRGPPLQL
                     DTGQLLKIAKRGGVTAVEAVHAWRNALTGAPLNLTPDQVVAIASHDGGKQALETVQRLL
                     PVLCQDHGLTPEQVVAIASHDGGKQALETVQRLLPVLCQAHGLTPDQVVAIASNGGGKQ
                     ALETVQRLLPVLCQAHGLTPAQVVAIASNIGGKQALETVQRLLPVLCQDHGLTPDQVVA
                     IASNGGGKQALETVQRLLPVLCQDHGLTPEQVVAIASNIGGKQALETVQRLLPVLCQAH
                     GLTPDQVVAIASHDGGKQALETVQRLLPVLCQAHGLTPAQVVAIASNNGGKQALETVQR
                     LLPVLCQDHGLTPDQVVAIASNIGGKQALETVQRLLPVLCQDHGLTPEQVVAIASHDGG
                     KQALETVQRLLPVLCQAHGLTPDQVVAIASNGGGKQALETVQRLLPVLCQAHGLTPAQV
                     VAIASHDGGKQALETVQRLLPVLCQDHGLTPDQVVAIASNIGGKQALETVQRLLPVLCQ
                     DHGLTPEQVVAIASNIGGKQALETVQRLLPVLCQAHGLTPDQVVAIASNGGGKQALETV
                     QRLLPVLCQAHGLTPAQVVAIASNIGGKQALETVQRLLPVLCQDHGLTPEQVVAIASNG
                     GGRPALESIVAQLSRPDPALAALTNDHLVALACLGGRPALDAVKKGLPHAPALIKRTNR
                     RIPERTSHRVADHAQVVRVLGFFQCHSHPAQAFDDAMTQFGMSRHGLLQLFRRVGVTEL
                     EARSGTLPPASQRWDRILQASGMKRAKPSPTSTQTPDQASLHAFADSLERDLDAPSPMH
                     EGDQTRAS"
     regulatory      complement(2891..2980)
                     /label="ECK120029600"
                     /note="ECK120029600"
                     /regulatory_class="terminator"
     regulatory      2985..3059
                     /label="PUPsp2"
                     /note="PUPsp2"
                     /regulatory_class="promoter"
     misc_feature    3010..3016
                     /label="bfpT7A1"
                     /note="bfpT7A1"
     protein_bind    complement(3018..3035)
                     /label="TALEsp2 binding site"
                     /bound_moiety="TALEsp2"
     misc_RNA        3061..3111
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      3135..3157
                     /label="RBSVioEs"
                     /note="RBSVioEs"
                     /regulatory_class="ribosome_binding_site"
     CDS             3158..3733
                     /codon_start=1
                     /product="VioE"
                     /label="VioE"
                     /protein_id="AVR55201.1"
                     /translation="MENREPPLLPARWSSAYVSYWSPMLPDDQLTSGYCWFDYERDICR
                     IDGLFNPWSERDTGYRLWMSEVGNAASGRTWKQKVAYGRERTALGEQLCERPLDDETGP
                     FAELFLPRDVLRRLGARHIGRRVVLGREADGWRYQRPGKGPSTLYLDAASGTPLRMVTG
                     DEASRASLRDFPNVSEAEIPDAVFAAKR"
     regulatory      3734..3757
                     /label="RBSVioAs"
                     /note="RBSVioAs"
                     /regulatory_class="ribosome_binding_site"
     CDS             3758..5011
                     /gene="vioA"
                     /label="Flavin-dependent L-tryptophan oxidase VioA"
                     /note="Flavin-dependent L-tryptophan oxidase VioA from
                     Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 /
                     JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757).
                     Accession#: Q9S3V1"
     regulatory      5015..5038
                     /label="RBSVioBs"
                     /note="RBSVioBs"
                     /regulatory_class="ribosome_binding_site"
     CDS             5039..8032
                     /gene="vioB"
                     /label="2-imino-3-(indol-3-yl)propanoate dimerase"
                     /note="2-imino-3-(indol-3-yl)propanoate dimerase from
                     Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 /
                     JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757).
                     Accession#: Q9S3V0"
     terminator      8050..8121
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8137..8164
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    8183..8203
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      8204..8261
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      8433..9021
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9222..10013)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      complement(10208..10251)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    10254..10275
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 gaagacacgg agctcggtac caaattccag aaaagaggcc tcccgaaagg ggggcctttt
       61 ttcgttttgg tcctactggc gcgcctcagt cagagtattg acttaaagtc taacctatag
      121 gagatctaca gccatcgaga gctgcgagac tgtcgccgga tgtgtatccg acctgacgat
      181 ggcccaaaag ggccgaaaca gtcctctaca aataattttg tttaaatcaa ttcatcgacg
      241 tgaaaatggt agatttaaga actttaggat attcacagca gcaacaggaa aagatcaagc
      301 ccaaagttag gtcgacagtc gcgcagcatc acgaagcgct ggttggtcat gggtttacac
      361 atgcccacat cgtagcctta tcgcagcacc ctgcagccct tggcacggtc gccgtcaagt
      421 accaggacat gattgcggcg ttgccggaag ccacacatga ggcgatcgtc ggtgtgggga
      481 aacagtggag cggagcccga gcgcttgagg ccctgttgac ggtcgcggga gagctgagag
      541 ggcctcccct tcagctggac acgggccagt tgctgaagat cgcgaagcgg ggaggagtca
      601 cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac gggagcaccc ctcaacctga
      661 ccccggacca ggtagtcgcg attgcttcac atgacggggg taaacaagcg ctggaaacgg
      721 tgcagcgtct gctaccggtg ttatgtcagg atcatgggct cacgccggaa caggtagtgg
      781 caattgcgag tcatgacggt ggcaaacagg ccctggaaac cgtacagcgg ctgctaccgg
      841 tgctgtgtca agcgcatggc ctgactccgg accaagttgt agccattgcc tcgaacgggg
      901 gcggcaagca ggcgctggag actgttcaac gtctgctccc cgttctgtgt caggcgcatg
      961 gcctgacgcc tgcgcaggtc gtggcgatcg cttcaaacat cggtgggaag caagccctgg
     1021 agactgtcca aagactgttg ccagtgttgt gtcaagatca tggcttaacc ccagatcagg
     1081 tggttgcgat tgcatcaaat ggaggtggta aacaggcgct ggagactgtg cagcgcctgt
     1141 tgccggttct gtgccaagat catgggctga ctccggaaca ggttgtggct atcgcaagca
     1201 atattggtgg caagcaggcc ctggaaacag tacagcgcct gctgcctgta ttgtgtcaag
     1261 cccacggtct tacccccgat caggtagtcg ccatagcatc gcacgacggc gggaagcagg
     1321 cccttgagac tgtacaacgc ctcctgccgg ttttgtgcca ggcgcacggc ctgacgccag
     1381 cccaggtggt tgcgatagcc agtaataacg gcggtaagca agcccttgaa accgttcaac
     1441 gtttgctgcc agtgctgtgc caggatcacg gcctgacccc ggatcaggta gtcgccattg
     1501 ctagcaacat tggtggcaaa caagcactgg agacagttca acgcttactg cccgtgcttt
     1561 gccaggatca tggactgacc ccagagcaag tggtcgcgat tgcctcgcat gacggaggta
     1621 aacaggccct tgagactgtc cagcgtctgc tgccggtcct ttgccaggct catgggctga
     1681 cgcccgacca ggtggtagca atcgcttcga acggcggagg caaacaggcg ttagaaaccg
     1741 ttcaacgtct gttaccggtg ctgtgccaag ctcatggctt aaccccggcc caggtagtcg
     1801 caatcgctag tcatgatggt gggaaacagg cattagaaac agtacaacgt ctgctcccgg
     1861 tcctctgtca ggatcacggt ctgaccccgg atcaggttgt agcaatcgct agcaatattg
     1921 gtggtaaaca ggcgctggaa acagtgcaaa gattattacc agttctgtgt caggaccatg
     1981 gcctgacacc tgagcaggtc gtagcgatcg caagtaatat tggaggtaaa caggccctgg
     2041 aaaccgtgca gcgtctgctg cctgtgctct gtcaagcgca tggtctgact ccggatcagg
     2101 ttgtcgcgat cgccagtaac gggggaggga aacaggcact cgagactgtg cagagattgc
     2161 tgccggtcct gtgtcaggcg catggtctga ccccagcgca ggtcgtggca atcgcttcca
     2221 acataggtgg taaacaggcc ctcgaaactg tccaacggct gttaccggta ctgtgccagg
     2281 atcatggtct gacccctgag caggtagtgg ctattgcatc caacggaggg ggcagacccg
     2341 cactggagtc aatcgtggcc cagctttcga ggccggaccc cgcgctggcc gcactcacta
     2401 atgatcatct tgtagcgctg gcctgcctcg gcggacgacc cgccttggat gcggtgaaga
     2461 aggggctccc gcacgcgcct gcattgatta agcggaccaa cagaaggatt cccgagagga
     2521 catcacatcg agtggcagat cacgcgcaag tggtccgcgt gctcggattc ttccagtgtc
     2581 actcccaccc cgcacaagcg ttcgatgacg ccatgactca atttggtatg tcgagacacg
     2641 gactgctgca gctctttcgt agagtcggtg tcacagaact cgaggcccgc tcgggcacac
     2701 tgcctcccgc ctcccagcgg tgggacagga ttctccaagc gagcggtatg aaacgcgcga
     2761 agccttcacc tacgtcaact cagacacctg accaggcgag ccttcatgcg ttcgcagact
     2821 cgctggagag ggatttggac gcgccctcgc ccatgcatga aggggaccaa actcgcgcgt
     2881 cataataggt ttcagccaaa aaacttaaga ccgccggtct tgtccactac cttgcagtaa
     2941 tgcggtggac aggatcggcg gttttctttt ctcttctcaa gcttatcccg aaaatttatc
     3001 aaaaagagta ttgacttata ttgagtcgta taggatactt acagccatcg agagctgcga
     3061 gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa
     3121 ataattttgt ttaatactca ttagggaggg aaaaaaaatg gagaaccgtg agccaccact
     3181 gttgccagcc cgttggagca gcgcctatgt ctcttattgg agcccgatgc tgccggatga
     3241 ccagctgacc agcggctatt gctggttcga ctatgaacgt gacatctgtc gtattgacgg
     3301 cctgttcaat ccgtggagcg agcgtgatac tggttatcgc ctgtggatgt cggaggttgg
     3361 taatgcggcc agcggccgta cctggaaaca aaaagtcgcc tatggtcgtg agcgtaccgc
     3421 cctgggtgaa cagctgtgtg agcgtccgct ggatgatgag actggccctt ttgccgaatt
     3481 gttcctgcca cgcgatgtcc tgcgccgtct gggtgcccgt cacattggcc gtcgcgtggt
     3541 tctgggtcgc gaagcggacg gttggcgtta ccagcgccca ggtaaaggtc cgagcaccct
     3601 gtacctggat gcggcgagcg gcactccact gcgcatggtc accggcgatg aagcgtcgcg
     3661 tgcaagcctg cgtgattttc cgaatgtgag cgaggcggag atcccggacg cggttttcgc
     3721 ggccaagcgc taataacatt atagggtggg aaaaaaaatg aaacattctt ccgatatctg
     3781 cattgttggt gctggtattt ctggtttgac gtgcgcaagc catctgctgg acagcccggc
     3841 atgccgtggt ctgagcctgc gtatctttga catgcagcaa gaagccggtg gccgtatccg
     3901 cagcaaaatg ctggatggta aggcaagcat tgaactgggc gcaggtcgct actcccctca
     3961 gttgcacccg catttccaaa gcgcaatgca gcactatagc caaaagagcg aagtctatcc
     4021 gttcacccag ttgaagttca aatctcacgt gcagcaaaag ctgaagcgcg ccatgaatga
     4081 actgtccccg cgtctgaaag agcatggtaa agagagcttt ttgcagtttg tcagccgtta
     4141 tcaaggtcac gatagcgcgg ttggtatgat ccgctctatg ggttacgacg cactgttcct
     4201 gccggatatc agcgcagaaa tggcctacga cattgtgggt aagcacccgg agatccagag
     4261 cgtgacggac aacgacgcga accaatggtt tgcagcggaa acgggctttg ctggtctgat
     4321 tcagggcatc aaggctaagg ttaaggcggc aggtgcgcgt tttagcctgg gttatcgtct
     4381 gctgagcgtc cgtaccgacg gtgacggcta cctgctgcaa ctggcaggtg acgacggctg
     4441 gaaactggag caccgtaccc gccatctgat tctggcgatt ccgccgagcg cgatggcggg
     4501 tttgaatgtt gattttccag aagcctggtc cggtgcgcgc tatggcagcc tgccgctgtt
     4561 taagggcttt ctgacgtacg gtgagccgtg gtggttggac tacaaactgg acgatcaggt
     4621 gctgattgtt gacaacccgc tgcgcaaaat ctatttcaaa ggcgataagt acctgttctt
     4681 ctataccgat agcgagatgg cgaattactg gcgcggttgt gtcgcggagg gcgaggacgg
     4741 ttacctggag caaattcgca cccatttggc tagcgcactg ggtatcgtcc gtgaacgtat
     4801 cccgcaaccg ctggcacacg ttcacaagta ttgggcgcac ggcgttgagt tttgccgtga
     4861 ttctgatatt gaccacccga gcgcactgtc tcatcgcgac agcggtatca tcgcgtgctc
     4921 cgatgcgtac acggagcatt gtggttggat ggagggcggt ctgctgagcg cccgtgaggc
     4981 aagccgtctg ctgttgcagc gtatcgccgc gtgataggtc attatggggg taaaaaaaat
     5041 gagcattctg gatttcccgc gtatccactt ccgtggctgg gcccgtgtca atgcgccgac
     5101 cgcgaaccgc gatccgcacg gccacatcga tatggccagc aataccgtgg cgatggcggg
     5161 tgagccgttc gacctggcac gccatcctac ggagttccac cgtcacctgc gctccctggg
     5221 tccgcgcttc ggcttggatg gtcgtgctga cccggaaggc ccgttcagcc tggccgaggg
     5281 ctacaacgct gccggtaaca accacttttc gtgggagagc gcaaccgtta gccacgtgca
     5341 atgggatggc ggtgaggcgg atcgtggtga cggtctggtc ggtgctcgtt tggcactgtg
     5401 gggtcactac aatgattatc tgcgtaccac cttcaatcgt gctcgttggg tcgacagcga
     5461 cccgacgcgc cgtgacgctg cacaaatcta tgcgggccaa ttcaccatta gcccggctgg
     5521 tgccggtccg ggtacgccgt ggctgtttac ggcagacatt gatgatagcc atggtgcacg
     5581 ttggacgcgt ggcggccaca ttgcagagcg tggcggccac ttcttggatg aagagtttgg
     5641 tctggcacgc ctgtttcagt tctctgtgcc gaaagatcac ccacattttc tgtttcaccc
     5701 gggtccgttt gattccgagg cctggcgtcg tctgcaattg gctctggagg atgacgacgt
     5761 tctgggtctg accgtgcaat atgcgttgtt caatatgagc accccgcctc agccgaacag
     5821 cccggttttt cacgatatgg tcggtgttgt cggtctgtgg cgtcgtggtg aactggcgag
     5881 ctacccggct ggtcgtctgc tgcgtccgcg tcaaccgggt ctgggtgacc tgaccctgcg
     5941 cgtcaacggt ggtcgcgttg cgctgaattt ggcgtgtgcc attccgttca gcactcgtgc
     6001 cgcgcagcca agcgcaccgg accgcctgac cccggacctg ggtgccaaac tgccgctggg
     6061 cgatctgctg ctgcgtgatg aggacggcgc actgttggca cgtgtgccgc aggctctgta
     6121 ccaagactat tggacgaatc acggtattgt ggacctgccg ctgctgcgcg aaccgcgtgg
     6181 tagcttgacc ctgagcagcg aactggcgga gtggcgtgag caagactggg tcacccaaag
     6241 cgacgcgtct aacctgtacc tggaggcacc ggatcgccgt cacggtcgct ttttccctga
     6301 gagcatcgcg ctgcgcagct actttcgcgg tgaagcgcgt gcgcgtccgg atatcccgca
     6361 tcgtatcgag ggcatgggcc tggtcggcgt cgaatctcgt caggatggcg acgctgcgga
     6421 atggcgtctg acgggtctgc gtccgggtcc ggcacgcatt gttctggacg atggtgccga
     6481 ggcgatccct ctgcgtgttc tgcctgacga ttgggcgctg gatgacgcga ccgtcgaaga
     6541 agtggattac gcctttttgt accgccacgt tatggcgtat tacgagctgg tgtatccatt
     6601 catgagcgac aaggtgtttt ccctggctga tcgttgcaaa tgtgaaacgt acgcacgtct
     6661 gatgtggcag atgtgtgatc cgcagaaccg caacaagtcc tattacatgc cgagcacccg
     6721 cgaactgtcg gcaccgaaag ctcgtttgtt cttgaagtat ctggcccacg tggaaggcca
     6781 ggcacgcctg caagcacctc cgccagcggg tccggcacgc attgaatcta aagcccagtt
     6841 ggcggcagag ctgcgtaaag ccgtcgacct ggagctgtct gtgatgctgc aatacctgta
     6901 cgcggcgtat agcattccga actatgcaca gggccaacaa cgtgttcgtg acggtgcgtg
     6961 gaccgccgag cagctgcaac tggcgtgcgg tagcggtgac cgtcgccgtg atggcggtat
     7021 tcgtgcagca ctgctggaaa ttgctcatga agaaatgatt cattacctgg tcgttaacaa
     7081 cctgctgatg gccctgggcg agccgttcta cgcgggtgtc ccgctgatgg gcgaagcggc
     7141 acgtcaggcg tttggcctgg acaccgagtt cgctctggaa ccgtttagcg aaagcacgct
     7201 ggcacgtttt gttcgtctgg aatggccgca ctttatccca gcaccgggca aatccatcgc
     7261 ggactgctat gccgccattc gtcaggcgtt tttggatctg ccggacttgt ttggtggcga
     7321 ggcaggtaag cgtggcggtg aacaccacct gttcctgaat gagctgacca accgtgcgca
     7381 tccgggttat caactggaag ttttcgatcg cgactcggcg ctgtttggta ttgcatttgt
     7441 gaccgatcag ggcgaaggtg gcgctctgga cagcccgcac tacgaacata gccattttca
     7501 acgtctgcgt gaaatgagcg cgcgtatcat ggctcaaagc gcaccgttcg aaccggcgct
     7561 gccggcgttg cgtaatccgg ttctggatga gagcccgggt tgccaacgtg tcgcagacgg
     7621 tcgtgcgcgt gcgctgatgg cattgtacca aggcgtttat gagctgatgt ttgcgatgat
     7681 ggcgcagcac ttcgccgtga aaccgctggg tagcttgcgt cgcagccgcc tgatgaacgc
     7741 agcaatcgat ctgatgaccg gtctgttgcg tccgctgagc tgcgcgctga tgaacctgcc
     7801 aagcggcatc gccggtcgca cggccggtcc gccgctgccg ggtccggttg acacccgtag
     7861 ctatgacgac tacgcgctgg gctgtcgcat gctggcacgc cgttgcgagc gtctgctgga
     7921 gcaggcgagc atgctggaac cgggttggct gccggatgcg cagatggagc tgctggattt
     7981 ctatcgtcgc caaatgctgg acttggcgtg cggcaaactg agccgcgagg cctaataagg
     8041 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     8101 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     8161 tgcgtttata cgctatgtct tctactagta gcggccgctg cagtccggca aaaaagggca
     8221 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
     8281 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8341 actcaaaggc ggtaattcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9121 cgattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9181 atctaaagta tatatgagta aacttggtct gacagttatc agaagaactc gtcaagaagg
     9241 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     9301 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     9361 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     9421 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     9481 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     9541 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     9601 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     9661 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     9721 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     9781 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     9841 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     9901 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     9961 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
    10021 gatcctcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10141 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10201 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
    10261 cggccgcttc tagag
//
