LOCUS       62056_21190        8251 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Cloning vector pTH1256.1, complete sequence.
ACCESSION   MH550659
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Smith ZJ, Bedore S, Spingler S, Hammond TM.
  TITLE     A mus-51 RIP allele for transformation of Neurospora crassa
  JOURNAL   Fungal Genet Rep 62 (2016)
  PUBMED    28729897
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Hammond TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) School of Biological Sciences, Illinois 
            State University, ISU School of Biological Sciences, Normal, IL 
            61790, USA
REFERENCE   3  (bases 1 to 8251)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Rep 62 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-2018) School of Biological Sciences, Illinois State 
            University, ISU School of Biological Sciences, Normal, IL 61790, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="histone 3"
                     /label=histone 3
                     /note="his-3"
                     /protein_id="QBA09469.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2794..3148
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3153..4175
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(4228..4246)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5605..5616)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(5891..5909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6167..6755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6929..7786)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7787..7891)
                     /label=AmpR promoter
ORIGIN
        1 gaactcgagc agctgaagct tgccatctcc accatcctct cgagcgtctg gaagtctgac
       61 cgtcccgatg gtcttctccc caccgttgtc gttgatgagc acgacactgc tctgggtctg
      121 gtctacagca gtgccgagag tgtgaacgag gccctcagga cacagactgg tgtctatcag
      181 agccggaagc gcggtctctg gtacaagggt gctacttccg gagacactca ggagctcgtc
      241 cgcatctcgc ttgactgcga taacgatgct ctcaagtttg tcgtgaagca gaagggtcgt
      301 ttctgccacc tcgatcagtc cggctgcttt ggtcagctca aaggccttcc caagctcgag
      361 cagactttga tttcgaggaa acagtctgcc cccgagggct cctacactgc ccgtctcttc
      421 tccgatgaga agctagtccg ggccaagatc atggaggagg ctgaggagct ctgcaccgct
      481 cagacccccc aggaaatcgc ctttgaggct gccgatctct tctactttgc tcttaccagg
      541 gccgttgctg ccggcgttac tcttgccgat atcgaaagga gccttgacgc caagagctgg
      601 aaggtcaagc gcaggactgg agatgctaag ggtaagtggg ctgagaagga gggcatcaag
      661 cctgcggcgt ccgctctcgc tgccacttcg gcccctgtca ccaaggaggc cgcccaggag
      721 accacccctg agaagatcac catgagacgt ttcgacgcct ccaaggtctc taccgaggag
      781 ctcgatgctg ctctcaagcg tcctgcgcaa aagtcgtccg atgccatcta caagatcatt
      841 gtccccatca tcgaggacgt ccgcaagaac ggcgacaagg ctgttctgtc gtacactcac
      901 aagttcgaga aggctacctc tcttactagc cccgtcctga aggcgccctt ccccaaggag
      961 cttatgcagc tccctgagga gaccattgct gccatcgacg tgtccttcga gaacatccgc
     1021 aagttccacg ccgcccagaa ggaggagaag cccctccagg tcgagaccat gcccggtgtt
     1081 gtctgcagcc gtttctctcg tcccatcgag gccgtcggct gctacatccc cggcggtacc
     1141 gccgttctcc ccagcactgc ccttatgctg ggtgttcccg ccatggtcgc cggctgcaac
     1201 aagattgtgt tcgcctctcc tccccgcgcc gacggaacca tcactcccga gattgtccac
     1261 gtcgctcaca aggttggggc cgagtccatc gtgcttgccg gcggtgccca ggccgtagct
     1321 gccatggcct acggcaccga gagcatcacc aaggtcgaca agattctcgg ccccggtaac
     1381 cagttcgtca ctgctgccaa gatgttcgtc agcaacgaca ccaacgctgc cgttggtatt
     1441 gacatgcccg ctggcccgtc cgaggtgctg gtcatcgctg acaaggacgc caaccccgcg
     1501 ttcgttgcct cggatctcct gtcccaggct gagcacggcg ttgacagtca ggtcatcctg
     1561 atcgctattg acctcgacga ggagcatctt caggctattg aggacgaggt tcaccgtcag
     1621 gctacggagc ttcctcgcgt ccagattgtc cgtggctcca tcgcccactc gatcaccgtg
     1681 caggtcaaga ccgtcgagga ggccatggag ctcagcaaca agtacgctcc tgagcacttg
     1741 atcctccaga tcaaggaggc cgagaaggct gtcgatcttg tcatgaacgc cggtagtgtc
     1801 ttcattggcg cctggactcc tgagtccgtt ggcgattact ctgctggtgt taaccactcg
     1861 ctgcgtaagt tacatatcat aaatagcccc gcttcacaga ttcttctgct aacgtcaaga
     1921 cacatagcta cctatggctt tggcaagcag tactctggcg tcaatttcgc ctcgttcgtc
     1981 aagcacatta ccagctccaa cttgactgcc gagggtctca aaaacgtcgg ccaggctgtc
     2041 atgcagttgg ctaaggttga ggagctcgag gctcacagaa gggcggtcag catccgtctt
     2101 gagcacatga gcaagagcaa ctaaacggaa attcttttcg aagtagcaaa aaaaaaaaaa
     2161 aaaaacaaga acaaaaggat gtagtgggtt gatgtatatc tgggtcattt tgggcacata
     2221 gagtaatgat aacgagtttt ggacattgta ctgttctgta caggctgaag atcagtacat
     2281 gaatctgttg gtaagtgtgg agacccaaac ggcccttgag tttttctccc tattccagca
     2341 gatgaggtgc tcgtccctgg gtgtttattt tcattattac atcaaccttt tttttttttt
     2401 tttttttcag atcatgcgta catgaacggg ggaagcacag acgatcgaaa cgtggatgtc
     2461 acaatgtccc tgcagtgatg ctgcattgca tgaagcgccc atctcaaaaa acttgcagtc
     2521 ttgcacgttg catgtgaacc tcccaaacaa ccgaataaaa gacggcgaaa aatgaagata
     2581 aaaaaaaacc ataaaaaaaa tcagagggag tgtgggaaat ggtgtctttt agcattcaga
     2641 ccccattagc cgtccacgcc cttaatacga ctcactatag ggcgaattgg agctccaccg
     2701 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
     2761 tatcgatacc gtcgacctcg agggggggcc caactgatat tgaaggagca ttttttgggc
     2821 ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc gtccaggcgg
     2881 tgagcacaaa atttgtgtcg tttgacaaga tggttcattt aggcaactgg tcagatcagc
     2941 cccacttgta gcagtagcgg cggcgctcga agtgtgactc ttattagcag acaggaacga
     3001 ggacattatt atcatctgct gcttggtgca cgataacttg gtgcgtttgt caagcaaggt
     3061 aagtggacga cccggtcata ccttcttaag ttcgcccttc ctccctttat ttcagattca
     3121 atctgactta cctattctac ccaagcatcc aaatgaaaaa gcctgaactc accgcgacgt
     3181 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     3241 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     3301 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     3361 ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt
     3421 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3481 ctgttctcca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3541 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3601 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3661 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3721 aagtccggca cctcgtgcat gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3781 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3841 ccaacatcct cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3901 agcggaggca tccggagctt gcaggatcgc cgcgcctccg ggcgtatatg ctccgcattg
     3961 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     4021 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     4081 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     4141 accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgggaacca
     4201 gttgggcccg gtacccagct tttgttccct ttagtgaggg ttaattaggg ccaagctacc
     4261 ccgtcaaatc tatacatatc tataacgggt ttcaggggtt tcgttcgctg tcaatcaagt
     4321 ttgaaacatc actggggccg ttggacggtg tattagacca ttggctccct cagctgtttg
     4381 gcggctgggc ggctgggtca aacggcaata acgggactcg agagggacga ggagagtcgg
     4441 ttggctggct gcaatacaag cgttcccacc taaccaacga gtcccgtttt ccatttgtgt
     4501 gcctaaccat catctaggga tgtcagggtt tggccggatc agggtatgtt tggttgactg
     4561 ttgtcatgtc tgattgggta catattatgg taggtgtctc gagaacagta gagtactcgg
     4621 gcctagcgtt tggatgatta cgcgagatat gagttgtggg ccgccatgca gttgcttgtc
     4681 cataagcaga agttgctttg ggatatattt ctcgtctttc aaaggtcacg aggtcctggg
     4741 acgaacggca tcgccatcca aagggttgaa catgagaaac ctgaatggcc tttgcgttga
     4801 aatacaaaaa gtcaagaaca aaatcgcttg aggataggga cgtggaagca agcaaatatg
     4861 gtaagagagg tatacatcaa ccctggttca attgttagcg tggttcttcc tccacgtcct
     4921 cgttcatgac ggttaacagt accaggctaa caattaaacc agggttgatg tgtactgata
     4981 tgtaggtgct cagcaaactg ccaatttctt ggccccaagc agcagtttgc tgtcagtgct
     5041 gctcgtgtca gccttggtag tggaacctaa actgctaaca cagcgcaagt gcgcatgtaa
     5101 agatattgtg ggaggatctg tatggatgga tgagattact gcttggtgtt ggttgcgagg
     5161 cactgcggct gttaggcttt gctgtgcccc gttcgacgaa gaaatacgcg gaactataaa
     5221 ttggatacct agacttactg cctatgggag gtatctaccg acgtagccga cggattctag
     5281 caacatcccg actttgcttg tagtgtacta tgatagcagc acagtgttgc tccttgtgag
     5341 aatgggctct tttttttttt cccccttccc tagggcgttg actggacttg ctctattgtt
     5401 cccaaggtag gtgcccgtca tcgattttcc caagtctccc gccagattgt cgtcatagtg
     5461 tcatgatgac ctcggtcgct ggggctgcgt ggttacgggg agctgggacc gctaggccta
     5521 cagtggttgt gccattcagc gtgggtgtgt ggagtagcgg tagaggcgct tggaagttgt
     5581 gctagcggaa accctggaat atcttctacc ctcgattcct tctcgggctg cccatgtgct
     5641 gaggtgatgc cggggatctg gcgccaatca tccattgagg ttcccgcagc ttcccggtgc
     5701 cgcgcgcggg cgcagttgct cacaggacac acctagacgc aggggcacag gggcaccgtt
     5761 tggtgtgcaa ctgggtacct agctgtagca agcactccac cgtctgtgca atcccccaat
     5821 ccacggcagg aacttcgcac cgccgcggca ccgagtgagc gaatccatcc gcattggatc
     5881 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     5941 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6001 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6361 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6601 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7921 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7981 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     8041 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     8101 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     8161 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     8221 gtatcataca catacgattt aggtgacact a
//
