LOCUS       40924_43198        6586 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora cloning vector pTH1124.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Hammond TM, Xiao H, Rehard DG, Boone EC, Perdue TD, Pukkila PJ, Shiu
            PK.
  TITLE     Fluorescent and bimolecular-fluorescent protein tagging of genes at 
            their native loci in Neurospora crassa using specialized 
            double-joint PCR plasmids
  JOURNAL   Fungal Genet. Biol. 48 (9), 866-873 (2011)
  PUBMED    21664475
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Hammond TM, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2011) Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 6586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6586)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2011"; volume: "48"; issue: "9"; pages: 
            "866-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2011) Biological Sciences, University of Missouri-Columbia, 
            103 Tucker Hall, Columbia, MO 65211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(337..360)
                     /label=4X(GlyAla)-linker
                     /note="4X(GlyAla)-linker"
     CDS             complement(361..612)
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     regulatory      complement(616..1538)
                     /label=ccg-1
                     /note="ccg-1"
                     /regulatory_class="promoter"
     CDS             complement(1585..2607)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(2612..2966)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(3020..3038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3045..3061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3202..3630
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3974..4765
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4786..5643
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5817..6405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttagca cccgcacccg ctcctgcccc
      361 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      421 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
      481 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      541 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      601 cttctgcttg tccattttgg ttgatgtgag gggttgtgaa cgtggtttga gtgttgtgtt
      661 gatttgaagt agtgaagatg tgattgcttt gttggctgat gatggaagaa gaaaagaatg
      721 gtattgggga ggtgggagga cagtcttata taggcatttg gttcccttta atcggcaatc
      781 gagatgacca agttggaagc ttgtgttcct ggctgcctgc actgcctgcc tggccctaag
      841 tggcatctac gatctcccgt cacagctcgc aaacaagcaa caacgggatg gaatcatgtg
      901 aagaagaagg agatacgtca taatcccaac gcggatggta tgttccggcc atctcgcctg
      961 gaggttcggg tggggcactc ggagaagtgg aggaggtgcc aattttggct tgcatgaaca
     1021 cttgcttctt ttttgacact cgacacactg caatcaagac gtaccttccg agctttgcat
     1081 tgtgggtatt caatagtggt ttcccagttg tttgcttgac atgtgcgcct tctttgcagg
     1141 caaattgcta gcaaagaagt gaggcaatct tcgtccttca cccgttgtct gtgacgtcac
     1201 gcccctttga acccctctcc cgcagttaga tgaaatctga atagcagcca ctgcacactc
     1261 aggatgacct ggtgcgacca ccaatgcttt gcccgaatcc atgtcacaat cgctgtctgt
     1321 gtgcttttct gttaccgaag gccgcgcgta gccgtgacat ggatataatg tggctgttga
     1381 aagcggccag aagtcggtct cgtccgatca atgcctgtcc caatggaagt gcccagtgat
     1441 gtggctttga acgaactgag tgacttctgg tagctgcatc catcgattca aagtagctga
     1501 ttctctcgtg attcacgcag atggactgct ccttctaaag ggcgaattct gcagataact
     1561 ggttcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt
     1621 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     1681 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     1741 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     1801 aatgcggagc atatacgccc ggaggcgcgg cgatcctgca agctccggat gcctccgctc
     1861 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga ggatgttggc
     1921 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     1981 gccattgtcc gtcaggacat tgttggagcc gaaatccgca tgcacgaggt gccggacttc
     2041 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     2101 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     2161 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     2221 ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct ggagaacagc
     2281 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
     2341 ataggtcagg ctctcgctga actccccaat gtcaagcact tccggaatcg ggagcgcggc
     2401 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     2461 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     2521 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     2581 cgtcgcggtg agttcaggct ttttcatttg gatgcttggg tagaataggt aagtcagatt
     2641 gaatctgaaa taaagggagg aagggcgaac ttaagaaggt atgaccgggt cgtccactta
     2701 ccttgcttga caaacgcacc aagttatcgt gcaccaagca gcagatgata ataatgtcct
     2761 cgttcctgtc tgctaataag agtcacactt cgagcgccgc cgctactgct acaagtgggg
     2821 ctgatctgac cagttgccta aatgaaccat cttgtcaaac gacacaaatt ttgtgctcac
     2881 cgcctggacg actaaaccaa aataggcatt cattgttgac ctccactagc tccagccaag
     2941 cccaaaaaat gctccttcaa tatcagttat ccatcacact ggcggccgct cgagcatgca
     3001 tctagagggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     3061 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3181 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3241 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3301 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3361 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3421 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3481 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3541 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3601 agctgattta acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta
     3661 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     3721 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     3781 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     3841 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     3901 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     3961 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4021 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4081 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4141 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4201 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4261 agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat
     4321 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4381 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4441 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4501 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4561 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4621 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4681 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4741 ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat
     4801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
