LOCUS       40924_43188        7048 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora cloning vector pTH1117.12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Hammond TM, Xiao H, Rehard DG, Boone EC, Perdue TD, Pukkila PJ, Shiu
            PK.
  TITLE     Fluorescent and bimolecular-fluorescent protein tagging of genes at 
            their native loci in Neurospora crassa using specialized 
            double-joint PCR plasmids
  JOURNAL   Fungal Genet. Biol. 48 (9), 866-873 (2011)
  PUBMED    21664475
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Hammond TM, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2011) Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 7048)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7048)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2011"; volume: "48"; issue: "9"; pages: 
            "866-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2011) Biological Sciences, University of Missouri-Columbia, 
            103 Tucker Hall, Columbia, MO 65211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(337..360)
                     /label=4X(GlyAla)-linker
                     /note="4X(GlyAla)-linker"
     CDS             complement(361..1077)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      complement(1078..2000)
                     /label=ccg-1
                     /note="ccg-1"
                     /regulatory_class="promoter"
     CDS             complement(2047..3069)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(3074..3428)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(3482..3500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3507..3523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3664..4092
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             4436..5227
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             5248..6105
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6279..6867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttagca cccgcacccg ctcctgcccc
      361 cttgtacagc tcgtccatgc cgtgagtgat cccggcggcg gtcacgaact ccagcaggac
      421 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      481 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      541 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      601 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      661 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      721 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      781 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      841 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtgaaggtgg tcacgagggt
      901 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      961 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     1021 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatttt
     1081 ggttgatgtg aggggttgtg aacgtggttt gagtgttgtg ttgatttgaa gtagtgaaga
     1141 tgtgattgct ttgttggctg atgatggaag aagaaaagaa tggtattggg gaggtgggag
     1201 gacagtctta tataggcatt tggttccctt taatcggcaa tcgagatgac caagttggaa
     1261 gcttgtgttc ctggctgcct gcactgcctg cctggcccta agtggcatct acgatctccc
     1321 gtcacagctc gcaaacaagc aacaacggga tggaatcatg tgaagaagaa ggagatacgt
     1381 cataatccca acgcggatgg tatgttccgg ccatctcgcc tggaggttcg ggtggggcac
     1441 tcggagaagt ggaggaggtg ccaattttgg cttgcatgaa cacttgcttc ttttttgaca
     1501 ctcgacacac tgcaatcaag acgtaccttc cgagctttgc attgtgggta ttcaatagtg
     1561 gtttcccagt tgtttgcttg acatgtgcgc cttctttgca ggcaaattgc tagcaaagaa
     1621 gtgaggcaat cttcgtcctt cacccgttgt ctgtgacgtc acgccccttt gaacccctct
     1681 cccgcagtta gatgaaatct gaatagcagc cactgcacac tcaggatgac ctggtgcgac
     1741 caccaatgct ttgcccgaat ccatgtcaca atcgctgtct gtgtgctttt ctgttaccga
     1801 aggccgcgcg tagccgtgac atggatataa tgtggctgtt gaaagcggcc agaagtcggt
     1861 ctcgtccgat caatgcctgt cccaatggaa gtgcccagtg atgtggcttt gaacgaactg
     1921 agtgacttct ggtagctgca tccatcgatt caaagtagct gattctctcg tgattcacgc
     1981 agatggactg ctccttctaa agggcgaatt ctgcagataa ctggttcccg gtcggcatct
     2041 actctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
     2101 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
     2161 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
     2221 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
     2281 ccggaggcgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
     2341 ctccatacaa gccaaccacg gcctccagaa gaggatgttg gcgacctcgt attgggaatc
     2401 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
     2461 attgttggag ccgaaatccg catgcacgag gtgccggact tcggggcagt cctcggccca
     2521 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
     2581 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
     2641 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
     2701 agcgatcgca tccatggcct ccgcgaccgg ctggagaaca gcgggcagtt cggtttcagg
     2761 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
     2821 gaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
     2881 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
     2941 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
     3001 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
     3061 ctttttcatt tggatgcttg ggtagaatag gtaagtcaga ttgaatctga aataaaggga
     3121 ggaagggcga acttaagaag gtatgaccgg gtcgtccact taccttgctt gacaaacgca
     3181 ccaagttatc gtgcaccaag cagcagatga taataatgtc ctcgttcctg tctgctaata
     3241 agagtcacac ttcgagcgcc gccgctactg ctacaagtgg ggctgatctg accagttgcc
     3301 taaatgaacc atcttgtcaa acgacacaaa ttttgtgctc accgcctgga cgactaaacc
     3361 aaaataggca ttcattgttg acctccacta gctccagcca agcccaaaaa atgctccttc
     3421 aatatcagtt atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg
     3481 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     3541 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3661 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3961 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4021 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4081 ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta
     4141 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     4201 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     4261 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     4321 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     4381 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     4441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4741 cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4981 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5221 gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5281 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5341 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5401 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5461 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5521 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5581 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5641 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5701 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5761 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5821 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5881 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5941 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6001 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6061 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6121 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6181 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6421 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7021 agcgagtcag tgagcgagga agcggaag
//
