LOCUS       40924_43183        6367 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora cloning vector pTH1112.8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Hammond TM, Xiao H, Rehard DG, Boone EC, Perdue TD, Pukkila PJ, Shiu
            PK.
  TITLE     Fluorescent and bimolecular-fluorescent protein tagging of genes at 
            their native loci in Neurospora crassa using specialized 
            double-joint PCR plasmids
  JOURNAL   Fungal Genet. Biol. 48 (9), 866-873 (2011)
  PUBMED    21664475
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Hammond TM, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2011) Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 6367)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2011"; volume: "48"; issue: "9"; pages: 
            "866-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2011) Biological Sciences, University of Missouri-Columbia, 
            103 Tucker Hall, Columbia, MO 65211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        348..702
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             707..1729
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      complement(1761..2229)
                     /label=pyrG
                     /note="pyrG"
                     /regulatory_class="terminator"
     CDS             complement(2242..2706)
                     /label=VN155(I152L)
                     /note="improved N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
     misc_feature    complement(2707..2730)
                     /label=4X(GlyAla)-linker
                     /note="4X(GlyAla)-linker"
     promoter        complement(2801..2819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2826..2842)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2983..3411
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3755..4546
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4567..5424
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5598..6186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttgggc ccgttaactg atattgaagg
      361 agcatttttt gggcttggct ggagctagtg gaggtcaaca atgaatgcct attttggttt
      421 agtcgtccag gcggtgagca caaaatttgt gtcgtttgac aagatggttc atttaggcaa
      481 ctggtcagat cagccccact tgtagcagta gcggcggcgc tcgaagtgtg actcttatta
      541 gcagacagga acgaggacat tattatcatc tgctgcttgg tgcacgataa cttggtgcgt
      601 ttgtcaagca aggtaagtgg acgacccggt cataccttct taagttcgcc cttcctccct
      661 ttatttcaga ttcaatctga cttacctatt ctacccaagc atccaaatga aaaagcctga
      721 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
      781 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
      841 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
      901 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agttcagcga
      961 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     1021 aaccgaactg cccgctgttc tccagccggt cgcggaggcc atggatgcga tcgctgcggc
     1081 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     1141 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     1201 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     1261 cgaggactgc cccgaagtcc ggcacctcgt gcatgcggat ttcggctcca acaatgtcct
     1321 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     1381 ccaatacgag gtcgccaaca tcctcttctg gaggccgtgg ttggcttgta tggagcagca
     1441 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcgcc tccgggcgta
     1501 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     1561 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     1621 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     1681 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     1741 ccgaccggga accagttaac gcttcgggta gagtatgtgg tcctgatagc aactgcccat
     1801 attttgctaa atgataaggt caataaaagc cggcttaacc acagcccaac tccaatcttc
     1861 aagacttttt tctagcccac tttctcaact ggaatcagtg gagcgaacca attctctccc
     1921 agccacaaag gaacctgact actacaatgt tggggatttc ctctgataga gacttttcag
     1981 tattggatgg tctgcgatca atcgtctagt tagggttaga gcacagccag agcctagatg
     2041 ctgcgttcca aggggtagat cagtgcttgt ctaccagatt agggagcata tcgttcagag
     2101 ctggtcacaa taatatctgt tcgaatacta ctagagccgt cagtgaggct ctttctggta
     2161 atactatgct ggctgcaata aacatatgga tcccttttag tcaataccgt tacacatttc
     2221 cactcacact cagggccctt aggccatgat atagacgttg tggctgttgt agttgtactc
     2281 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     2341 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     2401 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     2461 gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc cgaaggtggt
     2521 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     2581 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     2641 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     2701 caccatcgct ccagcacccg cacccgctcc aagggcgaat tctgcagata tccatcacac
     2761 tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac
     2821 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2881 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2941 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     3001 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3061 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3421 ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg
     3481 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     3541 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     3601 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     3661 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     3721 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3781 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3841 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3901 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     3961 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4021 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc
     4081 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4141 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4201 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4261 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4321 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4381 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4441 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4501 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg
     4561 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4681 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4741 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4801 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4861 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4921 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4981 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5101 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5161 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5221 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5461 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5521 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6241 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6301 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6361 gcggaag
//
