LOCUS       40924_43178        7308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora cloning vector pTH1111.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Hammond TM, Xiao H, Rehard DG, Boone EC, Perdue TD, Pukkila PJ, Shiu
            PK.
  TITLE     Fluorescent and bimolecular-fluorescent protein tagging of genes at 
            their native loci in Neurospora crassa using specialized 
            double-joint PCR plasmids
  JOURNAL   Fungal Genet. Biol. 48 (9), 866-873 (2011)
  PUBMED    21664475
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Hammond TM, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2011) Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 7308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2011"; volume: "48"; issue: "9"; pages: 
            "866-873"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2011) Biological Sciences, University of Missouri-Columbia, 
            103 Tucker Hall, Columbia, MO 65211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    337..360
                     /label=4X(GlyAla)-linker
                     /note="4X(GlyAla)-linker"
     CDS             361..1038
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     regulatory      1783..2251
                     /label=pyrG
                     /note="pyrG"
                     /regulatory_class="terminator"
     CDS             complement(2283..3305)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(3310..3664)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(3742..3760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3767..3783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3924..4352
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             4696..5487
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             5508..6365
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6539..7127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggag cgggtgcggg tgctggagcg
      361 atggtggcct cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
      421 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      481 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      541 atcctgtccc cccagttcca gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      601 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      661 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      721 tacaaggtga agttccgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      781 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      841 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      901 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga caccaagctg
      961 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     1021 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
     1081 gcctcctccg aggacgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     1141 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1201 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1261 tccccccagt tccagtacgg ctccaaggcg tacgtgaagc accccgccga catccccgac
     1321 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1381 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1441 gtgaagttcc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1501 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gagcgagatc
     1561 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1621 atggccaaga agcccgtgca gctgcccggc tactactacg tggacaccaa gctggacatc
     1681 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1741 cacctgttcc tgggtagtgg aggtggcgcg cctactagtt gagtgtgagt ggaaatgtgt
     1801 aacggtattg actaaaaggg atccatatgt ttattgcagc cagcatagta ttaccagaaa
     1861 gagcctcact gacggctcta gtagtattcg aacagatatt attgtgacca gctctgaacg
     1921 atatgctccc taatctggta gacaagcact gatctacccc ttggaacgca gcatctaggc
     1981 tctggctgtg ctctaaccct aactagacga ttgatcgcag accatccaat actgaaaagt
     2041 ctctatcaga ggaaatcccc aacattgtag tagtcaggtt cctttgtggc tgggagagaa
     2101 ttggttcgct ccactgattc cagttgagaa agtgggctag aaaaaagtct tgaagattgg
     2161 agttgggctg tggttaagcc ggcttttatt gaccttatca tttagcaaaa tatgggcagt
     2221 tgctatcagg accacatact ctacccgaag cgttaactgg ttcccggtcg gcatctactc
     2281 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
     2341 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
     2401 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
     2461 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
     2521 aggcgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
     2581 atacaagcca accacggcct ccagaagagg atgttggcga cctcgtattg ggaatccccg
     2641 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
     2701 ttggagccga aatccgcatg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
     2761 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
     2821 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
     2881 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
     2941 atcgcatcca tggcctccgc gaccggctgg agaacagcgg gcagttcggt ttcaggcagg
     3001 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaac
     3061 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
     3121 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
     3181 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
     3241 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
     3301 ttcatttgga tgcttgggta gaataggtaa gtcagattga atctgaaata aagggaggaa
     3361 gggcgaactt aagaaggtat gaccgggtcg tccacttacc ttgcttgaca aacgcaccaa
     3421 gttatcgtgc accaagcagc agatgataat aatgtcctcg ttcctgtctg ctaataagag
     3481 tcacacttcg agcgccgccg ctactgctac aagtggggct gatctgacca gttgcctaaa
     3541 tgaaccatct tgtcaaacga cacaaatttt gtgctcaccg cctggacgac taaaccaaaa
     3601 taggcattca ttgttgacct ccactagctc cagccaagcc caaaaaatgc tccttcaata
     3661 tcagttaact taagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
     3721 catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     3781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3901 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4321 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa attcagggcg caagggctgc
     4381 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     4441 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     4501 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     4561 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     4621 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     4681 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4741 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4801 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4861 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4921 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4981 gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc
     5041 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5101 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5161 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5221 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5281 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5341 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5401 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5461 ttctatcgcc ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac
     5521 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5581 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5641 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5701 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5761 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5821 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5881 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5941 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6001 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6061 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6121 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6181 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6241 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6301 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6361 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6421 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6661 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7201 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7261 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
