LOCUS       40924_43148        3417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTG8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Yang Y, Spector A.
  TITLE     Improved cloning vectors for transgene construction
  JOURNAL   BioTechniques 22 (6), 1032-1034 (1997)
  PUBMED    9187746
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Yang Y, Spector A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-1999) Institute of Molecular Biology, University 
            of Hong Kong, 8 Sassoon, Pokfulam, Hong Kong
REFERENCE   3  (bases 1 to 3417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1997"; volume: "22"; issue: "6"; pages: 
            "1032-1034"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-1999) Institute of Molecular Biology, University of Hong 
            Kong, 8 Sassoon, Pokfulam, Hong Kong"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        635..653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    662..769
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(782..800)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(830..846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(854..870)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(878..908)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(923..944)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1232..1820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2316..2867)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /protein_id="AAF61635.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPFSPWANIIRKATRC"
     promoter        complement(2868..2970)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(3290..3394)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcattaat taagtaatac gactcactat agggcgaatt
      661 ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt
      721 cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt
      781 ccctttagtg agggttaatt ggccggcctg cgcgcttggc gtaatcatgg tcatagctgt
      841 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      901 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     1681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1921 agatcctttt tgatcagaaa aaaaggatca tatcgtcaat tattacctcc acggggagag
     1981 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca
     2041 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     2101 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
     2161 ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     2221 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2281 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2341 atatttgccc atggtgaaaa cggggcgaag aagttgtcca tattggccac gtttaaatca
     2401 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2461 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2521 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2581 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2641 gccatacgta attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2701 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2761 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2821 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2881 gctcctgaaa atctcgacaa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2941 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag
     3001 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
     3061 acaggtattt attcggtcga aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3361 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
