LOCUS       40924_43094        5523 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-ble, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 5523)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5523)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2128
                     /label=BleoR
                     /note="antibiotic-binding protein"
     intron          2132..2649
                     /gene="IgapdhII"
     gene            2132..2649
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(2686..2704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2725..2741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2749..2765)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2773..2803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2818..2839)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3127..3715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3889..4746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4747..4851)
                     /label=AmpR promoter
     rep_origin      complement(4877..5332)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5474..5490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5497..5515
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggcagtag ctgcccctac ccacaacaac tactctagtc taggcaccct
       61 catttcatct atgcgtatcc gcattcagga agtgagcgaa gttcaacagt tgccagtttc
      121 ttcaggagac tcaaaaaaac agacacaaca gtcttattag acaagagcac aaacaaattc
      181 tcattgcatt cagaagcaaa caaacaaaca aaaacaaaat gcggaaaata cctcaaattt
      241 attttatgtc tgtcctctac aaataataat aataataata ataattacag cggccgtaaa
      301 tgaatgcaag aaaagaaaca caaaacccac aattgtcaac ctccccttca ttccacttgt
      361 ctctcttttc ccacgtttcc tgcacgaatg cagaaagtga tatttttact ttgaaagcca
      421 tctaccaaca acaattacat tgaacagaat tctagaacta gtggatcccc gggtaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaaagc ggccgcgact ctagaattcg atatcaagct taggcgtggc gatgacttca
     1261 ggtctttctt ttgcgaatag ggatcttata atacacgatg cgtgtcccgt gatgatcgtt
     1321 accggtgctg ccacgatcca actgacacag cgtcaagagc aaaacaattt tacttttccc
     1381 tttaaggaca acaacaaaaa aatatataac ttttttttct tttttttttt ttttgaaatt
     1441 atatttatgg tcatctttgg gaaacaaaaa gcagcaattt aatgatgcgg aaggatgagt
     1501 gaaataatgt ttaatcaatg tacgaggatt tggggtattg caaggaaaat gtagatgatt
     1561 taattgggtg tgtgatgcag cttgtggtaa tttttgctca cttccctttt tgccacatct
     1621 ttttagtttt tctgcttttc tttccccatt attccacttg tctctctttt cccacgtttc
     1681 ctgcacgaat gcagaaagtg atatttttac tttgaaagcc atctaccaac aacaattaca
     1741 ttgaacagaa tttaaaatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     1801 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     1861 gaacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     1921 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     1981 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     2041 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     2101 cgtgcacttc gtggccgagg agcaggacta ggcgtggcga tgacttcagg tctttctttt
     2161 gcgaataggg atcttataat acacgatgcg tgtcccgtga tggtcgttac cggtgctgcc
     2221 acgatccaag tgacacagtg tcaagagcaa aacaatttta cttttccctt taaggacaac
     2281 aacaaaaaaa tatataactt ttttttcttt tttttttttg aaattatatt tatggtcatc
     2341 tttgggaaac aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat
     2401 caatgtacga ggatttgggg tattgcaagg aaaatgtaga tgatttgatt gggtgtatga
     2461 tgcagcttgt ggtaattttt tacaactttg cgttattgga agattttggt tgttcagttt
     2521 ctttttgttt ataactgact gttttaattt agtgtgattt ttttttttaa ggatttaagc
     2581 tgtttttctt tttttttctt tttcttcact gatggtgttt aaggtgtagt ttagtgtttg
     2641 tgcattatgc atgcaagcta gccgtgtggg taccagcttt tgttcccttt agtgagggtt
     2701 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2761 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2821 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2881 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2941 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3001 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3061 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3541 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4861 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4921 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4981 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5041 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5101 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5161 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5221 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5281 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     5341 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     5401 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     5461 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     5521 ttg
//
