LOCUS       40924_43114        5748 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-PAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 5748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2353
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     intron          2357..2874
                     /gene="IgapdhII"
     gene            2357..2874
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(2911..2929)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2950..2966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2974..2990)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2998..3028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3043..3064)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3352..3940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4114..4971)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4972..5076)
                     /label=AmpR promoter
     rep_origin      complement(5102..5557)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5699..5715
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5722..5740
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggcagtag ctgcccctac ccacaacaac tactctagtc taggcaccct
       61 catttcatct atgcgtatcc gcattcagga agtgagcgaa gttcaacagt tgccagtttc
      121 ttcaggagac tcaaaaaaac agacacaaca gtcttattag acaagagcac aaacaaattc
      181 tcattgcatt cagaagcaaa caaacaaaca aaaacaaaat gcggaaaata cctcaaattt
      241 attttatgtc tgtcctctac aaataataat aataataata ataattacag cggccgtaaa
      301 tgaatgcaag aaaagaaaca caaaacccac aattgtcaac ctccccttca ttccacttgt
      361 ctctcttttc ccacgtttcc tgcacgaatg cagaaagtga tatttttact ttgaaagcca
      421 tctaccaaca acaattacat tgaacagaat tctagaacta gtggatcccc gggtaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaaagc ggccgcgact ctagaattcg atatcaagct taggcgtggc gatgacttca
     1261 ggtctttctt ttgcgaatag ggatcttata atacacgatg cgtgtcccgt gatgatcgtt
     1321 accggtgctg ccacgatcca actgacacag cgtcaagagc aaaacaattt tacttttccc
     1381 tttaaggaca acaacaaaaa aatatataac ttttttttct tttttttttt ttttgaaatt
     1441 atatttatgg tcatctttgg gaaacaaaaa gcagcaattt aatgatgcgg aaggatgagt
     1501 gaaataatgt ttaatcaatg tacgaggatt tggggtattg caaggaaaat gtagatgatt
     1561 taattgggtg tgtgatgcag cttgtggtaa tttttgctca cttccctttt tgccacatct
     1621 ttttagtttt tctgcttttc tttccccatt attccacttg tctctctttt cccacgtttc
     1681 ctgcacgaat gcagaaagtg atatttttac tttgaaagcc atctaccaac aacaattaca
     1741 ttgaacagaa tttaaaatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1801 cgtcccccgg gccgtacgca ccctcgcagc cgcgttcgcc gactaccccg ccacgcgcca
     1861 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggaggt
     1981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     2041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     2101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaacacca
     2161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     2221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     2281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     2341 caagcccggt gcctaggcgt ggcgatgact tcaggtcttt cttttgcgaa tagggatctt
     2401 ataatacacg atgcgtgtcc cgtgatggtc gttaccggtg ctgccacgat ccaagtgaca
     2461 cagtgtcaag agcaaaacaa ttttactttt ccctttaagg acaacaacaa aaaaatatat
     2521 aacttttttt tctttttttt ttttgaaatt atatttatgg tcatctttgg gaaacaaaaa
     2581 gcagcaattt aatgatgcgg aaggatgagt gaaataatgt ttaatcaatg tacgaggatt
     2641 tggggtattg caaggaaaat gtagatgatt tgattgggtg tatgatgcag cttgtggtaa
     2701 ttttttacaa ctttgcgtta ttggaagatt ttggttgttc agtttctttt tgtttataac
     2761 tgactgtttt aatttagtgt gatttttttt tttaaggatt taagctgttt ttcttttttt
     2821 ttctttttct tcactgatgg tgtttaaggt gtagtttagt gtttgtgcat tatgcatgca
     2881 agctagccgt gtgggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc
     2941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3781 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5101 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5161 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5221 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5281 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5341 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5401 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5461 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5521 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     5581 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5641 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5701 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattg
//
