LOCUS       40924_43109        5943 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-Neo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 5943)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5943)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2548
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     intron          2552..3069
                     /gene="IgapdhII"
     gene            2552..3069
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3106..3124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3145..3161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3169..3185)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3193..3223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3238..3259)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3547..4135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4309..5166)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5167..5271)
                     /label=AmpR promoter
     rep_origin      complement(5297..5752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5894..5910
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5917..5935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggcagtag ctgcccctac ccacaacaac tactctagtc taggcaccct
       61 catttcatct atgcgtatcc gcattcagga agtgagcgaa gttcaacagt tgccagtttc
      121 ttcaggagac tcaaaaaaac agacacaaca gtcttattag acaagagcac aaacaaattc
      181 tcattgcatt cagaagcaaa caaacaaaca aaaacaaaat gcggaaaata cctcaaattt
      241 attttatgtc tgtcctctac aaataataat aataataata ataattacag cggccgtaaa
      301 tgaatgcaag aaaagaaaca caaaacccac aattgtcaac ctccccttca ttccacttgt
      361 ctctcttttc ccacgtttcc tgcacgaatg cagaaagtga tatttttact ttgaaagcca
      421 tctaccaaca acaattacat tgaacagaat tctagaacta gtggatcccc gggtaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaaagc ggccgcgact ctagaattcg atatcaagct taggcgtggc gatgacttca
     1261 ggtctttctt ttgcgaatag ggatcttata atacacgatg cgtgtcccgt gatgatcgtt
     1321 accggtgctg ccacgatcca actgacacag cgtcaagagc aaaacaattt tacttttccc
     1381 tttaaggaca acaacaaaaa aatatataac ttttttttct tttttttttt ttttgaaatt
     1441 atatttatgg tcatctttgg gaaacaaaaa gcagcaattt aatgatgcgg aaggatgagt
     1501 gaaataatgt ttaatcaatg tacgaggatt tggggtattg caaggaaaat gtagatgatt
     1561 taattgggtg tgtgatgcag cttgtggtaa tttttgctca cttccctttt tgccacatct
     1621 ttttagtttt tctgcttttc tttccccatt attccacttg tctctctttt cccacgtttc
     1681 ctgcacgaat gcagaaagtg atatttttac tttgaaagcc atctaccaac aacaattaca
     1741 ttgaacagaa tttaaaatgc ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     1801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     1861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     1921 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     1981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     2281 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     2341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     2401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     2461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     2521 cttctatcgc cttcttgacg agttcttcta ggcgtggcga tgacttcagg tctttctttt
     2581 gcgaataggg atcttataat acacgatgcg tgtcccgtga tggtcgttac cggtgctgcc
     2641 acgatccaag tgacacagtg tcaagagcaa aacaatttta cttttccctt taaggacaac
     2701 aacaaaaaaa tatataactt ttttttcttt tttttttttg aaattatatt tatggtcatc
     2761 tttgggaaac aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat
     2821 caatgtacga ggatttgggg tattgcaagg aaaatgtaga tgatttgatt gggtgtatga
     2881 tgcagcttgt ggtaattttt tacaactttg cgttattgga agattttggt tgttcagttt
     2941 ctttttgttt ataactgact gttttaattt agtgtgattt ttttttttaa ggatttaagc
     3001 tgtttttctt tttttttctt tttcttcact gatggtgttt aaggtgtagt ttagtgtttg
     3061 tgcattatgc atgcaagcta gccgtgtggg taccagcttt tgttcccttt agtgagggtt
     3121 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5281 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5341 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5401 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5461 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5521 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5581 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5641 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5701 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     5761 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     5821 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     5881 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     5941 ttg
//
