LOCUS       40924_43104        6174 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2779
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          2783..3300
                     /gene="IgapdhII"
     gene            2783..3300
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3337..3355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3400..3416)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3424..3454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3469..3490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3778..4366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4540..5397)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5398..5502)
                     /label=AmpR promoter
     rep_origin      complement(5528..5983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6125..6141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6148..6166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggcagtag ctgcccctac ccacaacaac tactctagtc taggcaccct
       61 catttcatct atgcgtatcc gcattcagga agtgagcgaa gttcaacagt tgccagtttc
      121 ttcaggagac tcaaaaaaac agacacaaca gtcttattag acaagagcac aaacaaattc
      181 tcattgcatt cagaagcaaa caaacaaaca aaaacaaaat gcggaaaata cctcaaattt
      241 attttatgtc tgtcctctac aaataataat aataataata ataattacag cggccgtaaa
      301 tgaatgcaag aaaagaaaca caaaacccac aattgtcaac ctccccttca ttccacttgt
      361 ctctcttttc ccacgtttcc tgcacgaatg cagaaagtga tatttttact ttgaaagcca
      421 tctaccaaca acaattacat tgaacagaat tctagaacta gtggatcccc gggtaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaaagc ggccgcgact ctagaattcg atatcaagct taggcgtggc gatgacttca
     1261 ggtctttctt ttgcgaatag ggatcttata atacacgatg cgtgtcccgt gatgatcgtt
     1321 accggtgctg ccacgatcca actgacacag cgtcaagagc aaaacaattt tacttttccc
     1381 tttaaggaca acaacaaaaa aatatataac ttttttttct tttttttttt ttttgaaatt
     1441 atatttatgg tcatctttgg gaaacaaaaa gcagcaattt aatgatgcgg aaggatgagt
     1501 gaaataatgt ttaatcaatg tacgaggatt tggggtattg caaggaaaat gtagatgatt
     1561 taattgggtg tgtgatgcag cttgtggtaa tttttgctca cttccctttt tgccacatct
     1621 ttttagtttt tctgcttttc tttccccatt attccacttg tctctctttt cccacgtttc
     1681 ctgcacgaat gcagaaagtg atatttttac tttgaaagcc atctaccaac aacaattaca
     1741 ttgaacagaa tttaaaatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     1801 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     1861 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     1921 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     1981 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     2041 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     2101 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     2161 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     2221 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     2281 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     2341 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     2401 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     2461 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     2521 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     2581 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     2641 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     2701 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     2761 tcgtccgagg gcaaaggact aggcgtggcg atgacttcag gtctttcttt tgcgaatagg
     2821 gatcttataa tacacgatgc gtgtcccgtg atggtcgtta ccggtgctgc cacgatccaa
     2881 gtgacacagt gtcaagagca aaacaatttt acttttccct ttaaggacaa caacaaaaaa
     2941 atatataact tttttttctt tttttttttt gaaattatat ttatggtcat ctttgggaaa
     3001 caaaaagcag caatttaatg atgcggaagg atgagtgaaa taatgtttaa tcaatgtacg
     3061 aggatttggg gtattgcaag gaaaatgtag atgatttgat tgggtgtatg atgcagcttg
     3121 tggtaatttt ttacaacttt gcgttattgg aagattttgg ttgttcagtt tctttttgtt
     3181 tataactgac tgttttaatt tagtgtgatt ttttttttta aggatttaag ctgtttttct
     3241 ttttttttct ttttcttcac tgatggtgtt taaggtgtag tttagtgttt gtgcattatg
     3301 catgcaagct agccgtgtgg gtaccagctt ttgttccctt tagtgagggt taatttcgag
     3361 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3421 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3481 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3541 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3601 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3661 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3721 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3781 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3841 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3901 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3961 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4021 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4081 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4141 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4201 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4261 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4321 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4381 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4441 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4501 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4561 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4621 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4681 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4741 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4801 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4861 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4921 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4981 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5041 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5101 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5161 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5221 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5281 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5341 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5401 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5461 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5521 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     5581 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     5641 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5701 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5761 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5821 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5881 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5941 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     6001 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6061 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6121 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attg
//
