LOCUS       40924_43099        5547 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-BSD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 5547)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5547)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2152
                     /label=BSD
                     /note="blasticidin S deaminase"
     intron          2156..2673
                     /gene="IgapdhII"
     gene            2156..2673
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(2710..2728)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2749..2765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2773..2789)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2797..2827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2842..2863)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3151..3739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3913..4770)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4771..4875)
                     /label=AmpR promoter
     rep_origin      complement(4901..5356)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5498..5514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5521..5539
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggcagtag ctgcccctac ccacaacaac tactctagtc taggcaccct
       61 catttcatct atgcgtatcc gcattcagga agtgagcgaa gttcaacagt tgccagtttc
      121 ttcaggagac tcaaaaaaac agacacaaca gtcttattag acaagagcac aaacaaattc
      181 tcattgcatt cagaagcaaa caaacaaaca aaaacaaaat gcggaaaata cctcaaattt
      241 attttatgtc tgtcctctac aaataataat aataataata ataattacag cggccgtaaa
      301 tgaatgcaag aaaagaaaca caaaacccac aattgtcaac ctccccttca ttccacttgt
      361 ctctcttttc ccacgtttcc tgcacgaatg cagaaagtga tatttttact ttgaaagcca
      421 tctaccaaca acaattacat tgaacagaat tctagaacta gtggatcccc gggtaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaaagc ggccgcgact ctagaattcg atatcaagct taggcgtggc gatgacttca
     1261 ggtctttctt ttgcgaatag ggatcttata atacacgatg cgtgtcccgt gatgatcgtt
     1321 accggtgctg ccacgatcca actgacacag cgtcaagagc aaaacaattt tacttttccc
     1381 tttaaggaca acaacaaaaa aatatataac ttttttttct tttttttttt ttttgaaatt
     1441 atatttatgg tcatctttgg gaaacaaaaa gcagcaattt aatgatgcgg aaggatgagt
     1501 gaaataatgt ttaatcaatg tacgaggatt tggggtattg caaggaaaat gtagatgatt
     1561 taattgggtg tgtgatgcag cttgtggtaa tttttgctca cttccctttt tgccacatct
     1621 ttttagtttt tctgcttttc tttccccatt attccacttg tctctctttt cccacgtttc
     1681 ctgcacgaat gcagaaagtg atatttttac tttgaaagcc atctaccaac aacaattaca
     1741 ttgaacagaa tttaaaatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     1801 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     1861 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     1921 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     1981 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     2041 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     2101 gacggcagtt gggattcgtg aactgctgcc ctctggttat gtgtgggagg gctaggcgtg
     2161 gcgatgactt caggtctttc ttttgcgaat agggatctta taatacacga tgcgtgtccc
     2221 gtgatggtcg ttaccggtgc tgccacgatc caagtgacac agtgtcaaga gcaaaacaat
     2281 tttacttttc cctttaagga caacaacaaa aaaatatata actttttttt cttttttttt
     2341 tttgaaatta tatttatggt catctttggg aaacaaaaag cagcaattta atgatgcgga
     2401 aggatgagtg aaataatgtt taatcaatgt acgaggattt ggggtattgc aaggaaaatg
     2461 tagatgattt gattgggtgt atgatgcagc ttgtggtaat tttttacaac tttgcgttat
     2521 tggaagattt tggttgttca gtttcttttt gtttataact gactgtttta atttagtgtg
     2581 attttttttt ttaaggattt aagctgtttt tctttttttt tctttttctt cactgatggt
     2641 gtttaaggtg tagtttagtg tttgtgcatt atgcatgcaa gctagccgtg tgggtaccag
     2701 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     2761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2821 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2881 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2941 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3001 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3061 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5341 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5401 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5461 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     5521 taatacgact cactataggg cgaattg
//
