LOCUS       V002748                 5874 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002748
VERSION     V002748
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Meiers M, Laux A, Eichinger D, Sexauer A, Marx P, Bertram R,
            Bruckner R.
  TITLE     A tetracycline inducible integrative expression system for
            Streptococcus pneumoniae
  JOURNAL   FEMS Microbiol. Lett. (2017) In press
   PUBMED   28333198
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Bruckner R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2016) Microbiology, University of Kaiserslautern,
            Paul-Ehrlich Str. 24, Kaiserslautern, RP D-67663, Germany
REFERENCE   3  (bases 1 to 5874)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5874)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett. (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2016) Microbiology, University of Kaiserslautern,
            Paul-Ehrlich Str. 24, Kaiserslautern, RP D-67663, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..70
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    complement(87..105)
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             212..835
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     misc_recomb     910..1894
                     /label="integration site S. pneumoniae"
                     /note="integration site S. pneumoniae"
     misc_feature    2036..2176
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2362..2950)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3124..3981)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3982..4086)
                     /label="AmpR promoter"
     misc_recomb     4191..5252
                     /label="integration site S. pneumoniae"
                     /note="integration site S. pneumoniae"
     CDS             complement(5303..5797)
                     /gene="dfrA13"
                     /label="Dihydrofolate reductase type A13"
                     /note="Dihydrofolate reductase type A13 from Escherichia
                     coli. Accession#: Q59408"
ORIGIN
        1 ctagcgcatg gtaccttgac ccgggagcct ctagagcaag gatctgcatc atactctatc
       61 aatgatagag agcttatttt aattatactc tatcaatgat agagtgtcaa tatttttttt
      121 agtttttcat gaactcgagg ggatccaaat aaaaaactag tttgacaaat aactctatca
      181 atgatataat gtcaacaaaa aggaggaatt aatgatgtca agattagata aaagtaaagt
      241 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
      301 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
      361 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
      421 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
      481 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
      541 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
      601 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
      661 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
      721 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
      781 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaaaa
      841 gcagcataac ctttttccgt gatggtaact tcacggtaac caagatgtcg agttatcgat
      901 accgtcgaca atctgtctac cttcaggaga taaaagcgcc tgacattctt tagctttcaa
      961 gttttgatag cgttcgttca tatacagtcc cttttgaggg actgattcag gttcatcagc
     1021 gtagtaaacc ttgatttcct gttgaaagtc tgtctgtgtt ttctgatgtt tggtatggtg
     1081 aaaacgataa taccagccat caggatgtgt gtagctatcc tccttgtcat tatagtgcca
     1141 attcgctaag tttttagctg actgtttatg tcctctcttt tgtttcttat caaacatggc
     1201 atatttaatc agaaggggag gatttcttct tgaaaataga cctgtatctg ttcttgaagt
     1261 ttggcggaga atttctctgt cgttttcttc cacacaaaac tatacttgtt ggcattggct
     1321 tcaatcttag ttccgtcaat aaacaaacaa tctaaggtca ctaactcttc catttttcaa
     1381 tttttcgacc agagaaaatc ccttgtgaat aggcaaatag aagagtagat acaagcattt
     1441 taaggtgata agacgggcga tcaaaggcat gatagaaggc gtagaagtga tgttcctcca
     1501 aggtattcac cactttttca atagtaaaga cgagatggtc ttgtggtaag aaggaactga
     1561 tttctagtgg taaagttgtt tgatttgtgt tatagtgaat atgcatggga tagcctttct
     1621 aaatggttta ttgggcaatt ctattttacc aagactatcg taaccatgca aaaaaagcaa
     1681 cggcaaatcc gttgcttttt ttggagataa gagactattg tcccagactc ttttctttgt
     1741 ataaaattgt atagtgttat tgtctatact attttgtaga gaaagcaaaa atccctagaa
     1801 aatgcttagg gattcgacta tttcattaag aacaattcaa caatcatttc tatatacatg
     1861 ataagggtta ataagaatcc tgcacgccag aagactgcct cgcgcgtttc ggtgatgacg
     1921 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     1981 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     2041 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     2101 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     2161 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2761 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3061 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3121 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3181 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3241 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3301 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3361 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3421 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3481 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3541 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3601 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3661 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3721 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     3781 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3841 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3901 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3961 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4021 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4081 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4141 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tccagaagta
     4201 tcagcgggtg tgatcttccg tgaaaatggt aagattgtag aacggactgt tgctcaagcc
     4261 aaacttcact tctttgcaga tagtggtacg gatgcaccat ctaaactcgt tttagaacgc
     4321 tatgtcggcc caggctttga agtacctacc tactattcaa actaccaagc ctacgaatct
     4381 ggacatccat ttaacaatcc agaaaattgg gaagctgtgc cttatcgtgc ggataaagac
     4441 atcgcagctg gtgatgaaat caacgtaaca tttaaagctg tcaaagccaa agtcatgaga
     4501 tggcgtatgg agcgtaaagc tgacaagagc ggtgttgcga tgattgagat gaccttcctt
     4561 gcaccaagtg aattgcctca agaaagcact caatcaaaga ttcttgtaga tggaaaagaa
     4621 cttgctgatt tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg tcaacggcca
     4681 aaagtctcag ttgaagaaaa caatcaagta gcttcaactg tggtagatag tggagaagat
     4741 agccttccag tacttgttcg cctcgtttca gaaagtggaa aacaagtcaa ggaataccgt
     4801 atccagttga ctaaggaaaa accagtttct gctgtacaag aagatcttcc aaaactcgaa
     4861 tttgttgaaa aagatttggc ctacaagaca gttgagaaaa aagattcaac actgtatcta
     4921 ggtgaaactc gtgtagaaca agaaggaaaa gttggaaaag aacgtatctt tacagtgatt
     4981 aatcctgatg gaagtaagga agaaaaactc cgtgaagtgg tagaagttcc gacagaccgc
     5041 atcgtcttgg ttggaaccaa accagtagct caagaagcta aaaaaccaca agtgtcagaa
     5101 aaagcagata caaaaccaat tgattcaagt gaagctgatc aaactaataa agcccagtta
     5161 ccaaatacag gtagtgcggc aagccaagca gcagtagcag caggtttagc tcttctaggt
     5221 ttgagtgcag gattagtagt tactaaaggt aagcttaaat aaaacggctg tcctgcaaat
     5281 gaggacagcc gtttttgcgt taaccgttac gacgcgcata gacggagtgc gtgtacgtga
     5341 ttgtgccttg aatggtttcg gatgagacaa cctcgaattc tgctgcgtta agcactggga
     5401 agaaggcgtc accctcaaag gtttgatgta cctcagatag aaagacgccg ttggcatgcg
     5461 gtagcgccag cgcatatacc tcggctccgc gcgctacgta gagttctttg ccgtgttcgg
     5521 ctgcgatggc gatagcctgt gacagcgttg aaacaactgc acaaccagga gcgctataac
     5581 cagcttggcg cgagagcacc actgtgtggc ggtttggtaa gggcttgcct atggactcaa
     5641 atgtcttgcg gcccataacg accactttgc tctcggtgag cctgcgaaaa atcttctgct
     5701 cacctgggat tttccagggg atatcgggac cattgccaat aacccgattg gcacccatgg
     5761 cagcgaccag ataaatgcgg accgattccg ggttcatatg aaaattcctc cgaatatttt
     5821 ttacctacct agtatagcat tttgtgaagt ttttttctag tccaagctca caag
//
