LOCUS       40924_43074        6625 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEX-Z, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Lin SL.
  TITLE     Salmonella-mediated gene transfer into macrophages
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Lin SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-1999) Psychiatry, Robert Wood Johnson Medical 
            School, 675 Hoes Lane, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 6625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-1999) Psychiatry, Robert Wood Johnson Medical School, 675 
            Hoes Lane, Piscataway, NJ 08854, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..374
                     /label=polyoma origin of replication
                     /note="polyoma origin of replication"
     enhancer        402..781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        782..985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1030..1048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1109..1126
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             1130..1162
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             1166..1189
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     CDS             1220..4264
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     intron          4392..4621
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     polyA_signal    4680..4903
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4989..5577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5751..6608)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tatacgcctc caaggcgcca gtcctgtttt gacaagttgc ctctggaagc ctctacaatg
       61 cctctcttct ttttctccag agtaagcggg ccaggggtcc ccggcctctg cttaatacta
      121 aaaaaaacag ctgttgtcat agtaatgatt gggtggaaac attctaggcc tgggtggaga
      181 ggctttttgc ttcctcttgc aaaaccacac tgccctctgg agggcagttg cctagcaact
      241 aattaaaaga ggatgtcgca cggccagctg cggtcagtta gtcacttcct gcttaactga
      301 cttgacattt tctattttaa gagtcgggag gaaaattact gtgttggagg ccctccgcca
      361 tcttctgaag ctgatcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      421 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      481 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      541 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      601 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      661 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      721 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      781 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      841 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      901 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      961 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     1021 tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatcga
     1081 taaacttaag cttaccatgg ggggttctca tcatcatcat catcatggta tggctagcat
     1141 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg tacctaagga
     1201 tcagcttgga gttgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1261 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1321 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
     1381 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
     1441 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
     1501 cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
     1561 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
     1621 ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg
     1681 ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa
     1741 ccgcctcgcg gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat
     1801 gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat
     1861 cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc
     1921 tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca
     1981 gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg
     2041 tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag
     2101 cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct
     2161 gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct
     2221 gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct
     2281 gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa
     2341 caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg
     2401 cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc
     2461 aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg
     2521 aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc
     2581 aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg
     2641 cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat
     2701 gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa
     2761 atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat
     2821 gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt
     2881 acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg
     2941 caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg
     3001 tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca
     3061 ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata
     3121 cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc
     3181 gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact
     3241 gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca
     3301 accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct
     3361 ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac
     3421 cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc
     3481 aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga
     3541 tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga
     3601 ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt
     3661 gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg
     3721 gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg
     3781 tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat
     3841 tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc
     3901 gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt
     3961 gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg
     4021 cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta
     4081 cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac
     4141 atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc
     4201 agtatcggcg gagttccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
     4261 aaaataataa agccgaattc tgcagatatc cagcacagtg gcggccgcgt cgacggaatt
     4321 cagtggatcc actagtaacg gccgccagtg tgctggaatt aattcgctgt ctgcgagggc
     4381 cggctgttgg ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc
     4441 agtttccaaa aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt
     4501 ggccgcgtcc atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag
     4561 gcttgagatc tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca
     4621 ggtgtccact cccaggtcca actgcaggtc gatcgagcaa gctcgctgat cagcctcgac
     4681 tgtgcctcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4741 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4801 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4861 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4921 accagctggg gctgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5161 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     5221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     5461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5821 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5881 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5941 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6001 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6061 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6361 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6601 atactcatac tcttcctttt tcaat
//
