LOCUS       V002758                 6151 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002758
VERSION     V002758
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Huber J, Donald RG, Lee SH, Jarantow LW, Salvatore MJ, Meng X,
            Painter R, Onishi RH, Occi J, Dorso K, Young K, Park YW, Skwish S,
            Szymonifka MJ, Waddell TS, Miesel L, Phillips JW, Roemer T.
  TITLE     Chemical genetic identification of peptidoglycan inhibitors
            potentiating carbapenem activity against methicillin-resistant
            Staphylococcus aureus
  JOURNAL   Chem. Biol. 16 (8), 837-848 (2009)
   PUBMED   19716474
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Trawick J, Haselbeck B.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem.
            Biol."; date: "2009"; volume: "16"; issue: "8"; pages: "837-848"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAR-2009) Infectious Diseases, Merck "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    210..231
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        246..276
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    284..300
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..324
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(352..975)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    997..1015
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     regulatory      1046..1107
                     /label="hybrid xylose/tetracycline promoter"
                     /note="hybrid xylose/tetracycline promoter"
                     /regulatory_class="promoter"
     protein_bind    1072..1089
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     terminator      1324..1410
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1502..1529
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(1675..2532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2533..2637)
                     /label="AmpR promoter"
     rep_origin      2857..3445
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4245..4892)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      5049..5212
                     /note="for replication in Staphylococcus sp."
ORIGIN
        1 caaccgtaat atatctatca aattttttat atttggaaac aaaaagagta tttagctagt
       61 gaactttatg acttaacaga acaagaaaaa agagaaatta atcacaaaat gatagacgaa
      121 atcgaggaag aacaataaca aaatataagt gctaacagct ggcacgacag gtttcccgac
      181 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      241 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      301 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgctt aagacccact
      361 ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc
      421 tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact
      481 atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac
      541 gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa
      601 accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg
      661 ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact
      721 tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt
      781 atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac
      841 atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa
      901 accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc
      961 taatctagac atcattaatt cctccttttt gttgacactc tatcattgat agagttattt
     1021 gtcaaactag ttttttattt ggtaccttct tcataaaaaa tttatttgct tctctatcat
     1081 tgatagagta taatagattt ccagaaagga tcccgggaat tcgtcgacct gcagccaagc
     1141 ttgggctttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     1201 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     1261 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     1321 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1381 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     1441 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     1501 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt
     1561 tctaaataca ttcaaatatg tatccgctga tccccatcct atcgatgata agctgtcaaa
     1621 catgagaatt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1681 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1741 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1801 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1861 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1921 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1981 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2041 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2101 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2161 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2221 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2281 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2341 aaaacgctct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2401 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2461 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2521 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2581 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2641 atttccccga aaagtgccac ctggcggccg cctaagaaac cattattatc atgacattaa
     2701 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaaaa ggatctaggt
     2761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3001 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3241 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3481 cttttgctgg ccttttgctc agcggccgct tctttcctgc gttatcccct gattctgtgg
     3541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3601 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3661 atctgtgcgg tatttcacac cgcataagat cccctcgacc tgcaggcatg caagcttctg
     3721 taggttttta ggcataaaac tatatgattt acccctaaat ctttaaaatg ccccttaaaa
     3781 ttcaaaataa aggcatttaa aatttaaata tttcttgtga taaagtttgt taaaaaggag
     3841 tggttttatg actgttatgt ggttatcgat tataggtatg tggttttgta ttggaatggc
     3901 attttttgct atcaaggtta ttaaaaataa aaattagacc acgcatttat gccgagaaaa
     3961 tttattgtgc gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
     4021 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt
     4081 caataaagca attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat
     4141 atggatctgg agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt
     4201 agaaaaccga ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta
     4261 ggcctatctg acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa
     4321 acagaatgat gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat
     4381 tttcctgctg taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac
     4441 ccagtaaatg aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt
     4501 ttgggaaaca atttccccga accattatat ttctctacat cagaaaggta taaatcataa
     4561 aactctttga agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca
     4621 tcaaaaattg tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg
     4681 taaccagttc taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca
     4741 gggtaaaatt tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct
     4801 gtggttatac taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc
     4861 caattgtcta aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa
     4921 agtgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag
     4981 tcaatattac tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt
     5041 ttgttgaact aatgggtgct ttagttgaag aataaagacc acattaaaaa atgtggtctt
     5101 ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt atcttgataa
     5161 taagggtaac tattgccggc gaggctagtt acccttaagt tattggtatg actggtttta
     5221 agcgcaaaaa aagttgcttt ttcgtaccta ttaatgtatc gttttaaatg actagtaaaa
     5281 aacatacata gaaaggggaa aaagcaactt tttttattgt catagtttgt gaaaactaag
     5341 ttgtttttat gtgttataac atggaaaagt atactgagaa aaaacaaaga aatcaagtat
     5401 ttcagaaatt tattaaacgt catattggag agaatcaaat ggatttagtt gaagattgca
     5461 atacatttct gtcttttgta gctgataaaa ctttagaaaa acagaaatta tataaagcta
     5521 attcttgtaa aaatcgattt tgtcctgtct gtgcttggag aaaagctaga aaagatgcat
     5581 tgggtttatc tttgatgatg caatatatta agcagcaaga gaaaaaggag tttatctttt
     5641 taactttgac tacacctaat gtaatgagtg atgaattaga aaatgaaata aaacgttata
     5701 ataattcttt tagaaaactt ataaagagaa aaaaagtagg tagtgttata aagggatatg
     5761 ttcgtaagtt agagattaca tataataaaa aaagagatga ttataatcct cattttcatg
     5821 tgttaattgc agtaaataaa tcgtatttca cagataaaag atattatatt agccaacaag
     5881 aatggttaga tttatggcgt gatgtaacgg gcatttcaga aataacacaa gttcaagttc
     5941 aaaaaataag acaaaataat aataaagaat tatatgaaat ggctaagtat tctggtaaag
     6001 atagtgatta tttaataaat aaatcaaaaa gtctttgatg cattttataa atcacttaaa
     6061 ggtaaacagg tattagttta ttcaggatta tttaaagagg ctaaaaagaa attaaaaaat
     6121 ggggatttag attacttaaa agaaattgat c
//
