LOCUS       pTEF1_Zeo.        3556 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid encoding a yeast expression cassette for a Zeocin(TM) 
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pTEF1 Zeo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid lacks sequences for selection and replication in yeast.
FEATURES             Location/Qualifiers
     source          1..3556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1875..1937
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     promoter        1937..2344
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2352..2399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2418..2789
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2858..3105
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3111..3156
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     primer_bind     complement(3160..3176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg tgtgctgggc ccagccggcc agatctgagc tcgcggccgc
     1921 gatatcgcta gctcgagccc acacaccata gcttcaaaat gtttctactc cttttttact
     1981 cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc
     2041 atactaaatt ttccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt
     2101 ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt
     2161 tttatcacgt ttctttttct tgaaattttt ttttttagtt tttttctctt tcagtgacct
     2221 ccattgatat ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct
     2281 tgttctatta caactttttt tacttcttgt tcattagaaa gaaagcatag caatctaatc
     2341 taaggggcgg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     2401 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     2461 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     2521 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     2581 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     2641 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     2701 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     2761 gcgtgcactt cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca
     2821 ggcctcggag atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc
     2881 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     2941 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     3001 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     3061 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct gaattcccgg
     3121 ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg
     3181 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3241 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3301 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3361 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3421 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3481 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3541 tcaccgaaac gcgcga
//
