LOCUS       40924_42949        5511 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phage display vector pTDisp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Kolesnikov AV, Kozyr AV, Zakharova MY.
  TITLE     Improved purification of filamentous bacteriophages for phage 
            display using size-exclusion chromatography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Kolesnikov AV, Kozyr AV, Zakharova MY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and 
            Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 
            16/10, Moscow 117997, Russia
REFERENCE   3  (bases 1 to 5511)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5511)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and Ovchinnikov 
            Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow 
            117997, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(145..1224)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1225..1302)
                     /label=lacI promoter
     protein_bind    1472..1488
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1539..1561
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1683..2231
                     /gene="pIII"
                     /note="m13 minor coat protein fragment; this fragment of
                     the protein is used for N-terminal fusion expression during
                     phage display"
     gene            1683..2231
                     /gene="pIII"
                     /label=pIII
     RBS             2251..2273
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     2281..2346
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     terminator      2566..2652
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2744..2771
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      2869..3324
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3606..3710
                     /label=AmpR promoter
     CDS             3711..4568
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4742..5330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5333..5339
                     /label=ColE1 ori
                     /note="ColE1 ori"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgga
       61 ctgggttgaa ggctctcaag ggcagtcggt cgagatcccg gtgcctaatg agtgagctaa
      121 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
      241 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
      301 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
      361 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
      421 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
      481 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
      541 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
      601 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
      661 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
      721 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
      781 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
      841 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
      901 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
      961 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     1021 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     1081 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     1141 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     1201 atcgtataac gttactggtt tcacattcac catcctgaat tgactctctt ccgggcgcta
     1261 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     1321 ccttatgcga ctcctgcatt aggccatgga ctccccatcc ccctgttgac aattaatcat
     1381 cggctcgtat aaaatacgct gaggacgtaa tccggtacct gaggggtagg gggacaactg
     1441 ttaattagta gccgagcata tatgtgtgga attgtgagcg gataacaatt tcacacagga
     1501 aacagaattg atccataact aactaatcta gaaataattt tgtttaactt taagaaggag
     1561 atatacatat gaaatacctg tccctgccga ccgctgctgc tggtctgctg ctcctcgcgg
     1621 cccagccggc catggcccag gtccaactgc aggagctcga ggaatttaaa tctgcggccg
     1681 caggtggtgg tccggttaat tctggcggct ctgagggtgg cggttctgag ggtggcggct
     1741 ctgagggtgg cggttccggt ggtggctctg gttccggtga ttttgattat gaaaagatgg
     1801 caaacgctaa taagggggct atgaccgaaa atgccgatga aaacgcgcta cagtctgacg
     1861 ctaaaggcaa acttgattct gtcgctactg attacggtgc tgctatcgac ggtttcattg
     1921 gtgacgtttc cggccttgct aatggtaatg gtgctactgg tgattttgct ggctctaatt
     1981 cccaaatggc tcaagtcggt gacggtgata attcaccttt aatgaataat ttccgtcaat
     2041 atttaccttc cctccctcaa tcggttgaat gtcgcccttt tgtctttggc gctggtaaac
     2101 catatgaatt ttctattgat tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc
     2161 ttttatatgt tgccaccttt atgtatgtat tttcgacgtt tgctaacata ctgcgtaata
     2221 aggagtctta ataagaattc tagaaataat tttgtttaac tttaagaagg agatatacat
     2281 atgaaatacc tgctgccgac cgctgcggcc ggcctgctgc tcctcgctgc ccagccggcg
     2341 atggccatgg gcgcgccggt accggctgtt ttggcggatg agagaagatt ttcagcctga
     2401 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     2461 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     2521 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     2581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2641 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     2701 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     2761 gatggccttt ttgcgtttct acaaactctt aagcttcgcc tgatgcggta ttttctcctt
     2821 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     2881 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2941 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3001 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3061 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     3121 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3181 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     3241 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3301 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     3361 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3421 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3481 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
