LOCUS       V016809                 5563 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016809
VERSION     V016809
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..759)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             986..1699
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      1774..1860
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1898..2647)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             2874..3452
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             3453..3485
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      complement(3509..3552)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4433..4527)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4561..5352)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
       61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
      121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
      181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
      241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
      301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
      361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
      421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
      481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
      541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
      601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
      661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
      721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
      781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
      841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
      901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
      961 attcattaaa gaggagaaag gtaccatgag caaaggagaa gaacttttca ctggagttgt
     1021 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tccgtggaga
     1081 gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca ctactggaaa
     1141 actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
     1201 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1261 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
     1321 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
     1381 agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat
     1441 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
     1501 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     1561 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
     1621 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     1681 catggatgag ctctacaaag cagcgaacga cgaaaattac gcccttgcag cgtaaaagct
     1741 taattagctg atctagacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa
     1801 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1861 ccgccgccct agacctagcc gtcttcacct cgagttaatt tttaaagtat gggcaatcaa
     1921 ttgctcctgt taaaattgct ttagaaatac tttggcagcg gtttgttgta ttgagtttca
     1981 tttgcgcatt ggttaaatgg aaagtgacag tacgctcact gcagcctaat atttttgaaa
     2041 tatcccaaga gctttttcct tcgcatgccc acgctaaaca ttctttttct cttttggtta
     2101 aatcgttgtt tgatttatta tttgctatat ttatttttcg ataattatca actagagaag
     2161 gaacaattaa tggtatgttc atacacgcat gtaaaaataa actatctata tagttgtctt
     2221 tttctgaatg tgcaaaacta agcattccga agccattgtt agccgtatga atagggaaac
     2281 taaacccagt gataagacct gatgttttcg cttctttaat tacatttgga gattttttat
     2341 ttacagcatt gttttcaaat atattccaat taattggtga atgattggag ttagaataat
     2401 ctactatagg atcatatttt attaaattag cgtcatcata atattgcctc cattttttag
     2461 ggtaattatc tagaattgaa atatcagatt taaccataga atgaggataa atgatcgcga
     2521 gtaaataata ttcacaatgt accattttag tcatatcaga taagcattga ttaatatcat
     2581 tattgcttct acaagcttta attttattaa ttattctgta tgtgtcgtcg gcatttatgt
     2641 ttttcatacc catctcttta tccttaccta ttgtttgtcg caagttttgc gtgttatata
     2701 tcattaaaac ggtaatggat tgacatttga ttctaataaa ttggattttt gtcacactat
     2761 tgtatcgctg ggaatacaat tacttaacat aagcacctgt aggatcgtac aggtttacgc
     2821 aagaaaatgg tttgttatag tcgaatgaat tcattaaaga ggagaaaggt accatgacta
     2881 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa
     2941 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc
     3001 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg
     3061 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg
     3121 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa
     3181 gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa
     3241 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa
     3301 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta
     3361 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta
     3421 ttaatgaaca gtttaaaaaa gcagtcttaa atgcagcgaa cgacgaaaat tacgcccttg
     3481 cagcgtaaac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     3541 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     3601 ctagacctag ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg actagtgctt ggattctcac
     4441 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     4501 tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca
     4561 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     4621 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     4681 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     4741 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     4801 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     4861 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     4921 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     4981 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     5041 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     5101 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     5161 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     5221 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     5281 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     5341 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
     5401 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     5461 accagagggc gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt
     5521 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
