LOCUS       V013569                 5569 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013569
VERSION     V013569
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..759)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             992..1705
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      1780..1866
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1904..2653)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             2880..3458
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             3459..3491
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      complement(3515..3558)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3763..4351)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4439..4533)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4567..5358)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
       61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
      121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
      181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
      241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
      301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
      361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
      421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
      481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
      541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
      601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
      661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
      721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
      781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
      841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
      901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
      961 attcattaaa gaggagaaag gatccggtac catgagcaaa ggagaagaac ttttcactgg
     1021 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtccg
     1081 tggagagggt gaaggtgatg ctacaaacgg aaaactcacc cttaaattta tttgcactac
     1141 tggaaaacta cctgttccgt ggccaacact tgtcactact ctgacctatg gtgttcaatg
     1201 cttttcccgt tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga
     1261 aggttatgta caggaacgca ctatatcttt caaagatgac gggacctaca agacgcgtgc
     1321 tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtattgattt
     1381 taaagaagat ggaaacattc ttggacacaa actcgagtac aactttaact cacacaatgt
     1441 atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa
     1501 cgttgaagat ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1561 tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgtcc tttcgaaaga
     1621 tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac
     1681 acatggcatg gatgagctct acaaagcagc gaacgacgaa aattacgccc ttgcagcgta
     1741 aaagcttaat tagctgatct agacgcgtgc tagaggcatc aaataaaacg aaaggctcag
     1801 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1861 acaaatccgc cgccctagac ctagccgtct tcacctcgag ttaattttta aagtatgggc
     1921 aatcaattgc tcctgttaaa attgctttag aaatactttg gcagcggttt gttgtattga
     1981 gtttcatttg cgcattggtt aaatggaaag tgacagtacg ctcactgcag cctaatattt
     2041 ttgaaatatc ccaagagctt tttccttcgc atgcccacgc taaacattct ttttctcttt
     2101 tggttaaatc gttgtttgat ttattatttg ctatatttat ttttcgataa ttatcaacta
     2161 gagaaggaac aattaatggt atgttcatac acgcatgtaa aaataaacta tctatatagt
     2221 tgtctttttc tgaatgtgca aaactaagca ttccgaagcc attgttagcc gtatgaatag
     2281 ggaaactaaa cccagtgata agacctgatg ttttcgcttc tttaattaca tttggagatt
     2341 ttttatttac agcattgttt tcaaatatat tccaattaat tggtgaatga ttggagttag
     2401 aataatctac tataggatca tattttatta aattagcgtc atcataatat tgcctccatt
     2461 ttttagggta attatctaga attgaaatat cagatttaac catagaatga ggataaatga
     2521 tcgcgagtaa ataatattca caatgtacca ttttagtcat atcagataag cattgattaa
     2581 tatcattatt gcttctacaa gctttaattt tattaattat tctgtatgtg tcgtcggcat
     2641 ttatgttttt catacccatc tctttatcct tacctattgt ttgtcgcaag ttttgcgtgt
     2701 tatatatcat taaaacggta atggattgac atttgattct aataaattgg atttttgtca
     2761 cactattgta tcgctgggaa tacaattact taacataagc acctgtagga tcgtacaggt
     2821 ttacgcaaga aaatggtttg ttatagtcga atgaattcat taaagaggag aaaggtacca
     2881 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
     2941 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
     3001 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
     3061 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
     3121 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
     3181 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
     3241 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
     3301 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
     3361 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     3421 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc agcgaacgac gaaaattacg
     3481 cccttgcagc gtaaacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     3601 gccgccctag acctagggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat
     4441 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     4501 ctgaggtcat tactggatct atcaacagga gtccaagcga gctctcgaac cccagagtcc
     4561 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     4621 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     4681 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     4741 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     4801 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     4861 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     4921 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     4981 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     5041 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     5101 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     5161 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     5221 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     5281 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     5341 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
     5401 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
     5461 aaccttacca gagggcgccc cagctggcaa ttccgacgtc taagaaacca ttattatcat
     5521 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
