LOCUS       V013477                 3684 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013477
VERSION     V013477
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..759)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             992..1741
                     /gene="aiiA"
                     /label="N-acyl homoserine lactonase"
                     /note="N-acyl homoserine lactonase from Bacillus
                     thuringiensis. Accession#: A3FJ64"
     CDS             1742..1774
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      1795..1881
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2050..2595)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2709..2803)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2827..3483)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3484..3586)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
       61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
      121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
      181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
      241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
      301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
      361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
      421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
      481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
      541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
      601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
      661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
      721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
      781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
      841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
      901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
      961 attcatcata tgtaaagagg agaaaggtac catgacagta aagaaacttt atttcatccc
     1021 agcaggtcgt tgcatgttgg atcattcgtc tgttaacagt gcgttaacac cggggaaact
     1081 attaaacttg ccggtgtggt gttatctttt ggagacggaa gaaggtccta ttttagtaga
     1141 cacaggtatg ccagaaagtg cagttaataa tgaagggctt tttaacggta catttgttga
     1201 aggacagatc ttaccgaaaa tgactgaaga agatagaatc gtgaatatat taaagcgtgt
     1261 agggtatgag ccggacgacc ttttatatat tattagttct cacttacatt ttgatcatgc
     1321 aggaggaaac ggtgctttta caaatacacc aattattgtg cagcgaacgg aatatgaggc
     1381 agcacttcat agagaagaat atatgaaaga atgtatatta ccgcatttga actacaaaat
     1441 tattgaaggg gattatgaag tggtaccagg tgttcaatta ttgtatacgc caggtcattc
     1501 tccaggccat cagtcgctat tcattgagac ggagcaatcc ggttcagttt tattaacgat
     1561 tgatgcatcg tacacgaaag agaattttga agatgaagtg ccgttcgcag gatttgatcc
     1621 agaattagct ttatcttcaa ttaaacgttt aaaagaagtt gtgaaaaaag agaaaccaat
     1681 tattttcttt ggtcatgata tagagcagga aaagagttgt agagtgttcc cggaatatat
     1741 agcagcgaac gacgaaaatt acgcccttgc agcgtaaacg cgtgctagag gcatcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1861 gctctcctga gtaggacaaa tccgccgccc tagacctagg gatatattcc gcttcctcgc
     1921 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     1981 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     2041 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
     2101 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
     2161 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     2221 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     2281 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
     2341 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
     2401 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
     2461 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     2521 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     2581 ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg
     2641 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgttacta
     2701 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2761 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2821 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     2881 gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2941 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     3001 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     3061 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     3121 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     3181 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     3241 ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat
     3301 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     3361 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     3421 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     3481 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     3541 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     3601 ccagatatcg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3661 atcacgaggc cctttcgtct tcac
//
