LOCUS       62056_21045       14317 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          10..199
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             194..2017
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2024..2041
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2076..2328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2350..2374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3675..4301
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4733..5803
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5872..6066
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6410..6550
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6736..7324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7414..8205)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8630..8654
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8732..8906)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8949..9665)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(9732..10409)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10601..10622
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10637..10667
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10675..10691
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10699..10715
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10732..10984)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(11549..13541)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     promoter        13950..14295
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
       61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
      121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
      181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
      241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
      301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
      361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
      421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
      481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
      541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
      601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
      661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
      721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
      781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
      841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
      901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
      961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
     1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac
     1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
     1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
     1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
     1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
     1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
     1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
     1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
     2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2401 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2461 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2521 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     2581 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2641 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2701 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     2761 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     2821 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     2881 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     2941 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     3001 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     3061 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     3121 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3181 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3241 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3301 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3361 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3421 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     3481 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3541 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3601 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     3661 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3721 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     3781 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     3841 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     3901 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     3961 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     4021 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     4081 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     4141 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4201 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4261 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4321 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4381 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4441 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4501 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4561 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4621 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4681 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     4741 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     4801 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     4861 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     4921 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     4981 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     5041 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     5101 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     5161 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     5221 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     5281 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     5341 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     5401 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     5461 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     5521 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     5581 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     5641 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     5701 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     5761 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     5821 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     5881 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     5941 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     6001 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     6061 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     6121 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     6181 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     6241 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     6301 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6361 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6421 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6481 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6541 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6661 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6901 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6961 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7021 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7081 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7141 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7201 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7261 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7321 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7381 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
     7441 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
     7501 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
     7561 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
     7621 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
     7681 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
     7741 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
     7801 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
     7861 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
     7921 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
     7981 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
     8041 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
     8101 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
     8161 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
     8221 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     8281 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     8341 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     8401 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     8461 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     8521 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     8581 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     8641 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
     8701 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
     8761 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
     8821 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
     8881 actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc cggtcggcat
     8941 ctacttcact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     9001 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     9061 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     9121 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     9181 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     9241 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     9301 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     9361 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     9421 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     9481 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     9541 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     9601 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     9661 accatatctc attgcccccc gggatctgcg aaagctcgag gagatagatt tgtagagaga
     9721 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt
     9781 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     9841 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     9901 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     9961 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    10021 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    10081 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    10141 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    10201 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    10261 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    10321 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    10381 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    10441 tgatattctt ggagtagacg agagtgtcgt gctccaccat gtttggcaag ctgctctagc
    10501 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10561 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10621 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10681 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattcc cgatctagta
    10741 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
    10801 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
    10861 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
    10921 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
    10981 gatcggggaa attcgagctc tctagaacta gtatcgatct gaaaatctcg aaacagccgt
    11041 gtcatagtca atcattaggt gttataggaa caatcaaagg ttttttcaag tgttaatctt
    11101 catactaata tatacagtgg gtactcttta tctactgccg tggaactgtc atatttgatt
    11161 atgaaatttt agctctagaa aatatttgat catcaatgtc aagactttat gaccttgcaa
    11221 aatacatttc ctaattgaga acagggtaaa attatgaact atgcctctga accttcatac
    11281 acaggcagca cattttttgt tgtaaaattc atcttaatat cagcggaaag actggaccag
    11341 agaaagaaaa agttaagaca ggcatatact cttgatcctc taaaagagat gaggcggtac
    11401 aatgatcaac catgaacatt aaagtgatac gtggaacatg agaacacaaa taattgtcac
    11461 tggaacataa gtacaaaaag gtttgtagta acttaccgct agcagatctg tcgacctcga
    11521 gggtacccgg ggatcctcta gagtcgacct gcagaagtaa caccaaacaa cagggtgagc
    11581 atcgacaaaa gaaacagtac caagcaaata aatagcgtat gaaggcaggg ctaaaaaaat
    11641 ccacatatag ctgctgcata tgccatcatc caagtatatc aagatcaaaa taattataaa
    11701 acatacttgt ttattataat agataggtac tcaaggttag agcatatgaa tagatgctgc
    11761 atatgccatc atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat
    11821 cgatatttcc atccatctta aactcgtaac tatgaagatg tatgacacac acatacagtt
    11881 ccaaaattaa taaatacacc aggtagtttg aaacagtatt ctactccgat ctagaacgaa
    11941 tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag catctctgta
    12001 tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata tttccatcca
    12061 tcatcttcaa ttcgtaacta tgaatatgta tggcacacac atacagatcc aaaattaata
    12121 aatccaccag gtagtttgaa acagaattct actccgatct agaacgaccg cccaaccaga
    12181 ccacatcatc acaaccaaga caaaaaaaag catgaaaaga tgacccgaca aacaagtgca
    12241 cggcatatat tgaaataaag gaaaagggca aaccaaaccc tatgcaacga aacaaaaaaa
    12301 atcatgaaat cgatcccgtc tgcggaacgg ctagagccat cccaggattc cccaaagaga
    12361 aacactggca agttagcaat cagaacgtgt ctgacgtaca ggtcgcatcc gtgtacgaac
    12421 gctagcagca cggatctaac acaaacacgg atctaacaca aacatgaaca gaagtagaac
    12481 taccgggccc taaccatgga ccggaacgcc gatctagaga aggtagagag gggggggggg
    12541 ggaggacgag cggcgtacct tgaagcggag gtgccgacgg gtggatttgg gggagatctg
    12601 gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga aagagggtgt ggagggggtg
    12661 tctatttatt acggcgggcg aggaagggaa agcgaaggag cggtgggaaa ggaatccccc
    12721 gtagctgccg gtgccgtgag aggaggagga ggccgcctgc cgtgccggct cacgtctgcc
    12781 gctccgccac gcaatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact
    12841 ctcgagaggg gtccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga
    12901 cgcgacgctg ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc
    12961 tggcattatc tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg
    13021 tatttgttta atttttagtc actttatttt attctatttt atatctaaat tattaaataa
    13081 aaaaactaaa atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat
    13141 gtactaaaaa aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat
    13201 aaaatggatg aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca
    13261 cactaaaaag ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc ctttagacca
    13321 tgtctaactg ttcatttata tgattctcta aaacactgat attattgtag tactatagat
    13381 tatattattc gtagagtaaa gtttaaatat atgtataaag atagataaac tgcacttcaa
    13441 acaagtgtga caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt
    13501 atctctagag aggggcacga ccgggtcacg ctgcactgca ggcatgcaag cttggcactg
    13561 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    13621 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    13681 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
    13741 gtcgtttccc gccttcagtt tagcttcatg gagtcaaaga ttcaaataga ggacctaaca
    13801 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
    13861 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
    13921 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
    13981 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
    14041 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
    14101 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
    14161 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
    14221 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
    14281 catttggaga gaacacgggg gactcttgac catggta
//
