LOCUS       40924_42739        8811 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTCCR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Wu G, Wang Y, Zhu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, 
            Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
            430072, People's Republic of China
REFERENCE   2  (bases 1 to 8811)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese 
            Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
            People's Republic of China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(419..452)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(465..586)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(710..1387)
                     /codon_start=1
                     /label=DsRed1
                     /note="wild-type DsRed"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     promoter        complement(1469..1672)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1673..1976)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      complement(2074..2321)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(2390..2761)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2780..2827)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     terminator      complement(2880..2927)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3069..4097)
                     /codon_start=1
                     /label=Cre
                     /note="site-specific recombinase"
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    complement(4103..4680)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     protein_bind    complement(4719..4743)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4744..4762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5117..5138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5154..6233)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(6234..6311)
                     /label=lacI promoter
     protein_bind    complement(6520..6553)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6590..6606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6614..6630)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6638..6668)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6683..6704)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6992..7580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7754..8611)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8612..8716)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggta
      421 ccgttcgtat agcatacatt atacgaagtt atggatctac gccttaagat acattgatga
      481 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      541 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      601 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      661 cctctacaaa tgtggtatgg ctgattatga tctagagtcg cggccgctac aggaacaggt
      721 ggtggcggcc ctcggtgcgc tcgtactgct ccacgatggt gtagtcctcg ttgtgggagg
      781 tgatgtccag cttggagtcc acgtagtagt agccgggcag ctgcacgggc ttcttggcca
      841 tgtagatgga cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggccttgt
      901 ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc
      961 ccatggtctt cttctgcatt acggggccgt cggaggggaa gttcacgccg atgaacttca
     1021 ccttgtagat gaagcagccg tcctgcaggg aggagtcttg ggtcacggtc accacgccgc
     1081 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt
     1141 agtcggggat gtcggcgggg tgcttcacgt acaccttgga gccgtactgg aactgggggg
     1201 acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttcacggtgt
     1261 tgtggccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
     1321 cggtgccctc catgcgcacc ttgaagcgca tgaactcctt gatgacgttc ttggaggagc
     1381 gcaccatggt ggcgaccggt ggatctcata tgcctaggag ctccgcggac gtcgacaagc
     1441 tgatcccgat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
     1501 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     1561 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     1621 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     1681 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1741 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1801 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1861 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     1921 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     1981 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactaaagc
     2041 tgatcttttt gctcacatgt tggtctccag cttgcaaatt aaagccttcg agcgtcccaa
     2101 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
     2161 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
     2221 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
     2281 gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa aggaaaaggg
     2341 ggacggatct ccgaggcctg ggacccgtgg gccgccgtcg gacgtgtcag tcctgctcct
     2401 cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg
     2461 gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga
     2521 cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt
     2581 tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca
     2641 caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact
     2701 cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca
     2761 tggtttagtt cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat
     2821 tgtcaacacc gcccttagat tagattgcta tgcggatctg atatagttcc tcctttcagc
     2881 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
     2941 gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc gtcgacttaa
     3001 ttaattaggc ctctcgagct gcagggatca attccagcac actggcggcc gttactagtg
     3061 gatctctaat cgccatcttc cagcaggcgc accattgccc ctgtttcact atccaggtta
     3121 cggatatagt tcatgacaat atttacattg gtccagccac cagcttgcat gatctccggt
     3181 attgaaactc cagcgcgggc catatctcgc gcggctccga cacgggcact gtgtccagac
     3241 caggccaggt atctctgacc agagtcatcc ttagcgccgt aaatcaatcg atgagttgct
     3301 tcaaaaatcc cttccagggc gcgagttgat agctggctgg tggcagatgg cgcggcaaca
     3361 ccattttttc tgacccggca aaacaggtag ttattcggat catcagctac accagagacg
     3421 gaaatccatc gctcgaccag tttagttacc cccaggctaa gtgccttctc tacacctgcg
     3481 gtgctaacca gcgttttcgt tctgccaata tggattaaca ttctcccacc gtcagtacgt
     3541 gagatatctt taaccctgat cctggcaatt tcggctatac gtaacagggt gttataagca
     3601 atccccagaa atgccagatt acgtatatcc tggcagcgat cgctattttc catgagtgaa
     3661 cgaacctggt cgaaatcagt gcgttcgaac gctagagcct gttttgcacg ttcaccggca
     3721 tcaacgtttt cttttcggat ccgccgcata accagtgaaa cagcattgct gtcacttggt
     3781 cgtggcagcc cggaccgacg atgaagcatg tttagctggc ccaaatgttg ctggatagtt
     3841 tttactgcca gaccgcgcgc ctgaagatat agaagataat cgcgaacatc ttcaggttct
     3901 gcgggaaacc atttccggtt attcaacttg caccatgccg cccacgaccg gcaaacggac
     3961 agaagcattt tccaggtatg ctcagaaaac gcctggcgat ccctgaacat gtccatcagg
     4021 ttcttgcgaa cctcatcact cgttgcatcg accggtaatg caggcaaatt ttggtgtacg
     4081 gtcagtaaat tggccataga tcccatggta ttatcgtgtt tttcaaagga aaaccacgtc
     4141 cccgtggttc ggggggccta gacgtttttt aacctcgact aaacacatgt aaagcatgtg
     4201 caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc
     4261 ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca actcacaacg
     4321 tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag
     4381 aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt
     4441 tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag accttgcatt
     4501 cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg gccaggtttc
     4561 cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata gacaaacgca
     4621 caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga gggagagggg
     4681 cggaattgat cccgctagag ggaaaccgtt gtggtctcgg aattgttatc cgctcacaat
     4741 tcccctatag tgagtcgtat taatttcgcg ggatcgagat caattcaaca atgtctggaa
     4801 agaactgtcc ttcatcgggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
     4861 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaatagcgca
     4921 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     4981 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     5041 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     5101 atcggtcgag tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5221 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     5281 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     5341 tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg gcgggatata acatgagctg
     5401 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     5461 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     5521 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     5581 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     5641 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     5701 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     5761 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     5821 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     5881 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     5941 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     6001 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     6061 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     6121 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     6181 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     6241 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     6301 tcgatggtgt ctcgacgctc tcccttatgc gactcctgca ctagatattg agtgatttgc
     6361 aatatatcag ataatgaaga ttcatcatct tgactagcca aatacttaaa aaatgaatca
     6421 tcatctgcga agaacatcgt taagagatac tggttgtgat ccatttattg atcgcaaaag
     6481 cttgtcgacg tccgcggagc tcctaggcat atgagatcca taacttcgta tagcatacat
     6541 tatacgaacg gtagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
     6601 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6661 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8761 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
