LOCUS       V002799                12404 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002799
VERSION     V002799
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Wu G, Wang Y, Zhu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology,
            Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
            430072, People's Republic of China
REFERENCE   2  (bases 1 to 12404)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese
            Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
            People's Republic of China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(419..452)
                     /label="lox71"
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(465..586)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(710..1387)
                     /label="DsRed1"
                     /note="wild-type DsRed"
     promoter        complement(1469..1672)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1673..1976)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      complement(2074..2321)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(2390..2761)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(2780..2827)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     regulatory      complement(2860..2990)
                     /label="T7 terminator"
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     terminator      2880..2927
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(3069..4097)
                     /label="Cre"
                     /note="site-specific recombinase"
     misc_feature    complement(4109..4683)
                     /note="internal ribosome entry sequence from
                     encephalomyocarditis virus"
     misc_feature    4109..4680
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     protein_bind    4719..4743
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4744..4762)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(4745..4762)
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     terminator      complement(4857..4904)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(5012..7660)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(7670..7690)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    complement(7696..8273)
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     protein_bind    complement(8312..8336)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8337..8355)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8710..8731
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8747..9826)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(9827..9904)
                     /label="lacI promoter"
     protein_bind    complement(10113..10146)
                     /label="lox66"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10183..10199)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    10207..10223
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10231..10261)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10276..10297)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10585..11173)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11347..12204)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12205..12309)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggta
      421 ccgttcgtat agcatacatt atacgaagtt atggatctac gccttaagat acattgatga
      481 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      541 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      601 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      661 cctctacaaa tgtggtatgg ctgattatga tctagagtcg cggccgctac aggaacaggt
      721 ggtggcggcc ctcggtgcgc tcgtactgct ccacgatggt gtagtcctcg ttgtgggagg
      781 tgatgtccag cttggagtcc acgtagtagt agccgggcag ctgcacgggc ttcttggcca
      841 tgtagatgga cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggccttgt
      901 ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc
      961 ccatggtctt cttctgcatt acggggccgt cggaggggaa gttcacgccg atgaacttca
     1021 ccttgtagat gaagcagccg tcctgcaggg aggagtcttg ggtcacggtc accacgccgc
     1081 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt
     1141 agtcggggat gtcggcgggg tgcttcacgt acaccttgga gccgtactgg aactgggggg
     1201 acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttcacggtgt
     1261 tgtggccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
     1321 cggtgccctc catgcgcacc ttgaagcgca tgaactcctt gatgacgttc ttggaggagc
     1381 gcaccatggt ggcgaccggt ggatctcata tgcctaggag ctccgcggac gtcgacaagc
     1441 tgatcccgat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
     1501 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     1561 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     1621 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     1681 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1741 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1801 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1861 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     1921 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     1981 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactaaagc
     2041 tgatcttttt gctcacatgt tggtctccag cttgcaaatt aaagccttcg agcgtcccaa
     2101 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
     2161 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
     2221 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
     2281 gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa aggaaaaggg
     2341 ggacggatct ccgaggcctg ggacccgtgg gccgccgtcg gacgtgtcag tcctgctcct
     2401 cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg
     2461 gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga
     2521 cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt
     2581 tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca
     2641 caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact
     2701 cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca
     2761 tggtttagtt cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat
     2821 tgtcaacacc gcccttagat tagattgcta tgcggatctg atatagttcc tcctttcagc
     2881 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
     2941 gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc gtcgacttaa
     3001 ttaattaggc ctctcgagct gcagggatca attccagcac actggcggcc gttactagtg
     3061 gatctctaat cgccatcttc cagcaggcgc accattgccc ctgtttcact atccaggtta
     3121 cggatatagt tcatgacaat atttacattg gtccagccac cagcttgcat gatctccggt
     3181 attgaaactc cagcgcgggc catatctcgc gcggctccga cacgggcact gtgtccagac
     3241 caggccaggt atctctgacc agagtcatcc ttagcgccgt aaatcaatcg atgagttgct
     3301 tcaaaaatcc cttccagggc gcgagttgat agctggctgg tggcagatgg cgcggcaaca
     3361 ccattttttc tgacccggca aaacaggtag ttattcggat catcagctac accagagacg
     3421 gaaatccatc gctcgaccag tttagttacc cccaggctaa gtgccttctc tacacctgcg
     3481 gtgctaacca gcgttttcgt tctgccaata tggattaaca ttctcccacc gtcagtacgt
     3541 gagatatctt taaccctgat cctggcaatt tcggctatac gtaacagggt gttataagca
     3601 atccccagaa atgccagatt acgtatatcc tggcagcgat cgctattttc catgagtgaa
     3661 cgaacctggt cgaaatcagt gcgttcgaac gctagagcct gttttgcacg ttcaccggca
     3721 tcaacgtttt cttttcggat ccgccgcata accagtgaaa cagcattgct gtcacttggt
     3781 cgtggcagcc cggaccgacg atgaagcatg tttagctggc ccaaatgttg ctggatagtt
     3841 tttactgcca gaccgcgcgc ctgaagatat agaagataat cgcgaacatc ttcaggttct
     3901 gcgggaaacc atttccggtt attcaacttg caccatgccg cccacgaccg gcaaacggac
     3961 agaagcattt tccaggtatg ctcagaaaac gcctggcgat ccctgaacat gtccatcagg
     4021 ttcttgcgaa cctcatcact cgttgcatcg accggtaatg caggcaaatt ttggtgtacg
     4081 gtcagtaaat tggccataga tcccatggta ttatcgtgtt tttcaaagga aaaccacgtc
     4141 cccgtggttc ggggggccta gacgtttttt aacctcgact aaacacatgt aaagcatgtg
     4201 caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc
     4261 ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca actcacaacg
     4321 tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag
     4381 aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt
     4441 tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag accttgcatt
     4501 cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg gccaggtttc
     4561 cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata gacaaacgca
     4621 caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga gggagagggg
     4681 cggaattgat cccgctagag ggaaaccgtt gtggtctcgg aattgttatc cgctcacaat
     4741 tcccctatag tgagtcgtat taatttcgcg ggatcgagat caattcaaca atgtcagatc
     4801 aattccagca cactggcggc cgttactagt ggatctgata tagttcctcc tttcagcaaa
     4861 aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg
     4921 gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcgtc gaaattccag
     4981 cacactggcg gccgttacta gtggatcttt acgcgaacgc gaagtccgac tctaagatgt
     5041 cacggaggtt caagttacct ttagccggaa gtgctggcat tttgtccaat tgagactcgt
     5101 gcaactggtc agcgaactgg tcgtagaaat cagccagtac atcacaagac tcatatgtgt
     5161 caaccatagt ttcgcgcact gctttgaaca ggttcgcagc gtcagccgga atggtaccga
     5221 aggagtcgtg aatcagtgca aaagattcga ttccgtactt ctcgtgtgcc cacactacag
     5281 tcttacgaag gtggctaccg tcttggctgt gtacaaagtt aggagcgata ccagactcct
     5341 gtttgtgtgc atcaatctcg ctatctttgt tggtgttaat ggtaggctgt aagcggaact
     5401 gaccgaggaa catcaggttc aagcgcgtct gaataggctt cttgtattcc tgccacacag
     5461 ggaaaccatc aggagttacc caatgcacag cgcaacgctt gcgaagaatc tctccagtct
     5521 tcttatcttt gacctcagca gccagcagct tagcagcaga cttaagccag ttcattgctt
     5581 caaccgcagc taccaccgtc acgctcacag attcccaaat cagcttagcc atgtatccag
     5641 cagcctgatt cggctgagtg aacatcagac ccttgccgga atcaatagct ggctgaatgg
     5701 tatcttccag cacttgttga cggaagccga actctttgga cccgtaagcc agcgtcatga
     5761 ctgaacgctt agtcacactg cgagtaacac cgtaagccag ccattgacca gccagtgcct
     5821 tagtgcccag cttgactttc tcagagattt caccagtgtt ctcatcggtc acggtaacta
     5881 cttcgttatc ggtcccattg attgcgtctg cttgtagaat ctcgttgact ttcttagcaa
     5941 caatcccgta gatgtcctga acggtttcac taggaagcaa gttaaccgcg cgaccaccta
     6001 cctcatctcg gagcatcgcg gagaagtgct ggatgccaga gcaagacccg tcaaacgcca
     6061 gcggaaggga gcagttatag ctcaggccgt ggtgctgtac cccagcgtac tcaaagcaga
     6121 acgcaaggaa gcagaacgga gaatcttgct cagcccacca agtgttctcc agtggagact
     6181 tagcgcaagc catgatgttc tcgtggtttt cctcaatgaa cttgatgcgc tcagggaacg
     6241 gaaccttatc gacacccgca cagtttgcac cgtggatttt cagccagtag taaccttcct
     6301 taccgattgg tttacctttc gccagcgtaa gcagtccttt ggtcatatcg ttaccttgcg
     6361 ggttgaacat tgacacagcg taaacacgac cgcgccagtc catgttgtaa gggaaccaga
     6421 tggccttatg gttagcaaac ttattggctt gctcaagcat gaactcaagg ctgatacggc
     6481 gagacttgcg agccttgtcc ttgcggtaca cagcagcggc agcacgtttc cacgcggtga
     6541 gagcctcagg attcatgtcg atgtcttccg gtttcatcgg gagttcttca cgctcaatcg
     6601 cagggatgtc ctcgaccgga caatgcttcc acttggtgat tacgttggcg accgctagga
     6661 ctttcttgtt gattttccat gcggtgtttt gcgcaatgtt aatcgctttg tacacctcag
     6721 gcatgtaaac gtcttcgtag cgcatcagtg ctttcttact gtgagtacgc accagcgcca
     6781 gaggacgacg accgttagcc caatagccac caccagtaat gccagtccac ggcttaggag
     6841 gaactacgca aggttggaac atcggagaga tgccagccag cgcacctgca cgggttgcga
     6901 tagcctcagc gtattcaggt gcgagttcga tagtctcaga gtcttgacct actacgccag
     6961 cattttggcg gtgtaagcta accattccgg ttgactcaat gagcatctcg atgcagcgta
     7021 ctcctacatg aatagagtct tccttatgcc acgaagacca cgcctcgcca ccgagtagac
     7081 ccttagagag catgtcagcc tcgacaactt gcataaatgc tttcttgtag acgtgcccta
     7141 cgcgcttgtt gagttgttcc tcaacgtttt tcttgaagtg cttagcttca aggtcacgga
     7201 tacgaccgaa gcgagcctcg tcctcaatgg cccgaccgat tgcgcttgct acagcctgaa
     7261 cggttgtatt gtcagcactg gttaggcaag ccagagtggt cttaatggtg atgtacgcta
     7321 cggcttccgg cttgatttct tgcaggaact ggaaggctgt cgggcgcttg ccgcgcttag
     7381 ctttcacttc ctcaaaccag tcgttgatgc gtgcaatcat cttagggagt agggtagtga
     7441 tgagaggctt ggcggcagcg ttatccgcaa cctcaccagc tttaagttga cgctcaaaca
     7501 tcttgcggaa gcgtgcttca cccatctcgt aagactcatg ctcaagggcc aactgttcgc
     7561 gagctaaacg ctcaccgtaa tggtcagcca gagtgttgaa cgggatagca gccagttcga
     7621 tgtcagagaa gtcgttctta gcgatgttaa tcgtgttcat agatccgatc acctttctct
     7681 tcttcttagg agatcccatg gtattatcgt gtttttcaaa ggaaaaccac gtccccgtgg
     7741 ttcggggggc ctagacgttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag
     7801 gccccagatc agatcccata caatggggta ccttctgggc atccttcagc cccttgttga
     7861 atacgcttga ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact
     7921 ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc
     7981 tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc
     8041 aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg
     8101 cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc
     8161 tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc
     8221 cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggagag gggcggaatt
     8281 gatcccgcta gagggaaacc gttgtggtct cggaattgtt atccgctcac aattccccta
     8341 tagtgagtcg tattaatttc gcgggatcga gatcaattca acaatgtctg gaaagaactg
     8401 tccttcatcg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     8461 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatagc gcaagcgaca
     8521 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     8581 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     8641 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     8701 gagtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8761 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8821 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     8881 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     8941 gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
     9001 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     9061 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     9121 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     9181 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     9241 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     9301 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     9361 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     9421 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     9481 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     9541 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     9601 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     9661 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     9721 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     9781 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     9841 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     9901 tgtctcgacg ctctccctta tgcgactcct gcactagata ttgagtgatt tgcaatatat
     9961 cagataatga agattcatca tcttgactag ccaaatactt aaaaaatgaa tcatcatctg
    10021 cgaagaacat cgttaagaga tactggttgt gatccattta ttgatcgcaa aagcttgtcg
    10081 acgtccgcgg agctcctagg catatgagat ccataacttc gtatagcata cattatacga
    10141 acggtagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
    10201 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10261 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10321 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    12361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
