LOCUS       40924_42669        4711 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pTAPKan1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Spirek M, Gregan J, Chen Z.
  TITLE     Yeast tagging vector pTAPKan1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Spirek M, Gregan J, Chen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2008) Department of Chromosome Biology, University
            of Vienna, Viehmarktgasse 2a, Wien 1030, Austria
REFERENCE   3  (bases 1 to 4711)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-2008) Department of Chromosome Biology, University of 
            Vienna, Viehmarktgasse 2a, Wien 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             85..162
                     /codon_start=1
                     /label=CBP
                     /note="calmodulin-binding peptide"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             196..216
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             238..258
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             313..486
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     terminator      714..901
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            976..2332
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2437..2455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2713..3301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3475..4332)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4333..4437)
                     /label=AmpR promoter
ORIGIN
        1 caccatatga tcaccggtac cctaggatcc agcgctagcg ctgtcgacgg tcttaaggcg
       61 cccgggttaa ttaaatctca cgaaaagaga agatggaaga agaacttcat tgctgtttct
      121 gctgctaaca gattcaagaa gatttcctct tctggtgctt tggactacga catttctaga
      181 ccaactactg cctctgaaaa tttgtacttt caaggcgagt tgaagaccac tgcttctgaa
      241 aacttgtact tccaaggtga gttactcgag ggtgaattga agactgctgc tttggctcaa
      301 cacgacgaag ctgttgataa taagttcaat aaggaacagc aaaacgcatt ctacgagatt
      361 ttgcatttgc caaatttgaa cgaggagcaa cgtaacgctt ttattcaatc cttgaaggat
      421 gacccatccc aatctgccaa cttgttagct gaagccaaga agttaaacga cgctcaagcc
      481 ccaaaagttg acaataagtt taacaaggaa caacaaaacg ccttttacga aattttgcac
      541 ttgccaaact tgaacgaaga acaaagaaac gctttcattc aatctttgaa ggacgaccca
      601 tctcaatctg ctaatttgtt ggctgaagct aagaagttga acggtgctca agctccaaag
      661 gttgacgcta actctgctgg taagtctact tgaggcgcgc cacttctaaa taagcgaatt
      721 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      781 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      841 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
      901 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg
      961 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     1021 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
     1081 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     1141 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
     1201 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
     1261 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
     1321 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt
     1381 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     1441 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1501 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     1561 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     1621 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1681 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1741 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1801 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1861 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1921 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1981 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2041 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2101 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt
     2161 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     2221 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     2281 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     2341 tcgatactaa cgccgccatc cagtttaaac gagctcgaat tcatcgatga tatcagatcc
     2401 actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc
     2461 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2881 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3121 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4681 tgcggcatca gagcagattg tactgagagt g
//
