LOCUS       40924_42664        6479 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTAP6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Dodsworth JA, Li L, Wei S, Hedlund BP, Leigh JA, de Figueiredo P.
  TITLE     Interdomain conjugal transfer of DNA from bacteria to archaea
  JOURNAL   Appl. Environ. Microbiol. 76 (16), 5644-5647 (2010)
  PUBMED    20581182
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Dodsworth JA, Li L, Wei S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2010) Plant Pathology and Microbiology, Texas A
REFERENCE   3  (bases 1 to 6479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "16"; 
            pages: "5644-5647"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2010) Plant Pathology and Microbiology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(971..1567)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     oriT            complement(2597..2706)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(2913..2931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2938..2954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3095..3523
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3867..4658
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4679..5536
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5710..6298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgggcc ccccctcgag cttttacaac gaaatttccg ccttttttca acattttggt
      301 tgccgtcatg agcgccattg ttgtaagttc aagggacctt acgtgatcca catcccaaac
      361 cccactgatg tttggggaag catcgcagat tataacatct ggtttttctg gtaaaaggtc
      421 ttttgcctgt tttaatattt cttcttttgt catatcgcct ttaattgcaa tgatattgtc
      481 atgcgggagt ggttttacag tttgtaaatc aattccaacg ataaatccat cttccccaac
      541 aatatctctt gcagcttgga gccatcctcc aggagcacac cctaaatcta caacaacgtt
      601 tctttctttt attaaattga atttttcatt taactggaat aatttatacg ttgctcttga
      661 tctgtaattt cttctttttg ctaaattata gtaatggtcg tttttccttt gaagaaccca
      721 tcttttgtcc ttttttccca taattatctc cttatattaa aaaatttaaa tatcgtcgta
      781 atgtgttagt tctgaatagt caattgattt gtggagggtc ttcatttcca cagggatgtt
      841 atttttgcgt atatttgcaa atgggtttgt tccacctatt gtggtaattc caaacataca
      901 tttttcaaca cgaacaccgc agatgtcgtt atttggcctt cctatttcga ttatcgaatt
      961 ctgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt cgcgcggtcc ttcgggcact
     1021 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     1081 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     1141 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     1201 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     1261 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     1321 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     1381 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     1441 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     1501 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     1561 cggtcatgag aatcactcct atttttttga tatatacatc ataacattac tctatgtata
     1621 tatattcact ttttcattaa cattaaatag aaaagtttat atataagatg ttaataacac
     1681 aataatttga atttgaatac tcaaaaaatg ggctttaata tataaaatta agatgaaaat
     1741 agatgatttt ttaaaaaaat gttattatta tatctcaata tctaaatatt agattaatat
     1801 taattattac ccaaatattt caatgaatat ttagttttga atagtatatt acgaataggg
     1861 cgttttttat tacctactac tattttccga agatttttta agactctcga tatcataacc
     1921 cctgtctttt agtgaatctg caattatttt tgcaccaact ggggcagatg cttcagataa
     1981 aatggataaa atttcaacat caatattact gtccataatg ccacacctat taacctaaaa
     2041 acttaaaaaa agttattttt taagattcct atatcctgaa gagatcaatg cagcaccaac
     2101 tgcaccgata tattgagaat attttggaac tattattttt ttaccgagta tttcttcaag
     2161 tgcaagaacc attcctttca aaagactgct tccaccaacc aaaattaaag gatcccttac
     2221 atcaacttct tggagttgct gttcgtaaat ttgttctgca acagagtgag cagctgcggc
     2281 tgcaacatca tttggcgtag caccgcctgc aagaccggtt actaaatcct ggatcccaaa
     2341 tacaatacag tagctgttca tcataacgtt tcgccagtct cccgttgctg caatgtcacc
     2401 aagttcctga atggataccc caagtcttct tgcagtaatt tcaaagaatc taccgcttgc
     2461 accggcacag attcccccca ttgtgaaacc atctggaatt gcattgttca gcgaaattgc
     2521 ttttctagag cggccgccac cgcggtggag ctcggatcca ctagtaacgg ccgccagtgt
     2581 gctggaattc gcccttccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     2641 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     2701 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     2761 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     2821 cagggttatg cagcggaaaa gcaagggcga attctgcaga tatccatcac actggcggcc
     2881 gctcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact
     2941 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3001 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3061 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     3121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3361 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3421 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3481 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc
     3541 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
     3601 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     3661 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     3721 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     3781 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc
     3841 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     3901 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3961 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4021 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4081 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4141 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga
     4201 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4261 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4321 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4381 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4441 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4501 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4561 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4621 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat
     4681 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4741 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4801 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4861 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4921 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4981 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5041 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5101 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5161 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5221 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5281 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5341 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5401 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5461 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5521 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5581 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5641 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5701 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5761 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5821 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5881 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5941 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6001 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6061 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6121 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6181 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6241 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6301 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6361 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6421 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
