LOCUS       40924_42609        4956 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             226..1875
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1919..2040)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2459..3047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3221..4078)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4079..4183)
                     /label=AmpR promoter
     rep_origin      4210..4665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4796..4844
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4858..4949
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgccgccccg actgcatctg
       61 cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca tagaactaaa
      121 gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac gggccacggg
      181 gatgaagcag aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa
      241 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      301 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      361 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      421 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      481 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
      541 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
      601 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
      661 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
      721 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
      781 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
      841 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
      901 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
      961 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     1021 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     1081 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     1141 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     1201 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     1261 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     1321 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     1381 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     1441 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     1501 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     1561 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     1621 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     1681 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     1741 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     1801 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     1861 ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1921 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1981 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2041 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2101 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     2161 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     2221 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     2281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2881 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4201 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4261 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4501 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4561 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4621 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca
     4681 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
     4741 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
     4801 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     4861 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     4921 tgcaagtgca ggtgccagaa catttctcta tcgata
//
