LOCUS       40924_42409        4871 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..79
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1790
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1834..1955)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2374..2962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3136..3993)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3994..4098)
                     /label=AmpR promoter
     rep_origin      4125..4580
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4711..4759
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4773..4864
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tagactctag agggtatata
       61 atggaagctc gaattccagc ttggcattcc ggtactgttg gtaaaaagct tggcattccg
      121 gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
      181 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
      241 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
      301 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
      361 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
      421 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
      481 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
      541 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
      601 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
      661 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
      721 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
      781 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
      841 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
      901 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
      961 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     1021 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     1081 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     1141 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     1201 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     1261 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     1321 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     1381 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     1441 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     1501 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     1561 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     1621 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     1681 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     1741 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga
     1801 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     1861 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1921 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1981 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     2041 gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     2101 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     2161 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     2221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2281 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2341 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2401 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2461 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2521 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2581 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2641 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2701 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2761 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2821 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2881 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2941 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3001 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3061 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3121 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3181 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3241 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3301 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3361 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3421 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3481 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3541 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3601 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3661 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3721 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3781 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3841 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3901 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3961 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4021 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4081 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     4141 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4201 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4261 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4321 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4381 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4441 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4501 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4561 aatattaacg tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4621 atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat
     4681 taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc attacatctg
     4741 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     4801 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     4861 ctctatcgat a
//
