LOCUS       pT7Blue-2.        3103 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Multi-purpose cloning vector with T7 and SP6 promoters, and with a 
            multiple cloning site that encodes a cleavable N-terminal S-tag.
ACCESSION   .
VERSION     .
KEYWORDS    pT7Blue-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           52..94
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             100..102
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             112..156
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             172..186
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     promoter        236..254
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(342..358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(500..955)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        982..1086
                     /label=AmpR promoter
     CDS             1087..1944
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2118..2706
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2994..3015
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3030..3060
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3068..3084
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctc gcttgttctt
       61 tttgcagaag ctcagaataa acgctcaact ttggccacca tgggtgttac caaagaaacc
      121 gctgctgcta aattcgaacg ccagcacatc gacagcggta ccggtggcag cgatgacgac
      181 gacaagagcc cgggcttctc ctcaaaactg cagacgcgtt acgtatcgga tccttattta
      241 ggtgacacta tagaattccg atatctggtc gacaagcttc tcgagcctag gctagctcta
      301 gaccacacgt gtgggggccc gagctcgcgg ccgcacaatt cactggccgt cgttttacaa
      361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      481 agcctgaatg gcgaatggga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
      541 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
      601 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
      661 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
      721 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
      781 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
      841 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
      901 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
      961 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1021 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1081 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1141 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1201 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1261 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1321 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1381 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1441 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1501 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1561 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1621 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1681 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1741 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1801 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1861 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1921 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2881 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2941 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     3001 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     3061 tgtggaattg tgagcggata acaatttcac acaggaaaca gct
//
