LOCUS       62056_20860        3484 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP7(02V0002G3), complete sequence.
ACCESSION   MN689784
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3484)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..298
     gene            299..1288
                     /gene="VP7"
                     /label=VP7
     CDS             299..1288
                     /codon_start=1
                     /transl_table=11
                     /gene="VP7"
                     /product="VP7"
                     /label=VP7
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12453.1"
                     /translation="MYSTECTILSIEIIFYFIIVLLIYDIIHRMASSYVFCISVFIIAV
                     TILPRCHAQNYGVNVPITGSLDITVQNQTAEPIGLTSTLCLYYPAEASTEIADTEWKQT
                     ISQLFLTKGWPTTSIYFNEYQDLQTFSNNPSINCDYNIILIKYDGNQGLDISEIAELLL
                     YEWLCNEMDISLYYYQQTSEANKWIAMGTDCTVKVCPLNTQTLGIGCKTTDVSTFEQLT
                     TAEKLAIIDVVDGVNHKIDYTVTTCNVKNCMRLNQRENVAIIQVGGPEIIDVSEDPMVV
                     PKMQRVTRINWKRWWQVFYTIVDYINTIVQTMSRRSRSLNTSAYYFRV"
     3'UTR           1289..1315
     terminator      1476..1523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1692..1708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1756..1787
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1788..1890
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1891..2748
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      2823..2850
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2862..3449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcgg ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gcatttaaat tagtaatttc cgtttctcac cgcggttaga ctaattaaat
      301 gtatagtact gaatgtacta tcctttctat tgagataata ttctacttta ttatagtact
      361 attgatttat gatattattc atagaatggc tagctcatat gttttctgca ttagtgtttt
      421 cattattgct gtcactattc taccacgttg tcatgctcaa aattatgggg ttaatgttcc
      481 aatcacagga tcgttagaca ttactgtaca gaaccaaaca gctgaaccaa ttggattaac
      541 gtcaactttg tgtttatatt atccagcgga agcgtcaaca gaaatagctg atactgaatg
      601 gaagcagaca ataagccagt tatttttaac caaaggatgg ccaacgactt ctatttattt
      661 taatgaatat caagatttac aaacgttttc gaataatcca agtataaatt gtgattataa
      721 tataatacta ataaaatatg acggaaacca aggattagac atttcagaaa ttgctgagct
      781 acttctatat gaatggttgt gtaatgaaat ggatattagt ttgtattatt atcaacagac
      841 ttcagaggct aataaatgga tagcaatggg cacagattgt acagttaaag tatgcccact
      901 aaatactcag acactaggaa ttggctgcaa gactactgac gtttccactt ttgaacaact
      961 aacgactgca gaaaaattag ctattattga tgttgttgac ggcgttaatc ataagataga
     1021 ctatactgtg actacttgta acgttaagaa ttgtatgcga ttaaatcagc gtgaaaatgt
     1081 agcaataata caagttggtg gaccagagat tattgatgtt tcagaagatc caatggttgt
     1141 accaaaaatg caacgtgtaa ctagaattaa ttggaaaagg tggtggcaag tattttatac
     1201 cattgtcgat tacataaata ctatagtaca aactatgtct agaagatcgc gctcactgaa
     1261 tactagcgct tattacttta gagtatagac tcatattgaa aggattgatg tgaccgggtc
     1321 ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca
     1381 ctcggatggc taagggagag ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg
     1441 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1501 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg cggccgcaag
     1561 cttattaagg atcgacgaga gcagcgcgac tggatcagtt ctggacgagc gagctgtcgt
     1621 ccgacgcgtg atcttacggc attatacgta tgatcggtcc acgatcagct agattatcta
     1681 gtcagcttga tgtcatagct gtttcctgag gctcaatact gaccatttaa atcatacctg
     1741 acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta
     1801 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     1861 tcgagatttt caggagctaa ggaagctaaa atgagtattc aacatttccg tgtcgccctt
     1921 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1981 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2041 agcggtaaga tccttgagag tttacgcccc gaagaacgtt ttccaatgat gagcactttt
     2101 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2161 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2221 ctcacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2281 actgcggcca acttacttct ggcaacgatc ggaggaccga aggagctaac cgcttttttg
     2341 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2401 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2461 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2521 gcggataaag ttgcaggatc acttctgcgc tcggccctcc cggctggctg gtttattgct
     2581 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2641 ggtaagccct cccgcatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2701 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta atgagggccc
     2761 aaatgtaatc acctggctca ccttcgggtg ggcctttctt gaggacctaa atgtaatcac
     2821 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     2881 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     2941 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3001 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3061 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3301 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     3361 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3421 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg
     3481 ccca
//
