LOCUS       62056_20855        3758 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP6(02V0002G3), complete sequence.
ACCESSION   MN689783
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        225..243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           242..264
     gene            265..1458
                     /gene="VP6"
                     /label=VP6
     CDS             265..1458
                     /codon_start=1
                     /transl_table=11
                     /gene="VP6"
                     /product="VP6"
                     /label=VP6
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12452.1"
                     /translation="MDVLYSLAKTLKEARAKIVEGTLYTNVADIVQQINQVINSLNGST
                     FQTGGIGNFQIRDWAFDFGALGTTLLNLDANYVENARATIDYFIDFVDHVCIDEIVRES
                     QRNGIAPQSNALRQLSAARYRRINYDNDSEYIENWNLQNRRQRTGYLFHKPNILPYNNS
                     FTLTRSQPAHDNVCGTMWLNNGSEIEIIGFDSECALNAQANVQAFQHVVPLGRVLNNAT
                     VSLLPYAPRLMQRAVIPTADGQNTWLFDPVMLRPHNPQIEFLLNGQVITVYQARYGTLS
                     ARNFDTIRLSFQLVRPQNMTPAVAALFPVAAPFPNHAAVGLTLNIDSALCESVLTDANE
                     PYLSIVTGLRQEYAIPVGPVFPAGMNWTELLNNYSASREDNLQRIFTVASIRSMVIK"
     3'UTR           1459..1589
     terminator      1750..1797
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1966..1982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2030..2061
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        2062..2164
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2165..3022
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3097..3124
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3136..3723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atggaacctg caggtaatac gactcactat
      241 aggctttaaa acgaagtctc catcatggat gtactttatt cgctcgccaa aactctaaaa
      301 gaagctagag caaaaattgt tgaaggtact ctttacacta atgtggctga tattgtgcag
      361 caaataaatc aagttattaa ttctcttaat ggttcaactt ttcaaacggg aggcattggt
      421 aactttcaaa ttagagattg ggcttttgat tttggtgctc ttggtactac actgctaaat
      481 ttggacgcaa attatgttga aaatgcgaga gcaactattg attacttcat tgattttgtt
      541 gatcatgtat gtatcgatga aatagtccgt gaatctcaac gtaatggaat agctcctcaa
      601 tctaatgcac taaggcagct ttcagcggcc agatatagaa gaataaatta tgataatgac
      661 tcagagtata tagaaaattg gaatttgcag aacagaagac aacgtacagg ttatcttttt
      721 cataaaccaa atattctacc atataataat tcattcactt taactagatc tcagccagct
      781 catgataatg tttgtggtac gatgtggcta aataatggtt cggaaataga gattataggt
      841 tttgattcgg aatgtgcatt aaatgcgcaa gcgaacgttc aagcgtttca acacgtcgta
      901 ccattaggac gagtacttaa caatgcaact gtttcacttc ttccttatgc accacgactt
      961 atgcagagag cagttattcc aacagctgac ggtcagaata catggctatt cgatccagtt
     1021 atgctaagac cgcataatcc acaaattgaa tttctattaa atgggcaagt aataactgtc
     1081 taccaagcta gatatggtac actatcagcg cgcaattttg atactattag actctcattt
     1141 cagctcgtta gaccacagaa tatgacacca gccgtagcag cacttttccc agtggcagca
     1201 ccatttccta atcatgcagc agttggactt actcttaata ttgattcagc tctgtgtgaa
     1261 tcagtgctaa cagatgctaa tgaaccgtac ttgtcaattg ttaccggact aagacaagag
     1321 tatgcgattc ctgttggacc agtatttcct gctggtatga attggaccga attgcttaac
     1381 aattattcag cctcgaggga agataatctg caacgtatat ttacagtagc atccattcgg
     1441 agcatggtca ttaagtagag attgagggta acagctcata ccaaagctta atggtcatgt
     1501 agctatactg acgaatagtc cgtagcggac gtaagcattg cgcgctaccc gtgcaaggac
     1561 ttgcagcagt atagtgagag gatgtgaccg ggtcggcatg gcatctccac ctcctcgcgg
     1621 tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg agagctcaaa
     1681 aaaaaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1741 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1801 aaaggaggaa ctatatccgg atcgcggccg caagcttatt aaggatcgac gagagcagcg
     1861 cgactggatc agttctggac gagcgagctg tcgtccgacc cgtgatctta cggcattata
     1921 cgtatgatcg gtccacgatc agctagatta tctagtcagc ttgatgtcat agctgtttcc
     1981 tgaggctcaa tactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc
     2041 tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga
     2101 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     2161 taaaatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagtttacg
     2341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2461 cttggttgag tactcaccag tcacagaaaa gcatctcacg gatggcatga cagtaagaga
     2521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctggcaac
     2581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gatcacttct
     2821 gcgctcggcc ctcccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgca tcgtagttat
     2941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3001 tgcctcactg attaagcatt ggtaatgagg gcccaaatgt aatcacctgg ctcaccttcg
     3061 ggtgggcctt tcttgaggac ctaaatgtaa tcacctggct caccttcggg tgggcctttc
     3121 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     3181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3601 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3721 caagaagatc ctttgatttt ctaccgaaga aaggccca
//
