LOCUS       62056_20850        5001 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP3(02V0002G3), complete sequence.
ACCESSION   MN689782
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 5001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..298
     gene            299..2788
                     /gene="VP3"
                     /label=VP3
     CDS             299..2788
                     /codon_start=1
                     /transl_table=11
                     /gene="VP3"
                     /product="VP3"
                     /label=VP3
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12451.1"
                     /translation="MKVIALRRDLVSSYADTQIYKHDESKDYYENAYLISNLTSHNVLY
                     INYYIQVLEILNKSGVASIIATNSEELNLLIKSNYTYDYYRNILYLHDYSYYTLNELRT
                     DQFWLTTTNINDYLPPGWKLTYVGYLGIKTRGHYTHSFTCQNTATDDDIIYDYIYSDET
                     DFLPFLLQALNKRLTSALNYHRLSNRVLRELLYLSVPKNTINIGPRNESMFTLLKYPNI
                     TNYPANEMYVSDLIKLPQEKWVGAENTQFDIGQFKNMCNVLSTIYTYYNKYHDYPKIYM
                     VGSAPSYWIKDLLEIHKFTFETWDPLDTPYSTNHHFEMFSLTDVDKLTDNSILYIDIRT
                     DRNGMDWKQWRTKVEEETKINLDMMYRYLSKGQNRICCCKITAMDIELPISATLLHFPT
                     TKIRSECYILCTKESLLDKKRFVPKGSFYAFINNNKTDNVFFGSEYRLKPTNKFVVALY
                     SLSNENNDRDKMLSFLKKQKNGVITLRVNNTFNYAYRLQFKQTYDYLYLPTDIMRDDTI
                     VTSYDGYAAMHNLSCSLEGKATGNNHLFVSFSDKNYYNIDLYCNHMGISRRSHSIRFSE
                     AATTLSGYMFRDLTNGKFSLINTNVENVVSGHVYNALIYFRYNYRFDLLRWINLHAMNK
                     VSILGGKYYEHAPPELLYACQSALTFAKLQNDLTLIEYVKSVQDFIHDKYKITYADDPN
                     YYISIEFVDLPYKSEVKRPHLTGGLLLINDTEIPTVIEILQTVKEDILMLELTTQYTYM
                     LTDSHYVANVNGYLQFYYKLYMMFYRKNITFGQSRMYIPHITLAKVKKNAIRIDSSRII
                     VKSIFLSQIGTDVEYRI"
     3'UTR           2789..2832
     terminator      2993..3040
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3209..3225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3273..3304
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        3305..3407
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3408..4265
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4340..4367
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(4379..4966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gctattaaag ctacacgagt agtacgtctt actcttagtg gtgtagatat
      301 gaaagtgata gctttaagac gcgacttggt ctcatcttat gcggatactc agatctataa
      361 acacgacgaa tctaaagact attatgaaaa tgcatactta atctcaaatt taacttcaca
      421 taatgttctt tatattaatt actacattca agttttagag atattaaata aatcgggtgt
      481 tgcatcaata atagctacta attctgagga attgaattta cttattaagt ctaattatac
      541 ttacgattat tacaggaata tattatattt gcatgattat tcatattata cgttgaatga
      601 actaagaact gatcaatttt ggttaacaac tacgaatata aatgattatt taccaccagg
      661 ctggaaattg acctatgtag gctatttagg aataaaaact agaggtcatt acacccatag
      721 tttcacgtgt caaaacactg ctactgacga tgatattata tacgactata tttattctga
      781 cgaaacagat tttttaccat ttcttctaca agctttaaat aaaaggttaa catcagcatt
      841 aaattatcat agattgtcaa atagagtatt aagagaattg ttgtatttat cagttccaaa
      901 gaatactata aatattggtc caagaaatga atctatgttt acgttgttaa aatatcctaa
      961 cataactaat tatccagcaa atgagatgta tgtgtcagac ctaattaaac taccacaaga
     1021 gaaatgggtt ggagcagaaa atacgcaatt tgatattggt caatttaaaa atatgtgtaa
     1081 tgtattgtca actatttata cttattataa caaatatcat gattacccta aaatatatat
     1141 ggttggttca gcgccatctt attggattaa agatttactg gaaattcaca aatttacatt
     1201 cgaaacttgg gatcctttgg atactcccta ctcaacaaat catcattttg agatgttctc
     1261 attgactgat gtagataaat taactgacaa ttcaatactt tatatagaca ttagaacaga
     1321 tagaaatgga atggattgga agcagtggag gacgaaagtt gaagaagaaa ctaagattaa
     1381 cttggatatg atgtatagat atttatccaa gggacagaat agaatttgtt gttgtaaaat
     1441 tacagcaatg gacattgaat taccaattag tgctacattg cttcattttc cgacaactaa
     1501 gataagatcg gaatgctata ttttgtgcac taaagagtca ttgttagata aaaaaagatt
     1561 tgtaccaaag ggatcgtttt atgcatttat aaataataat aaaactgaca atgtgttttt
     1621 tggttcagag tatagattaa aaccgacaaa taaatttgta gtagcattat attcgctatc
     1681 taatgaaaat aatgacagag ataaaatgtt atcattctta aaaaagcaaa agaatggtgt
     1741 tataacatta cgcgtaaata atacatttaa ttatgcttat agattgcagt ttaaacaaac
     1801 atatgattat ttatatttgc caactgatat aatgagagac gatacgattg ttacttcgta
     1861 tgatggatac gctgctatgc ataatttatc atgttcattg gaaggtaagg ccacaggtaa
     1921 taatcatctt tttgtctcat tctcagataa gaattactat aacatagatt tatactgtaa
     1981 tcatatgggc atttcaagac gatcacattc aattagattt tcagaagcag caactacttt
     2041 atctggttat atgtttagag atctaaccaa tggaaaattc agtcttataa atacaaacgt
     2101 tgagaatgtt gtatcaggac acgtttataa tgcactaatt tattttaggt ataactatag
     2161 gtttgatctt ttaaggtgga taaatttgca tgcaatgaat aaagtatcta tattaggtgg
     2221 taaatattat gaacacgcac caccagaatt gctttatgcc tgtcagtcag cacttacatt
     2281 tgcaaagcta caaaatgatt taactctaat tgaatatgtc aaatcagttc aggattttat
     2341 acatgacaag tataaaataa cttacgcaga tgatcctaat tattatattt ctatagagtt
     2401 cgtagattta ccatataagt cagaagtaaa aagaccacat ttaactggtg gattattatt
     2461 aataaacgac actgaaattc caaccgtaat agaaatacta cagactgtga aggaagatat
     2521 actcatgtta gaactaacaa ctcaatatac atatatgctc acagattcac actatgtagc
     2581 aaatgtaaat ggttatctac aattttacta taaattgtat atgatgtttt ataggaaaaa
     2641 tataacattt ggacaatcaa gaatgtatat tccacatata acgttagcga aagtaaaaaa
     2701 gaatgcaatc cgtattgact cttcacgtat aatagttaag tcaatattct tgagtcagat
     2761 aggtacagat gttgagtatc ggatttaggt gagataggat aaaaacctaa caagtcagtc
     2821 acagatgtga ccgggtcggc atggcatctc cacctcctcg cggtccgacc tgggcatccg
     2881 aaggaggacg tcgtccactc ggatggctaa gggagagctc aaaaaaaagg atccggctgc
     2941 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     3001 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     3061 cggatcgcgg ccgcaagctt attaaggatc gacgagagca gcgcgactgg atcagttctg
     3121 gacgagcgag ctgtcgtccg acccgtgatc ttacggcatt atacgtatga tcggtccacg
     3181 atcagctaga ttatctagtc agcttgatgt catagctgtt tcctgaggct caatactgac
     3241 catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt
     3301 gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     3361 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg agtattcaac
     3421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt acgccccgaa gaacgttttc
     3601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3721 cagtcacaga aaagcatctc acggatggca tgacagtaag agaattatgc agtgctgcca
     3781 taaccatgag tgataacact gcggccaact tacttctggc aacgatcgga ggaccgaagg
     3841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4021 taatagactg gatggaggcg gataaagttg caggatcact tctgcgctcg gccctcccgg
     4081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4141 cagcactggg gccagatggt aagccctccc gcatcgtagt tatctacacg acggggagtc
     4201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4261 attggtaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttcttgag
     4321 gacctaaatg taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt
     4381 tccataggct ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc
     4441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4921 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4981 tttctaccga agaaaggccc a
//
