LOCUS       62056_20845        5150 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP2(02V0002G3), complete sequence.
ACCESSION   MN689781
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 5150)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..266
     CDS             1885..1896
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     3'UTR           2955..2981
     terminator      3142..3189
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3358..3374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3422..3453
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        3454..3556
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3557..4414
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4489..4516
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(4528..5115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gctattaaag ctcaagatgg cttacagaaa taagcgtaat caaaataaaa
      301 aagaaaagga acaggaagta aaattgtcaa aagatcaaca ggagcaagag aaagaaacga
      361 agcaaaatga gaaggctgat ttaaaatcaa aagtgtttga taaaaaagag gatgtaatta
      421 ctgatgaccc acagaaacaa ctagatccaa aaactaagga aagtggtgac agtgtgaagt
      481 tggataaaca aattattgag tctaagaaag aagattcaaa acagctagtt gaagtactaa
      541 aaactaaaaa agagcatgaa aaagaagtac aatatgaaat tctgcagaaa actattccaa
      601 catttcaacc aaacgaatcc atactcaaaa aaatgattga tattaaacca gatccaatta
      661 aaaaatcaga aaaactattt agattattcg aaccaaaaca attgccgatt tatcgtgcga
      721 atggagagag agagttacgt aatagatggt attggaaatt aaagaaagat gacttaccag
      781 aaggcgacta tgatgttaga gaatattttt tgcacttgta ttctcaagta ttagaatata
      841 tgccagatta tatgttgctg aaagaaatgg ccgtcgaaaa taagaattca agagatgctg
      901 ggaaagttgt ggataaagag acagctcaga tatgtgataa catctttcaa gatgaagaaa
      961 ctgaaggtgt cgttaggaga ttcatagcag atatgaggca acgagttaat gctgaacgca
     1021 atactgtaga ttatccagct attctacatc aaatagacta tgaatttaat caatactttt
     1081 tgacgcacca aattgtagaa ccgctaacta atgaggttat atataattac attcctgaga
     1141 gattacgaaa tgatccaaac tatattttga atatggatgt taatttgcca cctactgcca
     1201 ggtatataag accagtttta ctacaggata ggctgaattt acatgataat tttgaatcta
     1261 tatgggatac atttacaaaa gctaattata tattagcgag gtcagtagta ccagatttga
     1321 aagaattagt ttcaactgag gcgcaaattc agaagatgtc acaagattta caattggaag
     1381 cgctaactat tcaatcagag actcaatttc taactggcat aaattctcag gccgcaaatg
     1441 acgcgtttaa aacgataata gcttgcatgc tcgctcaaag aacaatgtca ataaaattcg
     1501 ttacatcaaa ttatatgtca ctcatatcca gtatgtggtt aatgtcagta gtacctacta
     1561 gtatgttcat tagagagtca ctcgttgctt gccagcttgc agtagtaaat acgatcatgt
     1621 atccagcttt tggactacaa aaaatgcatt acattaatgg tgatccaaga accccattta
     1681 tgattgctga acagcaaatt aataattttc aagtccagaa ctggttgcat tttgttaatc
     1741 ataacgcgtt taatcaagta gctattgatg gagtgattaa tcaaactctt aatgaccaaa
     1801 tacgcagagg aatggtaata aatcagttga tggaagcact aaatgtcatg gcaagacaaa
     1861 attttcaagc ttatccaata gattatagaa ggtcggtgca aagaggtata ctattattgg
     1921 ctaatagagt tggtcaatta gttgatttaa cgcgtttaat gtgctataat tatgaaactt
     1981 taatggcttg tttgactatg aatatgcaaa acgtgcagac tttaactaca gaggaattac
     2041 agctgacttc agttacttca ttatgtatgt tgattggaaa tactactgta attccggagc
     2101 caagaacact atttcactat tatcagacta acgttaattt tcacagtaac tataatgaaa
     2161 gaataaatga cgcagtcgca attgttgctg cagctaatca tctaaatttg tatcaaaaga
     2221 aaatgaagtt aattgttgag gatttcctta agaaattgca catttttgat gtatcaaaag
     2281 taccagacga tcaaatgtat agactgagag acagattgag acgattgcca gtagaaaggc
     2341 gaagattaga tgtatttgca ataatattaa ataatatgca acaaattgaa agagcttcgg
     2401 ataaaatagc tcaaggtgta attattgcat accgtgaaat gcaactagac tatgatgaac
     2461 tatatggatt cgtgaatttg gctagaaatt tagatggtta tcaaaggatt aatttagaag
     2521 aattaatgag atctggtgat tataatcaga ttacgaatat tttactgaat aatcaaccag
     2581 tcgcactagt cggagctatt ccatttataa ctgattcagc agtcacttcg ttaatagcta
     2641 aattagacgc tacagtattt gctcagattg taaagcaaag gaaagttgat acaatcaaac
     2701 cgattctatt taaaataaat tctgattcta atgattttta tctagttgta aattataatt
     2761 gggtgccgac ttctacgacg aaggtattta aacaagttcc acaagttttt gactttagag
     2821 ccgctatgca tgtcctctcg tcgaacttaa ctttcacaat gtttcatgaa ttactaccat
     2881 ttgttaatgc cgacactgtt gagcccatta atgctgttgc atttgacaac attagaatta
     2941 tgcaagaact gtaagcctga accgtaaatg gagatatgac cgggtcggca tggcatctcc
     3001 acctcctcgc ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag
     3061 ggagagctca aaaaaaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     3121 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     3181 ggttttttgc tgaaaggagg aactatatcc ggatcgcggc cgcaagctta ttaaggatcg
     3241 acgagagcag cgcgactgga tcagttctgg acgagcgagc tgtcgtccga cccgtgatct
     3301 tacggcatta tacgtatgat cggtccacga tcagctagat tatctagtca gcttgatgtc
     3361 atagctgttt cctgaggctc aatactgacc atttaaatca tacctgacct ccatagcaga
     3421 aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt
     3481 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     3541 agctaaggaa gctaaaatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3601 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3661 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3721 tgagagttta cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3781 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3841 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctca cggatggcat
     3901 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3961 acttctggca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4021 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4081 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4141 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4201 aggatcactt ctgcgctcgg ccctcccggc tggctggttt attgctgata aatctggagc
     4261 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4321 catcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4381 cgctgagata ggtgcctcac tgattaagca ttggtaatga gggcccaaat gtaatcacct
     4441 ggctcacctt cgggtgggcc tttcttgagg acctaaatgt aatcacctgg ctcaccttcg
     4501 ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4561 caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4981 tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc
     5041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5101 aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
//
