LOCUS       62056_20840        5711 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP1(02V0002G3), complete sequence.
ACCESSION   MN689780
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        221..239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           238..255
     gene            256..3525
                     /gene="VP1"
                     /label=VP1
     CDS             256..3525
                     /codon_start=1
                     /transl_table=11
                     /gene="VP1"
                     /product="VP1"
                     /label=VP1
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12449.1"
                     /translation="MGTYNVLLSEYLQFLYTSNDFIQIPIYYSANNELEKRCIEFHEKA
                     VLLSKNNQSIKNLYTEYKDVIDNASLLSVLSYSYGKYGNVENKLKEYATVAPLEAKHIN
                     DLDYENNKLTRELFTESNYTDSLMDPAILTSLSSNLNAAMFWFHHNKGKLSSNVEFAKT
                     YRRREALFRIVASTVNKYGVPRHDQKYRYTYEVMKDKPYYLVTWANSSIEMLMSVHDHN
                     DFLIAEELIINSYSNRSTLAKLVSSPMSILVALLDINGTFITTEELELEFSNKYVKAIV
                     PQKTLDELEEMLVSMEKANLYNIPKLIREWISTPTLSGFKLMAKIYSFSFHVGFRKQKM
                     IDAALDQLRTEYSTNVDNEMYQEYSMLIRDEVVKMLQESVIHEDHLLIDSELAGLLSMS
                     SASNGESRQLKFGRKTIFSTKKNMHVMDDMYNGRYNPSIVPKVDQTSPIPLGRRDVPGR
                     RTRIIFILPYQYFLAQHAIVEKMLQYAKHTREYAEFYSQSNQLLSYGDVTRFLSDDALV
                     LYTDVSQWDSSQHNTEPFRKGIIAGLDKLMTMTNNEGIKSTLQKYKQTQINLMDSYVQI
                     PDGEKILKIQYGAVASGEKQTKAANSIANLALIKTVLSRLANQHAFKTKVIRVDGDDNY
                     AVLQFNSKITPELVINVSDFIRDTYSRMNAKVKALVSTVGIEIAKRYIAGGKIFFRAGI
                     NLLNNEKRGQNTQWDQAAVLYANYIVNRLRGFYTDRDFILTKIMQMTSVAITGSLRLFP
                     SEKVLTTNSTFKVFDATDFIIEYGTSDDDVYLQRAFMSLSSQSSSIADEIASSQVFKNY
                     VSKVSNDLLKFNNSIVAKGIAQTEKAKLNSYAPIALEKRRAQLSSLLTMLQKPVRFKSA
                     KITINDILSDIKPYFKQDAADLVPIYPEFMPTLPANVQYVLRCIGSRTYQIEDDGSKST
                     ISKLIRKYSVYKPSVEELYKVISLQEKDIKLYLMSLGVPNIDSSAYIESKVYNQDKYRI
                     LESYIYNLLSINYGCYQLLDFNSKDLESLIRIPFKGKIPSVTFILHIYAKLHIVNYAIK
                     TGKWITLFCDFPKSEMVKLWKKMWSITSLRSPYSNANFFQE"
     3'UTR           3526..3542
     terminator      3703..3750
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3919..3935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3983..4014
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        4015..4117
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4118..4975
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      5050..5077
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(5089..5676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga taatacgact cactataggc
      241 tattaaagct atacgatggg gacgtataat gtcctactct cggagtattt acagttttta
      301 tatacatcaa atgattttat tcaaatacca atttattatt cagcaaataa tgaattagaa
      361 aaaagatgca ttgaatttca cgaaaaggcg gtattattgt caaagaacaa ccagtcaatt
      421 aaaaatctat atactgaata caaagatgtg atagataatg catctttatt atcagtattg
      481 tcttactcat atggaaaata tggtaacgtt gagaataagc ttaaagaata tgcaactgtt
      541 gcaccattgg aagctaaaca cataaatgat ttggattacg aaaataataa gctaacaagg
      601 gaactcttta ctgaaagcaa ttatactgat agtttaatgg atccagcaat actgacatca
      661 ttatcatcaa atttgaatgc agcaatgttt tggtttcacc ataacaaagg aaagcttagt
      721 tctaacgttg aatttgcaaa aacatataga cgaagggagg cactatttag gattgtcgca
      781 tcaactgtca ataaatacgg agttccaaga catgatcaaa aatatagata cacatatgaa
      841 gttatgaaag ataagccata ctatttagtt acgtgggcaa actcttcaat tgagatgcta
      901 atgtctgttc atgatcataa tgatttttta atagcagaag aattgattat aaactcatac
      961 tcaaatagat caacattagc aaaattagtt tcttcaccaa tgtcaatact agtagcgtta
     1021 cttgatatta atggaacgtt tataactact gaagaattgg aattggaatt ctctaataag
     1081 tatgttaaag ccattgtacc tcaaaaaaca cttgatgaat tagaggaaat gttagttagt
     1141 atggaaaaag ctaacttata taatatccct aaattaataa gagagtggat tagtactcca
     1201 actttaagtg gctttaagtt gatggcaaaa atatactctt tttcgtttca tgtgggtttt
     1261 agaaagcaaa aaatgataga tgcggcgcta gatcaattaa gaactgagta ttcaacgaat
     1321 gtagataatg aaatgtacca ggagtattcc atgttaatca gagatgaagt agttaaaatg
     1381 ttacaggaat ctgtaattca tgaagatcat ttactgattg attcagaatt agctggacta
     1441 ctatctatgt catcagcatc taatggtgaa tcgagacaac ttaagtttgg aagaaaaact
     1501 attttttcaa cgaaaaagaa catgcatgta atggatgata tgtataatgg tagatataat
     1561 ccctctattg ttccgaaagt cgatcaaact agtccaatac cattgggtag acgagatgta
     1621 cctggacgac gcacacgtat tatatttatt ttaccatacc agtatttttt agctcaacat
     1681 gctatagttg agaaaatgtt gcagtacgcc aaacatacac gagaatacgc tgagttttat
     1741 tcgcaatcta atcaattact ttcatatggt gacgttactc ggtttttatc tgatgatgct
     1801 ttagtgttgt atacagatgt atcacaatgg gattcatcgc agcataatac agaaccattt
     1861 agaaaaggta ttatagctgg tttagataaa ctaatgacta tgacaaacaa tgaagggatt
     1921 aagagtacct tacaaaaata taaacaaaca caaatcaatt tgatggactc ttatgttcaa
     1981 attcctgatg gcgagaaaat actaaaaatt caatatggag ccgttgcttc cggcgaaaag
     2041 caaacgaaag ctgctaattc tatagcaaat ctagcattaa ttaaaacagt gttatctagg
     2101 ttggctaatc agcatgcttt caaaacaaaa gttattagag ttgacggtga tgataactac
     2161 gctgttttac aatttaattc gaaaataact ccagaactag taataaatgt atctgatttt
     2221 attcgggata catactcacg aatgaatgcg aaagttaaag ctttagtctc aactgttgga
     2281 attgaaatag ctaagagata tattgcagga ggcaaaatat ttttccgcgc tggtataaat
     2341 ttattaaata atgaaaaacg tggacagaac acacagtggg atcaggctgc agtattatat
     2401 gcaaattata tagttaatcg gttgagaggc ttttatacgg acagagattt tattttaacc
     2461 aagataatgc aaatgacttc agtagctata actggctcat tacgcctatt tccatctgaa
     2521 aaagtactaa cgacaaattc gacatttaaa gtatttgatg ccacagattt cattattgaa
     2581 tatggtacct cagatgatga tgtttatcta caacgtgctt ttatgtcttt atcatcccaa
     2641 tcatcatcta ttgctgacga aattgcttcc tctcaagtgt ttaaaaatta cgttagtaaa
     2701 gtatcaaatg atctgctcaa atttaataat tcaatagtag caaaaggaat tgcacaaact
     2761 gaaaaggcta aattaaatag ctatgcacca attgcacttg agaaaagacg tgcgcaacta
     2821 tcatcactgt tgactatgct acagaaacca gttagattta aatctgcaaa aataacaata
     2881 aatgatatac tatcagatat taaaccatac tttaaacagg atgctgctga tctagttcca
     2941 atatatccag aatttatgcc gactttacca gcaaacgtgc aatatgtctt acgatgtata
     3001 ggttcacgta catatcaaat tgaggacgac gggtcgaaat caactatatc taaattaata
     3061 agaaaatatt cagtatataa gccttcagtt gaagaattgt ataaagttat ctcattgcag
     3121 gaaaaagaca ttaagttata tttaatgtca ctgggagtac caaatattga ctcgtcagca
     3181 tatatagaat ctaaagtgta taatcaagat aaatatcgta tactagaatc atatatttat
     3241 aatttacttt ccattaatta tggatgttac caattattag acttcaattc taaggattta
     3301 gaatcgctca tacgaatacc gtttaaagga aagatacctt ctgtaacctt tattttacat
     3361 atatatgcaa agttgcatat tgtgaattac gctattaaaa ctggtaaatg gatcacgtta
     3421 ttctgcgatt ttccaaaatc agagatggtc aaactatgga agaaaatgtg gagtattact
     3481 tcattaagat cgccatattc aaatgcgaac ttcttccaag agtaggccgc tacgatgtga
     3541 ccgggtcggc atggcatctc cacctcctcg cggtccgacc tgggcatccg aaggaggacg
     3601 tcgtccactc ggatggctaa gggagagctc aaaaaaaagg atccggctgc taacaaagcc
     3661 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     3721 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatcgcgg
     3781 ccgcaagctt attaaggatc gacgagagca gcgcgactgg atcagttctg gacgagcgag
     3841 ctgtcgtccg acccgtgatc ttacggcatt atacgtatga tcggtccacg atcagctaga
     3901 ttatctagtc agcttgatgt catagctgtt tcctgaggct caatactgac catttaaatc
     3961 atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg
     4021 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     4081 tgagttatcg agattttcag gagctaagga agctaaaatg agtattcaac atttccgtgt
     4141 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4201 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4261 tctcaacagc ggtaagatcc ttgagagttt acgccccgaa gaacgttttc caatgatgag
     4321 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4381 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4441 aaagcatctc acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4501 tgataacact gcggccaact tacttctggc aacgatcgga ggaccgaagg agctaaccgc
     4561 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4621 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4681 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4741 gatggaggcg gataaagttg caggatcact tctgcgctcg gccctcccgg ctggctggtt
     4801 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4861 gccagatggt aagccctccc gcatcgtagt tatctacacg acggggagtc aggcaactat
     4921 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaatg
     4981 agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttcttgag gacctaaatg
     5041 taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt tccataggct
     5101 ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc gaaacccgac
     5161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct cggaaaaaga
     5581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat tttctaccga
     5701 agaaaggccc a
//
