LOCUS       V002846                 4576 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002846
VERSION     V002846
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG,
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus
            Reveals a Function for Non-structural Glycoproteins Cleavage by
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
   PUBMED   25933376
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA
            30333, USA
REFERENCE   3  (bases 1 to 4576)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4576)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2015"; volume: "11"; issue: "5"; pages: "E1004879"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Viral Special Pathogens Branch, Centers for Disease
            Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30333, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..46)
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     promoter        705..809
                     /label="AmpR promoter"
     CDS             810..1667
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      2687..2704
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     promoter        2688..2705
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2706..2760
                     /label="viral UTR"
                     /note="viral UTR"
     CDS             2761..4206
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Crimean-Congo hemorrhagic fever
                     virus (strain Nigeria/IbAr10200/1970). Accession#: P89522"
     3'UTR           4210..4377
                     /label="viral UTR"
                     /note="viral UTR"
     ncRNA           4378..4471
                     /product="Hepatitis D virus ribozyme"
                     /label="other"
                     /ncRNA_class="other"
     terminator      4530..4576
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 gctgaaagga ggaactatat ccggatcgag atcctctagg tacaagccta attgtgtagc
       61 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
      121 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
      181 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
      241 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      301 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      361 cgtggccggg ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc
      421 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
      481 cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      601 gacaagctgt gacaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2641 cgcgttggcc gattcattaa tgcaggggga tctcgatccc gcgaaattaa tacgactcac
     2701 tatagtctca aagaaacacg tgccgcttac gcccacagtg ttctcttgag tgttagcaga
     2761 atggaaaaca agatcgaggt gaataacaaa gatgagatga acaggtggtt tgaagagttc
     2821 aaaaaaggaa atggacttgt ggacaccttc acaaactcct attccttttg cgagagtgtt
     2881 cccaatttgg acaggtttgt gtttcagatg gccagtgcca ccgatgatgc acagaaggac
     2941 tccatctacg catctgctct ggtggaggca acaaagtttt gtgcacctat atatgagtgc
     3001 gcatgggtta gctccactgg cattgtaaaa aagggacttg aatggttcga gaaaaatgca
     3061 ggaaccatta agtcctggga tgaaagttat actgagctaa aggtcgacgt cccgaaaata
     3121 gagcagctta ccggttacca acaagctgcc ttgaagtgga gaaaagacat aggtttccgt
     3181 gtcaatgcca acacagcagc tctgagcaac aaagtcctcg cagaatacaa agtccctggt
     3241 gagattgtga tgtctgtcaa agagatgctg tcagacatga ttaggagaag gaacctgatt
     3301 ctaaacaggg gtggtgatga gaacccacgt ggcccagtga gccatgagca tgtagactgg
     3361 tgcagggagt ttgtcaaagg caaatacatc atggccttca acccaccatg gggggacatc
     3421 aacaagtcag gccgttcagg aatagcactt gttgcaacag gccttgctaa gcttgcagag
     3481 actgaaggaa agggaatatt tgatgaagcc aaaaagactg tggaggccct caacgggtat
     3541 ctggacaagc ataaggacga agttgataga gcaagcgccg acagcatgat aacaaacctt
     3601 cttaagcata ttgccaaggc acaggagctc tataaaaatt catctgcact tcgtgcacaa
     3661 agcgcacaga ttgacactgc tttcagctca tactattggc tttacaaggc tggcgtgact
     3721 cctgaaacct tcccgacggt gtcacagttc ctctttgagc tagggaaaca gccaagaggt
     3781 accaagaaaa tgaagaaggc tcttctgagc accccaatga agtgggggaa gaagctttat
     3841 gagctctttg ccgatgattc tttccagcag aacaggattt acatgcatcc tgccgtgctt
     3901 acagctggta gaatcagtga aatgggagtc tgctttggga caatccctgt ggccaatcct
     3961 gatgatgctg cccaaggatc tggacacact aagtctattc tcaacctccg taccaacact
     4021 gagaccaata atccgtgtgc caaaaccatc gtcaagctat ttgaagttca aaaaacaggg
     4081 ttcaacattc aggacatgga catagtggcc tctgagcact tgctacacca atcccttgtt
     4141 ggcaagcaat ccccattcca gaacgcctac aacgtcaagg gcaatgccac cagtgctaac
     4201 atcatttaaa atacaaactg ctctgtactc aacttccttc cttctgaacc gccatccata
     4261 attgcaatac ttaatcatgc ttttttactt gcttatgtaa ccttatttta ttaacctttc
     4321 tctattttct cttgttttaa acacttaaag ggctgtgcgg caacgatatc tttgagaggg
     4381 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc
     4441 cactcggatg gctaagggag agctcggatc cggctgctaa caaagcccga aaggaagctg
     4501 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     4561 tcttgagggg tttttt
//
