LOCUS       62056_20795        3116 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP5(02V0002G3), complete sequence.
ACCESSION   MN689789
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3116)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..270
     gene            271..897
                     /gene="NSP5"
                     /label=NSP5
     CDS             271..897
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP5"
                     /product="NSP5"
                     /label=NSP5
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12458.1"
                     /translation="MSAITIDLSSLPSFSQFSNNESSSDVSNNSSKSVSRSEQYVTLSD
                     KAFSEYMLNKDPEDIGPSDSASNDLPTKFAIKANAVKSNANVGVSLDTKSDVKEKIMED
                     KDEVDFSFARNFKMNTDLAASITIDTHKPKSSLSDSAVQFNRKAKQGRSKIKKTEWPKI
                     DYQSDDEEYVCSDSEDRCCNCIYKKKYFELRKRMKQVAIQLIEDM"
     3'UTR           898..948
     terminator      1108..1155
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1324..1340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1388..1419
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1420..1522
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1523..2380
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      2455..2482
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2494..3081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gctttaaaag cactaccgtg atgtctgcaa taacgattga cttatcatct
      301 cttccatcat tttctcagtt ttcgaataat gaatcatcaa gtgatgtatc aaataactca
      361 agtaaatctg ttagtagaag tgaacaatat gtaacattat cagataaagc atttagtgag
      421 tatatgttaa acaaagatcc agaagatata gggccatcgg attcagcgtc taatgatttg
      481 ccaactaaat tcgccattaa agctaatgca gttaagtcaa acgcaaacgt aggagtatct
      541 cttgacacta aatcagacgt aaaggaaaaa ataatggaag ataaggatga ggttgacttt
      601 tcatttgccc gaaattttaa aatgaatact gatttagcag cgtctataac gattgatacg
      661 cataagccga aatcttcatt atcagactca gccgtgcaat ttaatagaaa ggctaaacaa
      721 ggaagatcca aaattaaaaa aacagagtgg ccaaaaattg attatcagtc agacgatgaa
      781 gaatatgttt gcagcgattc agaggataga tgctgtaatt gtatctataa gaagaagtat
      841 tttgaattac ggaagcgcat gaaacaagtt gcaatccagt taattgaaga tatgtaagtc
      901 aatttattgt ggactcgcga gggagatccc cactcccgtt ttgtgaccgg gtcggcatgg
      961 catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgtcgt ccactcggat
     1021 ggctaaggga gagctcaaaa aaaggatccg gctgctaaca aagcccgaaa ggaagctgag
     1081 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1141 ttgaggggtt ttttgctgaa aggaggaact atatccggat cgcggccgca agcttattaa
     1201 ggatcgacga gagcagcgcg actggatcag ttctggacga gcgagctgtc gtccgacccg
     1261 tgatcttacg gcattatacg tatgatcggt ccacgatcag ctagattatc tagtcagctt
     1321 gatgtcatag ctgtttcctg aggctcaata ctgaccattt aaatcatacc tgacctccat
     1381 agcagaaagt caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc
     1441 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     1501 ttcaggagct aaggaagcta aaatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1561 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1621 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1681 gatccttgag agtttacgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1741 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1801 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atctcacgga
     1861 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1921 caacttactt ctggcaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1981 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2041 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2101 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2161 agttgcagga tcacttctgc gctcggccct cccggctggc tggtttattg ctgataaatc
     2221 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2281 ctcccgcatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2341 acagatcgct gagataggtg cctcactgat taagcattgg taatgagggc ccaaatgtaa
     2401 tcacctggct caccttcggg tgggcctttc ttgaggacct aaatgtaatc acctggctca
     2461 ccttcgggtg ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga
     3001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3061 cgcagaaaaa aaggatctca agaagatcct ttgattttct accgaagaaa ggccca
//
