LOCUS       62056_20790        3142 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP4(02V0002G3), complete sequence.
ACCESSION   MN689788
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3142)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..289
     gene            290..796
                     /gene="NSP4"
                     /label=NSP4
     CDS             290..796
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP4"
                     /product="NSP4"
                     /label=NSP4
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12457.1"
                     /translation="MENVTTINETLVEEVYNMTLGYFEHNVIIMKYFPFLASILTIIFT
                     AWKMGRSTLKMTKTVAGSGYKVIKVVVVTVFNCILRIFGSKTEIVSEDKMDVMASKILK
                     QIDEQVKVIEQLTKRELEQVKLLADIYELLKYKSEGNVPSETNRKAYETWSKDPYQPTR
                     AVSLN"
     3'UTR           797..973
     terminator      1134..1181
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1350..1366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1414..1445
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1446..1548
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1549..2406
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      2481..2508
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2520..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gctttaaaaa ctcaatatcg agagagcccg tgcggaaaga tggagaacgt
      301 caccaccatc aatgaaacac ttgttgagga ggtatataat atgacattag gctactttga
      361 gcataatgtt attataatga aatattttcc atttctagca tctattttga caattatttt
      421 tacagcatgg aagatgggta gatcgacgct gaaaatgaca aagacagttg ctggatctgg
      481 atataaagtg attaaagtag tagttgtgac agttttcaat tgcattctga ggatatttgg
      541 ctcaaaaact gaaattgtct cagaggataa aatggacgta atggcatcaa aaatacttaa
      601 acaaattgat gaacaggtta aagtgattga acagcttaca aaaagagaat tggaacaagt
      661 aaaattgctt gcagatattt atgaactact aaagtacaaa agtgaaggaa atgtaccctc
      721 tgagacaaat agaaaagcgt atgaaacatg gagtaaagat ccctaccaac caactcgtgc
      781 agtgtcattg aattgatggt tcatgtctac gttgtcgaaa gaggcgtaga atgacgggtg
      841 aacccatcgg acctgcatga acatggagaa gccaccatgt tcatgatcgg gtgtggcaag
      901 aagccttaat ccctggtacc ccaaccgagc caggctcggc ggatctggga agatatttgg
      961 gtttaatatg accgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc
     1021 gaaggaggac gtcgtccact cggatggcta agggagagct caaaaaaaag gatccggctg
     1081 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     1141 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     1201 ccggatcgcg gccgcaagct tattaaggat cgacgagagc agcgcgactg gatcagttct
     1261 ggacgagcga gctgtcgtcc gacccgtgat cttacggcat tatacgtatg atcggtccac
     1321 gatcagctag attatctagt cagcttgatg tcatagctgt ttcctgaggc tcaatactga
     1381 ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt
     1441 tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1501 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat gagtattcaa
     1561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1681 atcgaactgg atctcaacag cggtaagatc cttgagagtt tacgccccga agaacgtttt
     1741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1861 ccagtcacag aaaagcatct cacggatggc atgacagtaa gagaattatg cagtgctgcc
     1921 ataaccatga gtgataacac tgcggccaac ttacttctgg caacgatcgg aggaccgaag
     1981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2161 ttaatagact ggatggaggc ggataaagtt gcaggatcac ttctgcgctc ggccctcccg
     2221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2281 gcagcactgg ggccagatgg taagccctcc cgcatcgtag ttatctacac gacggggagt
     2341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2401 cattggtaat gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttcttga
     2461 ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt
     2521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg
     2581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 ttttctaccg aagaaaggcc ca
//
