LOCUS       62056_20785        3507 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP3(02V0002G3), complete sequence.
ACCESSION   MN689787
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..326
     gene            327..1241
                     /gene="NSP3"
                     /label=NSP3
     CDS             327..1241
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP3"
                     /product="NSP3"
                     /label=NSP3
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12456.1"
                     /translation="MEATTLSVKSILVAAFDCAFVTTKSFLDDRKIEYDENDIKIVLKQ
                     KMDFILEDSGVKANILGKAATIDQALSGKTASANRNANWMTTQYTVSKLDEDVNRLRML
                     LSSQNITQETRVLNSCFKVRRDPNKSSSYITCTKLLKEKLKRGEVIEVADDVEEKVIVT
                     DTIDWKAKFEKLKTRFQEKYDSWVVKARKVTSTIRSMQMVIANQQQIINSFEEKQIQKE
                     LEYKNRIEALISSIKCKIESKQFNTETKNDYIQELDMTTVIDTADGMESIEEFIHKLVD
                     DYSDSTVLLQELALRLGLLVSFE"
     3'UTR           1242..1338
     terminator      1499..1546
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1715..1731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1779..1810
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1811..1913
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1914..2771
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      2846..2873
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2885..3472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gcttttaaag tcacatcgag gcactgagac cattgctgtt cagcgctttg
      301 gtgatatcac tcatcttgtg ttcactatgg aggcaactac tctttcagta aagtcaattc
      361 ttgttgctgc atttgattgc gcatttgtga ctacaaagtc attccttgat gacagaaaga
      421 tagaatatga tgagaatgat attaaaattg tactcaagca aaaaatggat tttattcttg
      481 aagactctgg agtgaaggca aacattcttg ggaaagcggc gactatagat caggctctaa
      541 gtggaaagac agcatctgca aatagaaatg cgaattggat gactactcaa tatactgtct
      601 caaaattaga tgaagacgtg aataggttgc ggatgcttct atcatctcaa aatataacac
      661 aagagactag agttttgaat tcctgcttta aagtacgacg cgatccaaat aaatcatcaa
      721 gttacattac gtgcactaag ctactgaagg agaagttgaa acgtggagaa gtaattgaag
      781 tcgctgatga tgtggaagaa aaagtaattg ttactgatac aattgattgg aaagctaaat
      841 ttgaaaagct taagactaga tttcaagaaa aatacgatag ttgggtagtt aaagcacgta
      901 aagtgactag tacaataaga tcaatgcaaa tggttatcgc aaatcaacaa caaataataa
      961 attcattcga agaaaaacaa atacagaagg aacttgagta caagaatagg attgaagcac
     1021 taatttcatc catcaaatgt aaaattgagt caaaacagtt caataccgaa acgaaaaatg
     1081 attatataca agaactcgat atgactacag ttattgatac tgctgatgga atggaaagca
     1141 ttgaagagtt tattcataaa ctggttgacg attattctga ctctaccgta ctattacagg
     1201 aattggcctt acgacttgga ctattagtgt catttgaata ataatatatc accaaatctt
     1261 atcagcataa agtcccagtg tagttagaag ctaacttgat gttaaaaccg tgaataacgc
     1321 acgggacttt atgtgaccgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg
     1381 catccgaagg aggacgtcgt ccactcggat ggctaaggga gagctcaaaa aaaaggatcc
     1441 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     1501 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     1561 tatatccgga tcgcggccgc aagcttatta aggatcgacg agagcagcgc gactggatca
     1621 gttctggacg agcgagctgt cgtccgaccc gtgatcttac ggcattatac gtatgatcgg
     1681 tccacgatca gctagattat ctagtcagct tgatgtcata gctgtttcct gaggctcaat
     1741 actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag
     1801 gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1861 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagta
     1921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2041 gttacatcga actggatctc aacagcggta agatccttga gagtttacgc cccgaagaac
     2101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2221 actcaccagt cacagaaaag catctcacgg atggcatgac agtaagagaa ttatgcagtg
     2281 ctgccataac catgagtgat aacactgcgg ccaacttact tctggcaacg atcggaggac
     2341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2521 aacaattaat agactggatg gaggcggata aagttgcagg atcacttctg cgctcggccc
     2581 tcccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2641 tcattgcagc actggggcca gatggtaagc cctcccgcat cgtagttatc tacacgacgg
     2701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2761 ttaagcattg gtaatgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt
     2821 cttgaggacc taaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     2881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     2941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3361 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3481 tttgattttc taccgaagaa aggccca
//
