LOCUS       62056_20780        3460 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP2(02V0002G3), complete sequence.
ACCESSION   MN689786
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 3460)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..294
     gene            295..1242
                     /gene="NSP2"
                     /label=NSP2
     CDS             295..1242
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP2"
                     /product="NSP2"
                     /label=NSP2
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12455.1"
                     /translation="MAELACFCYPCDKDGMSVSRYSRSAIKCMLNAKIDKANSSLPYDT
                     LVYGLAPPPIYKKRFNDGNNSRGMNFDTEMYDKVADLITKILNGVKIGKDKASEIMNVP
                     ISVRHMENLIRRIENVDDIISDDPNLITKSVLIAMGLIKDQELTITAEGGDIVFQNAGF
                     TMWKLDYKSHILMPISDPNFIEYKITLNHTEPIDDKIVKELVAELRWQYNKFAVITHGK
                     GHYRVVRYSTVANHADRVFSTYRAIQKRNPSHKFNELDSRVIWINWAAFTKSMLNGMKL
                     EDSKRLLFTKMKPNDTSFKGVTSERKIDEVSLLG"
     3'UTR           1243..1291
     terminator      1452..1499
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1668..1684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1732..1763
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1764..1866
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1867..2724
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      2799..2826
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2838..3425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gcttttaaag cgtctcagtc gccgtttgag cttgcggtgt cgttatggct
      301 gagctagcct gcttttgcta tccctgtgac aaagatggaa tgtctgtttc tagatattct
      361 agaagtgcca ttaaatgtat gttaaacgca aagattgata aagctaattc atccttgcca
      421 tatgatacac tagtctatgg attggcacca ccaccaatct ataagaaaag atttaacgat
      481 ggaaataatt caagagggat gaattttgat actgaaatgt atgataaagt agctgatttg
      541 ataactaaaa tacttaatgg agttaaaatt ggtaaagata aggcgtcaga gattatgaat
      601 gtcccaattt cagttagaca tatggaaaat ttaattagac gtattgaaaa cgttgatgat
      661 ataatttctg acgatccaaa tttgataacg aagtcagtgt taatagcaat gggattaatt
      721 aaagatcagg agttaactat tactgccgaa ggtggggata tagtctttca gaacgcagga
      781 tttactatgt ggaaacttga ctataaatca cacattttaa tgccaatttc tgatcccaac
      841 tttatagaat acaaaattac tcttaatcat actgaaccga ttgatgataa aattgtaaag
      901 gaactagtag ctgaattaag atggcagtac aacaagttcg cagttattac tcatggcaaa
      961 ggacactata gagtagtacg atattcaact gtcgcaaatc atgctgatag agtgttttct
     1021 acttatagag ctattcagaa acgtaaccct tcacataaat tcaatgaatt agacagtaga
     1081 gtcatttgga ttaattgggc tgctttcact aaatcaatgc taaatggaat gaaattggag
     1141 gactcaaaga ggctactttt cactaaaatg aaaccaaatg atacatcttt taagggagtg
     1201 acgtcggaaa gaaaaataga tgaagtatca cttctgggct aaatttgttg tggggatgta
     1261 ttagcaggaa ttgaaagccc tttatgtgac cgggtcggca tggcatctcc acctcctcgc
     1321 ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag ggagagctca
     1381 aaaaaaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1441 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     1501 tgaaaggagg aactatatcc ggatcgcggc cgcaagctta ttaaggatcg acgagagcag
     1561 cgcgactgga tcagttctgg acgagcgagc tgtcgtccga cccgtgatct tacggcatta
     1621 tacgtatgat cggtccacga tcagctagat tatctagtca gcttgatgtc atagctgttt
     1681 cctgaggctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
     1741 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
     1801 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     1861 gctaaaatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagttta
     2041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2101 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2161 gacttggttg agtactcacc agtcacagaa aagcatctca cggatggcat gacagtaaga
     2221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctggca
     2281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2401 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggatcactt
     2521 ctgcgctcgg ccctcccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg catcgtagtt
     2641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2701 ggtgcctcac tgattaagca ttggtaatga gggcccaaat gtaatcacct ggctcacctt
     2761 cgggtgggcc tttcttgagg acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt
     2821 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2881 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3241 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3301 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3421 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
//
